Quantification of the Toxic Dinoflagellate Ostreopsis spp. by qPCR Assay in Marine Aerosol

Casabianca, Silvia; Casabianca, Anna; Riobó, Pilar; Franco, José M.; Vila, Magda; Penna, Antonella

doi:10.1021/es305018s

Cited by 62 publications

(55 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Another fundamental question concerns the process of aerosolization of the potential irritating agents (cells, fragments of cells or toxins, Casabianca et al 2013). Physical forcing associated with oceanic processes, such as swell (not studied here) and wind-generated turbulence should be major factors.…”

Section: Discussionmentioning

confidence: 99%

“…In the first study conducted on aerosol samples collected during the Ostreopsis cf. ovata blooms in Sant Andreu de Llavaneres (Catalan coast, NE Spain) in 2009 and 2010 (Casabianca et al 2013), PLTX-like compounds were not detected, although Ostreopsis cells were identified by molecular tools. In the sampling performed along the Tuscan coast (Italy) in the same years (Ciminiello et al 2014), a single positive result was obtained.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Establishing the link between Ostreopsis cf.ovata blooms and human health impacts using ecology and epidemiology

Vila¹,

Abós‐Herràndiz²,

Isern‐Fontanet³

et al. 2016

Sci. Mar.

Self Cite

View full text Add to dashboard Cite

Summary:Blooms of the benthic dinoflagellate Ostreopsis have been related to sporadic acute respiratory symptoms and general malaise in people exposed to marine aerosols on some Mediterranean beaches. However, the direct link between recurrent Ostreopsis blooms and health problems has not been clearly established. In order to establish and elucidate the connection, we conducted a joint ecology and epidemiology study in an Ostreopsis hot spot. Throughout the bloom, which extended from the end of June until the end of October 2013, 81% of the human cohort that we studied experienced at least one Ostreopsis-related symptom. Paradoxically, the time when the effects were greatest was during a short time window in early August. This corresponded to the transition from the exponential growth to the stationary phase of the bloom. Negligible symptoms were reported from August to mid-October, during the stationary period of the proliferation, when O. cf. ovata maintained high concentrations of epiphytic cells. No clear patterns in the landward wind component were noted during the time when health effects were greatest. Our main hypothesis is that the irritants present in the aerosol are produced during a particular physiological phase of the Ostreopsis cells during the bloom.Keywords: Ostreopsis, epidemiology, Mediterranean, HAB, dinoflagellates, respiratory irritation.Aproximación ecológica y epidemiológica para establecer la relación entre las proliferaciones de Ostreopsis cf. ovata y sus impactos sobre la salud humana Resumen: Las proliferaciones del dinoflagelado bentónico Ostreopsis en algunas playas del Mediterráneo se han relacionado con síntomas respiratorios agudos esporádicos y malestar general en las personas expuestas a los aerosoles marinos. Sin embargo, la relación directa entre las proliferaciones recurrentes de Ostreopsis y los problemas en la salud no ha sido claramente establecida. Con el fin de establecer esta conexión se realizó un estudio ecológico y epidemiológico conjunto en una playa afectada por dichos eventos. A lo largo de la proliferación, que se extendió desde finales de junio hasta finales de octubre de 2013, el 81% de la cohorte humana estudiada presentó al menos un síntoma relacionado con los potencialmente producidos por Ostreopsis. Paradójicamente, la mayoría de los efectos se produjeron durante un breve período de tiempo, a principios de agosto, coincidiendo con la transición de la fase de crecimiento exponencial de la proliferación a la fase estacionaria. A partir de agosto y hasta mediados de octubre, durante dicha fase estacionaria en que se mantuvieron concentraciones elevadas de O. cf. ovata, los síntomas fueron negligibles. Durante el período de tiempo con mayor afectación en la salud, no se observó un patrón claro en la componente de viento de mar hacia tierra. Nuestra hipótesis principal es que los compuestos irritantes presentes en el aerosol se producen durante una fase fisiológica particular de las células de Ostreopsis en un momento concreto de la proliferación.Palabras clave...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Establishing the link between Ostreopsis cf.ovata blooms and human health impacts using ecology and epidemiology

Vila¹,

Abós‐Herràndiz²,

Isern‐Fontanet³

et al. 2016

Sci. Mar.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Molecular analyses of aerosols collected at a northwestern Mediterranean beach confirmed the presence of O. cf. ovata cells (Casabianca et al, 2013); at the same time, ovatoxin-a (OVTX-a, a PLTX-like toxin) and trace levels of OVTX-b, d+e, and putative PLTX were detected . A recent simultaneous epidemiology and ecology study in an O. cf.…”

Section: Threats To Human Health and Well-being Posed By Bhabsmentioning

confidence: 99%

“…So far, real-time PCR (qPCR) assays exist for most of the Gambierdiscus species Nishimura et al, 2016) and for O. cf. ovata (Perini et al 2011;Casabianca et al, 2013). The availability of such assays applied to different environmental samples (i.e., macroalgae, water, aerosol, and other substrates) will be extremely helpful for timely monitoring of target cells during harmful events.…”

Section: Improving Taxonomy To Identify Organismsmentioning

confidence: 99%

Harmful Algal Blooms in Benthic Systems: Recent Progress and Future Research

Berdalet¹,

Tester²,

Chinain³

et al. 2017

Oceanog.

