Quantification of the mitochondrial DNA common deletion in presbycusis

Markaryan, Adam; Nelson, Erik G.; Hinojosa, Raúl

doi:10.1002/lary.20218

Cited by 87 publications

(63 citation statements)

References 19 publications

(28 reference statements)

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“…Recently, real-time PCR has been developed to investigate either mtDNA copy number or the percentage of common deletion in every subject [23] . A recent study which used real-time PCR to quantify the percentage of common deletion in archival temporal bone reported that presbycusis group had a mean common deletion level of 32% in archival temporal bone and normal hearing control group had only 12% in archival temporal bone [24] . Although the available evidence indicates that the common deletion is related to presbycusis, the specific role of this deletion in the generation of presbycusis remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Protective roles of α-lipoic acid in rat model of mitochondrial DNA4834bp deletion in inner ear

Peng

Zhong

et al. 2010

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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The protective roles of alpha-lipoic acid in the rat model of mitochondrial DNA (mtDNA) 4834bp deletion in inner ear were investigated. Forty female Wistar rats at 4 weeks of age were divided into four groups: group A (D-galactose group, n=10), group B (D-galactose+alpha-lipoic acid group, n=10), group C (alpha-lipoic acid group, n=10), and group D (control group, n=10). Auditory brainstem response (ABR) was used to detect the hearing threshold. Colorimetry was used to analyze activity of superoxide dismutase (SOD) and concentration of malondialdehyde (MDA). The percentage of mtDNA4834bp deletion in inner ear was identified by real-time PCR. There was no significant difference in ABR threshold shift among all groups. The percentage of mtDNA4834bp deletion in group A was higher than that in other groups, but there was no significant difference in percentage of mtDNA4834bp deletion among groups B, C, and D. The activity of SOD in group A was lower than that in other groups. The concentration of MDA in group A was higher than that in other groups. It was concluded that there was no significant hearing loss when the percentage of mtDNA4834bp deletion was lower than 12.5%. Alpha-lipoic acid could prevent the reactive oxygen species (ROS)-induced mtDNA4834bp deletion in inner ear of rats.

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Section: Discussionmentioning

confidence: 99%

Protective roles of α-lipoic acid in rat model of mitochondrial DNA4834bp deletion in inner ear

Peng

Zhong

et al. 2010

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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“…As compared with previous in vivo studies (3,15,26), analyzing mtDNA expression in peripheral blood and temporal bone specimens, the present study was non-invasive and effective through analysis of mtDNA CD4977 in the hair shaft. Therefore, hair shaft detection may be used to predict hearing function.…”

mentioning

confidence: 86%

“…Presbycusis is characterized by the progressive, bilaterally symmetrical, sensorineural and chronic loss of hearing. Although the mechanism of presbycusis is unclear, Markaryan A et al (3) demonstrated histopathologically, that degeneration of cochlea tissues, including the spiral ganglion, stria vascularis and hair cells, was linearly associated with hearing loss (3).…”

Section: Introductionmentioning

confidence: 99%

Association between the mitochondrial DNA 4977 common deletion in the hair shaft and hearing loss in presbycusis

Liu

Han

Wang

et al. 2014

Molecular Medicine Reports

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Abstract. The aim of the present study was to examine the role of the mitochondrial (mt) DNA common deletion (CD) 4977 (mtDNACD4977) in the hair shaft in patients with presbycusis. A total of 87 individuals with presbycusis and 95 normal-hearing controls were selected based on strict audiometric criteria. Nested polymerase chain reaction (PCR), sequencing and quantitative (q)PCR were used to examine the expression levels of mtDNA CD4977 in the hair shaft in presbycusis. Nested PCR of the hair shaft demonstrated that 8/95 cases with normal hearing were found to be positive for mtDNA CD4977 , as compared with 59/87 cases in the presbycusis group. The mtDNA CD4977 was positive in 22/43 cases with mild-to-moderate hearing loss, 25/31 cases with moderate-to-severe, severe hearing loss, and 12/13 cases with profound deafness. Statistically significant differences in mtDNA CD4977 expression were identified among all of the groups (P<0.001). The sequencing and qPCR assays demonstrated a trend towards an increase in the mean CD level of mtDNA CD4977 with a more severe hearing loss at 8 kHz (r=0.778, P<0.001) and all ranges of frequency (r=0.858, P<0.001). In conclusion, the present study demonstrates a correlation between mtDNA CD4977 in the human hair shaft and the severity of hearing loss in presbycusis.

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“…These observations were confi rmed in humans by Dai et al ( 2004 ), who found a high incidence of the mtDNA4977 deletion in the temporal bones of ARHL patients (17/34) compared to ears in the age-matched control group (4/19) and those in the young and middleaged control group (0/14). Perhaps most compelling, a quantitative correlation was established between the level of the mtDNA4977 deletion in human temporal bone samples from ARHL patients and the severity of the hearing loss (Markaryan et al 2009 ). These authors also observed a decrease in the expression of the mitochondrial cytochrome c oxidase subunit 3 (COX3) gene in SGNs and an increase in deletions different from the mtDNA common deletion in COX3-defi cient SGNs (Markaryan et al 2010 ).…”

Section: Mitochondrial Mutations and Dysfunction And Its Association mentioning

confidence: 99%

Cellular Mechanisms of Age-Related Hearing Loss

Melgar–Rojas

Alvarado

Fuentes–Santamaría

et al. 2015

Oxidative Stress in Applied Basic Research and Clinical Practice

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Quantification of the mitochondrial DNA common deletion in presbycusis

Abstract: For the first time, to our knowledge, these results demonstrate a relationship between quantitatively measured levels of the CD in human cochlear tissue and the severity of hearing loss in individuals with presbycusis. Laryngoscope, 2009.

Cited by 87 publications

References 19 publications

Protective roles of α-lipoic acid in rat model of mitochondrial DNA4834bp deletion in inner ear

Protective roles of α-lipoic acid in rat model of mitochondrial DNA4834bp deletion in inner ear

Association between the mitochondrial DNA 4977 common deletion in the hair shaft and hearing loss in presbycusis

Cellular Mechanisms of Age-Related Hearing Loss

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