Quantification of THC in Cannabis plants by fast-HPLC-DAD: A promising method for routine analyses

Burnier, Céline; Esseiva, Pierre; Roussel, Christophe

doi:10.1016/j.talanta.2018.09.012

Cited by 40 publications

(29 citation statements)

References 19 publications

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“…Various HPLC and UPLC methods that have been reported for the analysis of cannabinoids in C. sativa L. plant samples and Cannabis consumer products, e.g. hashish, marijuana and cannabis oils, since the year 2010, are summarised in Tables and , and appraised in the following subsections.…”

Section: Hplc and Uplc (Or Uhplc) Analysis Of Naturally Occurring Canmentioning

confidence: 99%

“…Both HPLC and UPLC methods have been applied to separate, identify and quantify various cannabinoids in C. sativa L. samples, including whole plants, roots, inflorescences and biomass containing Cannabis plant parts (Table ). However, the use of simple HPLC‐based methods has been observed more often than UPLC‐based methods for the analysis of C. sativa plant samples in the last decade.…”

Section: Hplc and Uplc (Or Uhplc) Analysis Of Naturally Occurring Canmentioning

confidence: 99%

“…Several HPLC and UPLC‐based methods for the analysis of various extracts of C. sativa whole plants have been reported . While the UV or UV‐PDA‐based detection is quite common, ESI‐MS/MS detection has been increasingly becoming the method of choice for cannabinoids analysis from C. sativa plant crude extracts by HPLC or UPLC.…”

Section: Hplc and Uplc (Or Uhplc) Analysis Of Naturally Occurring Canmentioning

confidence: 99%

“…The retention order (in increasing retention time) of these cannabinoids were CBDA ( 4 ) < CBGA ( 7 ) < CBG ( 6 ) < CBD ( 3 ) < CBN ( 8 ) < THC ( 12 ) < THCA ( 13 ). A very similar fast‐HPLC‐PDA method (detection at 211 nm) has recently been published, but instead of a water–ACN gradient, an isocratic elution at 35°C was used for the simultaneous detection of CBD ( 3 ), THC ( 12 ) and THCA ( 13 ) within a run time of only 5 min, establishing this method as a low cost alternative to UPLC for routine analyses of cannabinoids. During the past decade, it seems that only one UPLC‐UV based method for the detection of several cannabinoids in plant samples was reported, where the particle size of C 18 silica was of typical UPLC (or UHPLC) range, 1.7 μm (Table ).…”

Section: Hplc and Uplc (Or Uhplc) Analysis Of Naturally Occurring Canmentioning

confidence: 99%

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A review on the recent advances in HPLC, UHPLC and UPLC analyses of naturally occurring cannabinoids (2010–2019)

Nahar

Önder

Sarker

2019

Phytochemical Analysis

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Introduction Organic molecules that bind to cannabinoid receptors are called cannabinoids, and they have similar pharmacological properties like the plant, Cannabis sativa L. Hyphenated liquid chromatography (LC), incorporating high‐performance liquid chromatography (HPLC) and ultra‐performance liquid chromatography (UPLC, also known as ultrahigh‐performance liquid chromatography, UHPLC), usually coupled to an ultraviolet (UV), UV‐photodiode array (PDA) or mass spectrometry (MS) detector, has become a popular analytical tool for the analysis of naturally occurring cannabinoids in various matrices. Objective To review literature on the use of various LC‐based analytical methods for the analysis of naturally occurring cannabinoids published since 2010. Methodology A comprehensive literature search was performed utilising several databases, like Web of Knowledge, PubMed and Google Scholar, and other relevant published materials including published books. The keywords used, in various combinations, with cannabinoids being present in all combinations, in the search were Cannabis, hemp, cannabinoids, Cannabis sativa, marijuana, analysis, HPLC, UHPLC, UPLC, quantitative, qualitative and quality control. Results Since 2010, several LC methods for the analysis of naturally occurring cannabinoids have been reported. While simple HPLC‐UV or HPLC‐UV‐PDA‐based methods were common in cannabinoids analysis, HPLC‐MS, HPLC‐MS/MS, UPLC (or UHPLC)‐UV‐PDA, UPLC (or UHPLC)‐MS and UPLC (or UHPLC)‐MS/MS, were also used frequently. Applications of mathematical and computational models for optimisation of different protocols were observed, and pre‐analyses included various environmentally friendly extraction protocols. Conclusions LC‐based analysis of naturally occurring cannabinoids has dominated the cannabinoids analysis during the last 10 years, and UPLC and UHPLC methods have been shown to be superior to conventional HPLC methods.