Self Cite

View full text Add to dashboard Cite

“…in marine aerosols (Casabianca et al, 2013) suggests that the occurrence of respiratory irritation and malaise related to aerosol exposure during Ostreopsis ovata blooms could be due to both the algal fragments and/or to the presence of Pltxs. Several cases of inhalation toxicity related to Ostreopsis proliferation occurred mainly in temperate climates, e.g., in the Mediterranean and Portuguese costs (Barroso García et al, 2008;Durando et al, 2007;Gallitelli et al, 2005;Sansoni et al, 2003;tichadou et al, 2010;tubaro et al, 2011b;Kermarec et al, 2008).…”

Section: Pectenotoxin Poisoning (Pep)mentioning

confidence: 99%

A roadmap for hazard monitoring and risk assessment of marine biotoxins on the basis of chemical and biological test systems

Daneshian

2013

ALTEX

View full text Add to dashboard Cite

Summary Aquatic food accounts for over 40% of global animal food products, and the potential contamination with toxins of algal origin -marine biotoxins -poses a health threat for consumers. The gold standards to assess toxins in aquatic food have traditionally been in vivo methodssingle marine biotoxins or combinations of marine biotoxins and intoxication symptomatology as well as monitoring the "success" of controls implemented during fishery and aquaculture production, processing, and retailing. For this reason, a workshop was organized in ermatingen, Switzerland by the Center for Alternatives to Animal testing -europe (CAAteurope) within the framework of the transatlantic think tank for toxicology (t 4 ).In contrast to other food products where hygiene and potential contamination with microbes or mold toxins is the prime issue, the emphasis in aquatic products (shellfish and finfish) is, beyond viral and bacterial contaminations, primarily placed on potential contamination with marine biotoxins owing to the numerous and recurring human intoxications.The gold standards to assess toxins in aquatic food have traditionally been in vivo methods, i.e., the mouse and the rat bioassays. Besides the ethical issues of in vivo bioassays there are specific difficulties, e.g., different exposure route (i.p. versus oral), low inter-species comparability, high intra-species variability, and questionable extrapolation of quantitative risk to humans, thus highlighting the need for the development of more reliable detection and quantification methods. Based on this reasoning, the european Food Safety Authority (eFSA) advocates the use of an analytical method, i.e., LC-MS (Liquid Chromatography coupled Mass Spectrometry), as a substitute for in vivo bioassays for almost all classes of marine toxins. However, although lC-MS is a very sensitive method that has the advantages of being able to detect multiple toxins in a single analysis and being good for confirming the identity of toxins, the quality of quantitative analysis is dependent on the availability of calibration standards. While many new toxin structural analogues have been detected and identified using LC-MS, this technique -as with most other methods -is not good at detecting new toxin types. When new toxin analogues are detected but accurate standards are not yet available, only an approximate quantitation is possible using estimated response factors.For the purpose of risk assessment of food, however, it is imperative to focus on the possible adverse effects, independent of whether they stem from one toxin or a combination of toxins. As toxicity is defined by functional and structural changes of biological systems, the development of a human-relevant in vitro system for appropriate risk assessment of marine biotoxins in seafood stands to reason. Moreover, poor correlation of human intoxications, of acute symptomatology and long-term adverse effects, and of predictive in vitro, in vivo, and analytical detection methodologies of marine biotoxins stems not least from a...

show abstract

Quantification of the Toxic Dinoflagellate Ostreopsis spp. by qPCR Assay in Marine Aerosol

Cited by 62 publications

References 37 publications

Establishing the link between <em>Ostreopsis</em> cf.<em>ovata</em> blooms and human health impacts using ecology and epidemiology

Establishing the link between <em>Ostreopsis</em> cf.<em>ovata</em> blooms and human health impacts using ecology and epidemiology

Harmful Algal Blooms in Benthic Systems: Recent Progress and Future Research

A roadmap for hazard monitoring and risk assessment of marine biotoxins on the basis of chemical and biological test systems

Contact Info

Product

Resources

About