Quantification of the transient and long-term response of Saccharomyces cerevisiae to carbon dioxide stresses of various intensities

Richard, Lannig; Guillouet, Stéphane; Uribelarrea, Jean‐Louis

doi:10.1016/j.procbio.2014.07.020

Cited by 8 publications

(7 citation statements)

References 58 publications

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“…Elevated CO 2 (50%) did not, by itself, affect the biomass yield or viability of S. cerevisiae as compared to those under reference conditions (Table 1), and, accordingly, triggered only a weak transcriptional response ( Figure 5). This result appears to contradict results from two independent previous studies on the same strain, performed at CO 2 levels of 50% and 79%, under fully respiratory conditions (Aguilera et al, 2005;Eigenstetter & Takors, 2017;Richard et al, 2014). This apparent discrepancy may be related to the lower specific growth rates applied in the present study (0.025 hr −1 and below, while the cited earlier studies used 0.10 hr −1 ).…”

Section: Discussioncontrasting

confidence: 99%

“…Production of the free acid prevents the need for coproduction of large quantities of gypsum (Abbott, Zelle, Pronk, & Van Maris, ; Chen & Nielsen, ). In contrast to most carboxylic‐acid producing prokaryotes, the yeast Saccharomyces cerevisiae can withstand both high CO 2 (Aguilera, Petit, De Winde, & Pronk, ; Eigenstetter & Takors, ; Richard, Guillouet, & Uribelarrea, ) and low pH (Della‐Bianca, de Hulster, Pronk, van Maris, & Gombert, ; Verduyn, Postma, Scheffers, & van Dijken, ). However, although S. cerevisiae grows at high CO 2 , reduced biomass yields have been reported for respiring S. cerevisiae cultures grown at CO 2 values of 50% and 79% (Aguilera et al, ; Eigenstetter & Takors, ; Richard et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…In contrast to most carboxylic‐acid producing prokaryotes, the yeast Saccharomyces cerevisiae can withstand both high CO 2 (Aguilera, Petit, De Winde, & Pronk, ; Eigenstetter & Takors, ; Richard, Guillouet, & Uribelarrea, ) and low pH (Della‐Bianca, de Hulster, Pronk, van Maris, & Gombert, ; Verduyn, Postma, Scheffers, & van Dijken, ). However, although S. cerevisiae grows at high CO 2 , reduced biomass yields have been reported for respiring S. cerevisiae cultures grown at CO 2 values of 50% and 79% (Aguilera et al, ; Eigenstetter & Takors, ; Richard et al, ). Similarly, S. cerevisiae can grow at pH values as low as pH 2.5, but only at significantly reduced specific growth rates (Carmelo, Bogaerts, & Sá‐Correia, ; Della‐Bianca & Gombert, ; Della‐Bianca, de Hulster, Pronk, van Maris, & Gombert, ; Eraso & Gancedo, ; Orij, Postmus, Beek, Brul, & Smits, ).…”

Section: Introductionmentioning

confidence: 99%

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Physiological responses of Saccharomyces cerevisiae to industrially relevant conditions: Slow growth, low pH, and high CO₂ levels

Hakkaart

Liu

Hulst

et al. 2020

Biotech & Bioengineering

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Engineered strains of Saccharomyces cerevisiae are used for industrial production of succinic acid. Optimal process conditions for dicarboxylic-acid yield and recovery include slow growth, low pH, and high CO 2 . To quantify and understand how these process parameters affect yeast physiology, this study investigates individual and combined impacts of low pH (3.0) and high CO 2 (50%) on slow-growing chemostat and retentostat cultures of the reference strain S. cerevisiae CEN.PK113-7D. Combined exposure to low pH and high CO 2 led to increased maintenance-energy requirements and death rates in aerobic, glucose-limited cultures. Further experiments showed that these effects were predominantly caused by low pH. Growth under ammonium-limited, energy-excess conditions did not aggravate or ameliorate these adverse impacts. Despite the absence of a synergistic effect of low pH and high CO 2 on physiology, high CO 2 strongly affected genome-wide transcriptional responses to low pH. Interference of high CO 2 with low-pH signaling is consistent with low-pH and high-CO 2 signals being relayed via common (MAPK) signaling pathways, notably the cell wall integrity, high-osmolarity glycerol, and calcineurin pathways. This study highlights the need to further increase robustness of cell factories to low pH for carboxylic-acid production, even in organisms that are already applied at industrial scale.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Physiological responses of Saccharomyces cerevisiae to industrially relevant conditions: Slow growth, low pH, and high CO₂ levels

Hakkaart

Liu

Hulst

et al. 2020

Biotech & Bioengineering

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“…This phenomenon was in the focus of recent studies. Richard et al ( 2014 ) outlined that transient metabolic responses are triggered by CO 2 shifts e.g., characterized by intermediary increase of respiration rates and the excretion of ethanol and acetate.…”

Section: Co 2 Induced Growth Phenotypesmentioning

confidence: 99%

CO2 – Intrinsic Product, Essential Substrate, and Regulatory Trigger of Microbial and Mammalian Production Processes

Blombach

Takors

2015

Front. Bioeng. Biotechnol.