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Section: Hplc and Uplc (Or Uhplc) Analysis Of Naturally Occurring Canmentioning

confidence: 99%

Section: Hplc and Uplc (Or Uhplc) Analysis Of Naturally Occurring Canmentioning

confidence: 99%

Section: Hplc and Uplc (Or Uhplc) Analysis Of Naturally Occurring Canmentioning

confidence: 99%

Section: Hplc and Uplc (Or Uhplc) Analysis Of Naturally Occurring Canmentioning

confidence: 99%

See 2 more Smart Citations

A review on the recent advances in HPLC, UHPLC and UPLC analyses of naturally occurring cannabinoids (2010–2019)

Nahar

Önder

Sarker

2019

Phytochemical Analysis

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“…Dentre os métodos por CLAE publicados, 12,14,17,19,20,21 Apesar do método selecionado 12 ter sido aplicado a amostras vegetais empregando coluna C18 com menor tamanho de partícula (2 µm) para melhor resolução dos picos, a coluna empregada neste trabalho com tamanho de partícula de 5 µm e o gradiente modificado mostraram-se adequados para a separação dos cinco canabinoides no IFAV e nos extratos formulados em matrizes oleosas conservando-se a sequência de eluição dos picos.…”

Section: Resultsunclassified

QUANTIFICAÇÃO DE CANABINOIDES EM EXTRATOS MEDICINAIS DE Cannabis POR CROMATOGRAFIA LÍQUIDA DE ALTA EFICIÊNCIA

Carvalho¹,

Aguiar²,

Baratto³

et al. 2020

Quím. Nova

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Recebido em 07/06/2019; aceito em 25/09/2019; publicado na web em 08/01/2020 CANNABINOIDS QUANTIFICATION IN MEDICINAL Cannabis EXTRACTS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. Cannabidiol (CBD) and tetrahydrocannabinol (THC) are the main active principles in the Cannabis extracts. Rich-CBD extracts are indicated to control seizures in epileptic patients, THC is indicated as antiemetic to patients in chemotherapy treatment and extracts with THC and CBD in proportions of approximately 1:1 are indicated to control spasticity in patients with multiple sclerosis. In Brazil epileptic pediatric patients are treated with imported Cannabis extracts registered as food supplement and with national not regulated Cannabis extracts. With the medical Cannabis sanitary regulation in Brazil, quality control methods will be required. A High Performance Liquid Chromatography (HPLC) method was developed and validated for five cannabinoids quantification in oily extracts and resins. The method was applied to 101 Cannabis extracts consumed by patients attended by a risk assessment program. Linearity, selectivity and precision met the validation criteria of national and international sanitary agencies. Cannabis imported oily extracts showed high CBD levels (72.53±60.63 mg mL-1) and low THC levels (1.21±1.32 mg mL-1), while national Cannabis extracts showed mainly two profiles, first composed by negative or cannabinoids traces and the second composed by THC rich extracts (9.81±13.49 mg mL-1). The HPLC method is useful to pharmaceutical production and risk assessment purposes.

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Analytical strategies for herbal Cannabis samples in forensic applications: A comprehensive review

Slosse

Durme

Eliaerts

et al. 2023

WIREs Forensic Science

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Cannabis sativa L. is undoubtedly the most used recreational drug worldwide because of its desired acute psychotropic effects, like relaxation, euphoria and altered perceptions. In addition, promising medical properties of Cannabis components have gained a lot of attention, resulting in a debate to permit recreational Cannabis use in several countries. In recent years, this controversial plant was increasingly studied and a large number of scientific papers were published. Herbal Cannabis consists of a variable and complex matrix, which makes it challenging to properly seize and prepare the sample for qualitative and quantitative analysis. Moreover, both the adoption of legal cut‐off values in different countries for the Δ9‐tetrahydrocannabinol (THC) content in seizures, and the emergence of cannabidiol (CBD) based products, containing generally small but variable amounts of THC, urged the need for sensitive and reliable analytical techniques to accurately identify and quantify the components of interest. This review presents detailed information on the procedure prior to analysis and covers chromatographic and spectroscopic methods developed for the analysis of cannabinoids in seizures for different forensic purposes, that is, identification/quantification, potency testing, drug‐ and fiber‐type differentiation, age estimation, yield determination and Cannabis profiling. Advantages and drawbacks of existing methods, within a specific forensic context, are discussed. The application of chemometrics, which offers a powerful tool in interpreting complex data, is also explained. This article is categorized under: Toxicology > Cannabis Toxicology > Drug Analysis Forensic Chemistry and Trace Evidence > Presentation and Evaluation of Forensic Science Output

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Quantification of THC in Cannabis plants by fast-HPLC-DAD: A promising method for routine analyses

Cited by 40 publications

References 19 publications

A review on the recent advances in HPLC, UHPLC and UPLC analyses of naturally occurring cannabinoids (2010–2019)

A review on the recent advances in HPLC, UHPLC and UPLC analyses of naturally occurring cannabinoids (2010–2019)

QUANTIFICAÇÃO DE CANABINOIDES EM EXTRATOS MEDICINAIS DE Cannabis POR CROMATOGRAFIA LÍQUIDA DE ALTA EFICIÊNCIA

Analytical strategies for herbal Cannabis samples in forensic applications: A comprehensive review

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