Quantification of total serum transferrin and transferrin sialoforms in human serum; an alternative method for the determination of carbohydrate-deficient transferrin in clinical samples

Abstract

Carbohydrate deficient transferrin (CDT) is a biochemical marker for congenital disorders of glycosylation (CDG), chronic alcohol consumption, and forensic medicine diagnosis.

N-Glycosylation profiling of intact target proteins by high-resolution mass spectrometry (MS) and glycan analysis using ion mobility-MS/MS

“…An example of quantification of TFN at the intact protein level was performed by LC-ICP-MS, focusing on the different sialoforms present on the protein. 50 Qualitative analysis of TFN glycoforms by high-resolution MS has been performed by Van Scherpenzeel et al and applied to the diagnosis of congenital disorder of glycosylation (CDG). 51 In their work, a similar method employing bead-based immunoaffinity purification of TFN and nano-LC Q-ToF analysis was employed, resulting in the characterization of several glycoforms in CDG samples.…”

Immunoaffinity Capillary Electrophoresis in the Era of Proteoforms, Liquid Biopsy and Preventive Medicine: A Potential Impact in the Diagnosis and Monitoring of Disease Progression

“…The resulting electropherogram shows P2, P3 (trisialo-Tf), P4 (tetrasialo-Tf) and P5 (pentasialo-Tf). Variation in serum sialoform content can be correlated to different pathological states, such as carbohydrate-deficient transferrin (CDT) [249], congenital disorders of glycosylation (CDG) [250], and cancer [251]. These improvements having better data acquisition, in combination with IACE, might result in superior data reproducibility to monitor minor protein changes during the course of progression of a disease.…”

Update on the status of metrology for metalloproteins

“…A species specic spike material was produced and thoroughly characterised regarding its protein structure, Fe load and isotopic composition. 16,17 A similar approach was developed by Ordonez et al, who besides total TRF also quantied the sialoforms using postcolumn ID-ICP-MS. 18 Both methods shall be used for the certication of the SRM-909c reference material in future. To reduce the inuence of Fe background and low isotopic enrichment in the spike material, triple isotope dilution mass spectrometry (IDMS) was used for the quantication of total TRF.…”