Quantification of sugars in wheat flours with an HPAEC-PAD method

Pico, Joana; Martínez, Mario M.; Martín, M.T.

doi:10.1016/j.foodchem.2014.10.103

Cited by 41 publications

(28 citation statements)

References 26 publications

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“…When higher concentrations were found, samples were previously diluted before being ianalysed. The within-run and the between-run precision of HPAEC-PAD analysis were all lower than 10% and are similar to those presented by Pico et al [6]. All of these parameters highlight the ability and the reliability of using HPAEC-PAD for the LoD, limit of detection; LoQ, limit of quantification; CV, coefficient of variation; GE, glucose equivalent; ME, maltose equivalent.…”

Section: Methods Validationsupporting

confidence: 84%

Characterization of saccharides released during an in vitro pepsin‐pancreatin digestion of corn flour using HPAEC‐PAD

et al. 2016

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Saccharides released after an in vitro pepsin‐pancreatin digestion of corn flour were quantified and characterized using high‐performance anion‐exchange chromatography coupled with a pulsed amperometric detector (HPAEC‐PAD) and two colorimetric approaches (glucose oxidase/peroxidase assay and the 3,5‐dinitrosalicylic acid assay). HPAEC‐PAD revealed five major saccharides as the result of in vitro pepsin‐pancreatin digestion of corn grain: glucose, isomaltose, maltose, maltotriose, and glucosyl‐maltotriose. Concentrations of glucose released as measured by the three methods assessed are similar when the in vitro pepsin‐pancreatin digestion is followed by post incubation with amyloglucosidase. This post incubation unfortunately leads to a loss of information about the degree of polymerization of oligosaccharides that can impact their absorption through intestinal tract. HPAEC‐PAD gives both qualitative and quantitative information and then seems more suitable for a nutritional appreciation of the digestion of starchy feedstuffs.

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Section: Methods Validationsupporting

confidence: 84%

Characterization of saccharides released during an in vitro pepsin‐pancreatin digestion of corn flour using HPAEC‐PAD

et al. 2016

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“…Another simple option suitable for determination of sugar class is high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). As in case of HPLC and CE, a derivatization step is not required, but experimental results proved that the technique is providing poor resolution for some components like sucrose (Pico et al 2015). Three different cyclitols extracted from alfalfa plant were identified using matrix-assisted laser desorption/ionization with time-offlight mass spectrometry (MALDI-TOF-MS); however, the technique is useful and sensitive for identification, but not suitable for quantification (Al-Suod et al 2018b).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Cyclitols and Sugars Following a Comprehensive Investigation of 40 Plants

et al. 2019

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Due to the important features of widely unexplored cyclitols, a comprehensive qualitative and quantitative study is needed. Moreover, measuring the possible available amounts of identified components in plant material represents a stringent need, due to their importance in phytomedicine and their use in food. The purpose of this study was to realize an extended investigation mainly of cyclitols, but of sugars and sugar alcohols as well, from natural sources. Thus, 17 target compounds (7 cyclitols and 2 sugar alcohols and 8 sugars) extracted from medicinal and edible plants are reported. All detected components were simultaneous separated in just one chromatographic run, using a single GC column. A number of 52 sources coming from 40 species were studied. Thus, we report 37 new sources of cyclitols. Moreover, almost for all cyclitols, the richest source was not investigated previously. Therefore, the obtained results can represent a valuable material for food, pharmaceutical, medical, or cosmetic industry interested in the use of cyclitols.

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“…Additionally, gas chromatography–mass spectrometry122,123 in which some mono- and disaccharides are derivatized for quantification is used; other methods include high-performance anion-exchange chromatography with pulsed amperometric detection,124,125 capillary electrophoresis, among others.…”

Section: Analytic Methods To Determine Intestinal Permeabilitymentioning

confidence: 99%

Intestinal permeability in a patient with liver cirrhosis

Valadez

Rivera-Espinosa

Méndez-Guerrero

et al. 2016

TCRM

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Liver cirrhosis is a worldwide public health problem, and patients with this disease are at high risk of developing complications, bacterial translocation from the intestinal lumen to the mesenteric nodes, and systemic circulation, resulting in the development of severe complications related to high mortality rate. The intestinal barrier is a structure with a physical and biochemical activity to maintain balance between the external environment, including bacteria and their products, and the internal environment. Patients with liver cirrhosis develop a series of alterations in different components of the intestinal barrier directly associated with the severity of liver disease that finally increased intestinal permeability. A “leaky gut” is an effect produced by damaged intestinal barrier; alterations in the function of tight junction proteins are related to bacterial translocation and their products. Instead, increasing serum proinflammatory cytokines and hemodynamics modification, which results in the appearance of complications of liver cirrhosis such as hepatic encephalopathy, variceal hemorrhage, bacterial spontaneous peritonitis, and hepatorenal syndrome. The intestinal microbiota plays a fundamental role in maintaining the proper function of the intestinal barrier; bacterial overgrowth and dysbiosis are two phenomena often present in people with liver cirrhosis favoring bacterial translocation. Increased intestinal permeability has an important role in the genesis of these complications, and treating it could be the base for prevention and partial treatment of these complications.

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Quantification of sugars in wheat flours with an HPAEC-PAD method

Cited by 41 publications

References 26 publications

Characterization of saccharides released during an in vitro pepsin‐pancreatin digestion of corn flour using HPAEC‐PAD

Characterization of saccharides released during an in vitro pepsin‐pancreatin digestion of corn flour using HPAEC‐PAD

Simultaneous Determination of Cyclitols and Sugars Following a Comprehensive Investigation of 40 Plants

Intestinal permeability in a patient with liver cirrhosis

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