Quantification of Tocopherols and Tocotrienols in Portuguese Olive Oils Using HPLC with Three Different Detection Systems

Cunha, Sara C.; Amaral, Joana S.; Fernandes, José O.; Oliveira, M. Beatriz P.P.

doi:10.1021/jf053102n

Cited by 125 publications

(85 citation statements)

References 32 publications

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“…ods for the separation, determination and detection of vitamin E compounds (tocopherols and tocotrienols) have been used previously [18,20] . Among three detection methods such as ultraviolet, evaporative light scattering and fluorescence, the best results were obtained with fluorescence detectors [19,21] . Using HPLC methodology coupled with fluorescence detection, we were able to detect ␦ -tocotrienol and quantified it in small amounts (ng) in a linear range.…”

Section: Discussionmentioning

confidence: 99%

Vitamin E δ-Tocotrienol Levels in Tumor and Pancreatic Tissue of Mice after Oral Administration

et al. 2009

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Tocotrienols are natural vitamin E compounds that are known to have a neuroprotective effect at nanomolar concentration and anti-carcinogenic effect at micromolar concentration. In this report, we investigated the pharmacokinetics, tumor and pancreatic tissue levels, and toxicity of δ-tocotrienol in mice because of its anti-tumor activity against pancreatic cancer. Following a single oral administration of δ-tocotrienol at 100 mg/kg, the peak plasma concentration (Cmax) was 57 ± 5 μmol/l, the time required to reach peak plasma concentration (Tmax) was 2 h and plasma half-life (t1/2) was 3.5 h. The δ-tocotrienol was cleared from plasma and liver within 24 h, but delayed from the pancreas. When mice were fed δ-tocotrienol for 6 weeks, the concentration in tumor tissue was 41 ± 3.5 nmol/g. This concentration was observed with the oral dose (100 mg/kg) of δ-tocotrienol which inhibited tumor growth by 80% in our previous studies. Interestingly, δ-tocotrienol was 10-fold more concentrated in the pancreas than in the tumor. We observed no toxicity due to δ-tocotrienol as mice gained normal weight with no histopathological changes in tissues. Our data suggest that bioactive levels of δ-tocotrienol can be achieved in the pancreas following oral administration and supports its clinical investigation in pancreatic cancer.

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Section: Discussionmentioning

confidence: 99%

Vitamin E δ-Tocotrienol Levels in Tumor and Pancreatic Tissue of Mice after Oral Administration

et al. 2009

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“…Among many other foodstuffs, the colour of olive oils, a food staple in the Mediterranean basin and a major component of the praised Mediterranean diet, have been the subject of a myriad of studies in the last years (Ceballos, Moyano, Vicario, Alba, & Heredia, 2003;Escolar et al, 1994;Escolar, Haro, & Ayuso, 2007;Moyano et al, 2001;Moyano, Melgosa, Alba, Hita, & Heredia, 1999), which is connected with the renewed interest in such products due to the wholesomeness and the likely beneficial effects stemming from their chemical composition (Cunha, Amaral, Fernandes, & Oliveira, 2006;Lee, Thurnham, & Chopra, 2000;Ranalli, Cabras, Iannucci, & Contento, 2001;Tura et al, 2007). Food Research International 41 (2008) [513][514][515][516][517][518][519][520][521] Contents lists available at ScienceDirect In this study, we have assessed objectively the colour and the total pigment contents of 1700 samples of Spanish olive oils (with different composition and obtained from olives from different geographical areas and at three different stages of ripeness) with diverse objectives, ranging from the thorough characterization of their colour in two different globally-accepted uniform colour systems (CIELUV and CIELAB) to the establishment of statistical relationships between their colour parameters and pigment contents.…”

Section: Introductionmentioning

confidence: 99%

A comprehensive study on the colour of virgin olive oils and its relationship with their chlorophylls and carotenoids indexes (II): CIELUV and CIELAB uniform colour spaces

Moyano

Meléndez-Martínez

Alba

et al. 2008

Food Research International

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a b s t r a c tThe colour of up to 1700 virgin olive oil samples obtained from diverse Spanish varieties and from drupes at different maturity stages was analyzed by considering the CIELAB and CIELUV uniform colour spaces. When all the samples were considered altogether it was noticed that the range h uv was considerably wider numerically than that of h ab , and so was the range of C Ã ab to that of C Ã uv . Regarding the simple regressions analyses of the relationships colour coordinates-pigments it the best correlations were found between the carotenoid index and C Ã ab and b*. When multiple linear regression analyses were applied considering the sets of scalar coordinates (L*u*v* and L*a*b*) and angular coordinates (L*C Ã uv h uv and L*C Ã ab h ab ) separately as independent variables values of R 2 nearing 0.9 were obtained. However, the strongest correlations of all (R 2 > 0.9) were obtained by means of general linear model multiple regression models considering non-redundant CIELAB sets of coordinates and s Ã uv .

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“…Numerous studies have determined a high content of tocopherols in olive oils through various methods of extraction [57][58][59]. Dietary olive oil supplementation in mouse models has provided promising anti-inflammatory results.…”

Section: Vitamin Ementioning

confidence: 99%

Psoriasis and Fat-soluble Vitamins: A Review

Usedom¹,

Neidig²,

Allen³

2017

J Clin Exp Dermatol Res

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Fat-soluble vitamins exhibit numerous routes of immune modulation and specifically alter a variety of pathways in psoriasis pathology. Psoriasis, a chronic immune-mediated disease, often requires a combination of topical, oral, and occasionally, subcutaneous or intravenous therapies making disease management complex. With the complexity of treatment, patient adherence is reduced. Vitamin supplementation as an adjunctive treatment would minimize potential adverse effects from systemic medication, increase patient adherence and decrease overall cost. While vitamin A and D are effective topically, oral supplementation is not currently a mainstay of therapy although data exists to support such supplementation. Data on vitamin E and K implicate the significant role each has in the alteration of the inflammatory cascade responsible for psoriasis. Research showing fat-soluble vitamin deficiency in psoriasis exists and presents greater evidence for oral vitamin treatment in addition to first-line therapies. This review presents the mechanism of action in psoriasis of each fat-soluble vitamin and the data on the efficacy of oral fat-soluble vitamin supplementation in psoriasis and systemic inflammation. We also present fat-soluble vitamin deficiency data shown in psoriasis patients. With the evidence of their targeted mechanism of action, efficacy data with oral supplementation, and evidence of deficiency in patients, oral fat-soluble vitamins should be considered as an adjunct to therapy in psoriasis patients.

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Quantification of Tocopherols and Tocotrienols in Portuguese Olive Oils Using HPLC with Three Different Detection Systems

Cited by 125 publications

References 32 publications

Vitamin E δ-Tocotrienol Levels in Tumor and Pancreatic Tissue of Mice after Oral Administration

Vitamin E δ-Tocotrienol Levels in Tumor and Pancreatic Tissue of Mice after Oral Administration

A comprehensive study on the colour of virgin olive oils and its relationship with their chlorophylls and carotenoids indexes (II): CIELUV and CIELAB uniform colour spaces

Psoriasis and Fat-soluble Vitamins: A Review

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