Quantification of tissue amyloid content in AA amyloidosis by inhibition ELISA

An Evaluation of Antigen Retrieval Procedures for Immunoelectron Microscopic Classification of Amyloid Deposits

“…Subsequent characterization of the fibril protein is mandatory to link the deposits with an underlying disease and thus facilitate appropriate medical treatment. The fibril protein can be characterized by biochemical procedures such as high-performance liquid chromatography, electrophoresis, immunoblotting, immunodiffusion, or by immunohistochemistry (Linke 1985;Linke et al 1986;Westermark et al 1986;Yakar et al 1995;Röcken et al 1996;Kaplan et al 1993Kaplan et al ,1997.…”

A quantitative method for detecting deposits of amyloid A protein in aspirated fat tissue of patients with arthritis

“…An additional advantage will be the characterisation of the amyloid as the amyloid A protein type. Amyloid A protein has already been quantified in small samples (10–60 mg) of mouse and human necropsy tissues of the spleen 10. However, the clinician who treats patients with arthritis needs a procedure that is easy to perform routinely during life, which can be repeated at intervals, and with results that are reproducible and can be obtained within days.…”