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Carbon dioxide formation mirrors the final carbon oxidation steps of aerobic metabolism in microbial and mammalian cells. As a consequence, CO2/HCO3− dissociation equilibria arise in fermenters by the growing culture. Anaplerotic reactions make use of the abundant CO2/HCO3− levels for refueling citric acid cycle demands and for enabling oxaloacetate-derived products. At the same time, CO2 is released manifold in metabolic reactions via decarboxylation activity. The levels of extracellular CO2/HCO3− depend on cellular activities and physical constraints such as hydrostatic pressures, aeration, and the efficiency of mixing in large-scale bioreactors. Besides, local CO2/HCO3− levels might also act as metabolic inhibitors or transcriptional effectors triggering regulatory events inside the cells. This review gives an overview about fundamental physicochemical properties of CO2/HCO3− in microbial and mammalian cultures effecting cellular physiology, production processes, metabolic activity, and transcriptional regulation.

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“…Although the yeast needed to use CO 2 to synthesize pyrimidine, purine, and a variety of amino acids, the CO 2 generated by respiration in the reactors was adequate to be supplied during the whole fermentation process. Reversely, excessive CO 2 played a detrimental role in yeast growth in many cases . Selecting strong base sodium hydroxide as the pH regulator would directly damage yeast walls as well as break the balance of internal and external electric potential difference of yeast.…”

Section: Discussionmentioning

confidence: 99%

High cell density culture of baker's yeast FX‐2 based on pH‐stat coupling with respiratory quotient

Huang

et al. 2019

Biotech and App Biochem

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The high cell density culture of baker's yeast FX-2 was investigated in a 50 L(A) automatic bioreactor. Herein, it was found firstly that the Crabtree effect clearly existed in batch fermentation with higher glucose content, then the critical initial glucose content range (ࣘ2.00 g L −1 ) was reasonably ascertained to effectively avoid Crabtree effect. In the next fed-batch fermentations with different strategies, the second strategy (maintain ethanol concentration lower than 0.10% and pH around 4.80) was confirmed to be more beneficial to yeast growth than the first strategy (keep reducing sugar not more than 2.00 g L −1 and control steady Carbon/Nitrogen ratio 3.05:1.00). After that, one optimal control strategy (maintain pH around 4.80 and keep respiratory quotient in the range of 0.90-1.00) was constructed to further enhance cell yield. Under an optimal control strategy, four schemes with the aim of achieving pH-stat were compared, and yeast extract instead of other alkaline materials was selected as a better regulator. As a result, 148.37 g L −1 dry cell weight, 38.25 × 10 8 mL −1 living cells, and 8.24 g L −1 h −1 productivity were harvested, which respectively elevated 23.74%, 135.38%, and 24.47% compared to that obtained under the traditional scheme (regulate pH with ammonia); meanwhile, the maximum oxygen uptake rate and carbon dioxide excretion rate were both more than 250.00 mmol L −1 min −1 .

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Quantification of the transient and long-term response of Saccharomyces cerevisiae to carbon dioxide stresses of various intensities

Cited by 8 publications

References 58 publications

Physiological responses of Saccharomyces cerevisiae to industrially relevant conditions: Slow growth, low pH, and high CO₂ levels

Physiological responses of Saccharomyces cerevisiae to industrially relevant conditions: Slow growth, low pH, and high CO₂ levels

CO2 – Intrinsic Product, Essential Substrate, and Regulatory Trigger of Microbial and Mammalian Production Processes

High cell density culture of baker's yeast FX‐2 based on pH‐stat coupling with respiratory quotient

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Quantification of the transient and long-term response of Saccharomyces cerevisiae to carbon dioxide stresses of various intensities

Cited by 8 publications

References 58 publications

Physiological responses of Saccharomyces cerevisiae to industrially relevant conditions: Slow growth, low pH, and high CO2 levels

Physiological responses of Saccharomyces cerevisiae to industrially relevant conditions: Slow growth, low pH, and high CO2 levels

CO2 – Intrinsic Product, Essential Substrate, and Regulatory Trigger of Microbial and Mammalian Production Processes

High cell density culture of baker's yeast FX‐2 based on pH‐stat coupling with respiratory quotient

Contact Info

Product

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About

Physiological responses of Saccharomyces cerevisiae to industrially relevant conditions: Slow growth, low pH, and high CO₂ levels

Physiological responses of Saccharomyces cerevisiae to industrially relevant conditions: Slow growth, low pH, and high CO₂ levels