Quantification of Total Phenolic Content, HPLC Analysis of Flavonoids and Assessment of Antioxidant and Anti-haemolytic Activities of Hibiscus rosa-sinensis L. Flowers in vitro

Purushothaman, Ayyakkannu; Meenatchi, Packirisamy; Saravanan, S.; Sundaram, Ramalingam; Saravanan, N.

doi:10.21276/ijprhs.2016.05.02

Cited by 12 publications

(10 citation statements)

References 34 publications

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“…[27]By comparing the results obtained here with those obtained by maceration, a conventional extraction technique, the proposed methodology showed similar recovery of TPC and TFC from HRS petals. [28][29][30] However, these values were significantly lower than those reported by Mak et al [31]for aqueous and ethanolic extracts of H. rosasinensis petals. It's important to highlight that TPC and TFC values obtained in the present study were close to some varieties of Hibiscus sabdariffa.…”

Section: Fig2: Total Anthocyanins Content (Tac In Mg Cy-3-glu/100 G) Obtained By Mae From Hrs Petalsmentioning

confidence: 72%

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Phytochemical analysis, antioxidant activity and in vitro ocular irritation of Hibiscus rosa-sinensis L. extracts

Barros¹,

Santos²,

Polidoro³

et al. 2020

IJAERS

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Hibiscus ssp. is a source of phytochemicals, mainly anthocyanins, widely known for its medicinal properties. The aim of this study was to develop a microwave-assisted extraction (MAE) method to extract bioactive compounds from yellow and red petals of Hibiscus rosa-sinensis L. (HRS) and evaluate their antioxidant and eye irritation potential. For MAE, the effect of microwave power, irradiation time, solvent concentration and solvent volume was tested. The antioxidant activity and eye irritation potential were evaluated. The extracts were analyzed by LC-DAD-ESI-IT-MS/MS. The acidified aqueous extraction was the best choice for the recovery of anthocyanins, while hydroethanolic was best extractor solvent to recovery flavonol and flavanols. MAE extracts showed efficient recovery: total phenolic content (28.4 and 23.2 mg GAE/g), total flavonoids content (44.8 and 34.5 mg RE/g),total anthocyanins content (4.1 and 16.3 mg Cy-3-glu/100 g) and extraction yields (29.5 and 23.2 %) and remarkable antioxidant activity (IC50=12.35 and 15.76 mg L -1 ) for yellow and red petal extracts, respectively. Main compounds were cyanidin-3-sophoroside and cyanidin-3,5-diglucoside, besides catechin, epicatechin and rutin. The extracts were classified as non-irritating ingredients. The data revealed HRS as a source of bioactive compounds and significant variations in phytochemical content among yellow and red petals of HRS.

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Section: Fig2: Total Anthocyanins Content (Tac In Mg Cy-3-glu/100 G) Obtained By Mae From Hrs Petalsmentioning

confidence: 72%

“…Rutin was identified in HRS extracts in former studies. [30,42,43] Furthermore, Pillai and Min [43] identified chlorogenic acid, caffeic acid, p-coumaric acid, ferulic acid and, quercetin in the HRS extracts. These polyphenolic compounds were not identified in the present study.…”

Section: Total Carotenoid Contents (Tcc)mentioning

confidence: 96%

Phytochemical analysis, antioxidant activity and in vitro ocular irritation of Hibiscus rosa-sinensis L. extracts

Barros¹,

Santos²,

Polidoro³

et al. 2020

IJAERS

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show abstract

“…Purushothaman et al. ( 2016 ) reported that the methanol extract of hibiscus flower contained quercetin (7.6 μg/g), kaempferol (361.9 μg/g), and myricetin (50.7 μg/g). Tohma et al.…”

Section: Resultsmentioning

confidence: 99%

Effects of polyphenol‐rich traditional herbal teas on obesity and oxidative stress in rats fed a high‐fat–sugar diet

Iftikhar

Hussain

Chatha

et al. 2022

Food Science & Nutrition

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“…Moreover, flower extract contains tannins (7.5±0.20 %), phenols (0.678±0.14%) and alkaloids (0.51±0.16 %) while the total phenol content in flowers was 735±46 mg gallic acid equivalent /100g. The four main types of flavanoids in petals are rutin, quercetin, kaempferol and myricetin (Purushothaman, Meenatchi, S, Sundaram, & Saravanan, 2018). Petals contain various flavones such as quercetin-3-di-0-beta-D-glucoside, quercetin-3-7-di-0-beta-D-glucoside, cholesterol, campesterol, quercetin-3-0-beta-D-sophorotrioside, ß-sitosterol10, kaempferol-3-0-beta-D-xylosyl-glucoside19, catalase13.…”

Section: Phytochemicals In Flower On Blood Glucose and Serum Cholesterolmentioning

confidence: 99%

Hibiscus rosa-sinensis L. (red Hibiscus) Tea, Can It Be Used as A Home-Remedy to Control Diabetes and Hypercholesterolemia?

et al. 2021

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According to traditional medicine, Hibiscus is used to treat hypertension, heart diseases and many other ailments. Commercially available Hibiscus tea is prepared with H. sabdariffa and is expensive. H. rosasinensis is the common variety which is abundant in tropical home-gardens, however the scientific data are lacking on the effects. The present review focuses on the available scientific data on the effects of H. rosasinensis on controlling diabetes and hypercholesterolemia. The endeavour was to identify whether H. rosasinensis flower petals as a ‘tea’ is effective for diabetic and hypercholesterolemic patients. Medical databases such as MEDLINE, BMC, BMJ, research gate, Mendelay literature search database, Google scholar and the general engine Google were searched from November 2018 to April 2019. Search was carried out using keywords such as “Hibiscus rosasinensis”, “antidiabetic effects”, “antihyperlipidemic effects”, “toxic effects”, “anti-inflammatory effects”, “phytochemicals in Hibiscus” etc. Data were critically analyzed to assess whether the effective doses of the research studies on a par with the doses present in H. rosasinensis teas. We found thirty-nine journal articles fulfilled the criteria. The data were categorized and extracted on uses of H. rosasinensis, anti-diabetic effects, anti-inflammatory effects, anti-hypercholesterolemic effects and its phytochemicals. The review revealed that the dose of H. rosasinensis petals present in a home-made Hibiscus tea is theoretically sufficient to elicit anti-hyperglycemic and anti-hyperlipidemic effects. Home-made Hibiscus tea is effective in controlling diabetes and hypercholesterolemia without causing acute toxicity.

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Quantification of Total Phenolic Content, HPLC Analysis of Flavonoids and Assessment of Antioxidant and Anti-haemolytic Activities of Hibiscus rosa-sinensis L. Flowers in vitro

Cited by 12 publications

References 34 publications

Phytochemical analysis, antioxidant activity and in vitro ocular irritation of Hibiscus rosa-sinensis L. extracts

Phytochemical analysis, antioxidant activity and in vitro ocular irritation of Hibiscus rosa-sinensis L. extracts

Effects of polyphenol‐rich traditional herbal teas on obesity and oxidative stress in rats fed a high‐fat–sugar diet

Hibiscus rosa-sinensis L. (red Hibiscus) Tea, Can It Be Used as A Home-Remedy to Control Diabetes and Hypercholesterolemia?

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