Quantification of Stromal Thinning, Epithelial Thickness, and Corneal Haze after Photorefractive Keratectomy Using In Vivo Confocal Microscopy

Quantitative assessment of local collagen matrix remodeling in 3-D Culture: The role of Rho kinase

“…Corneal fibroblasts migrate into the wound, synthesize new extracellular matrix, and reorganize the ECM through the application of mechanical forces. Wound contraction and matrix remodeling by corneal fibroblasts ultimately determine a patient's corneal clarity and refractive outcome [47][48][49]. Understanding the underlying cellular and molecular mechanisms that regulate these biophysical processes in corneal fibroblasts could ultimately lead to more effective approaches to modulating the wound healing response in vivo.…”

Use of In Vivo Confocal Microscopy to Understand the Pathology of Accidental Ocular Irritation

“…In the normal cornea, CMTF allows representation of the surface epithelium, basal lamina, stromal keratocyte nuclei, and endothelium as an intensity profile (i.e., tracing) derived by calculating differences in pixel intensity from a scan of the entire cornea at a constant lens speed (15,20). Changes in intensity profiles can then be used to quantitate objectively the depth and thickness of subcomeal structures (15,20). Actual images taken during the scanning can be viewed to identify pathologic processes underlying the elevated intensities (15,20 (19).…”

“…Changes in intensity profiles can then be used to quantitate objectively the depth and thickness of subcomeal structures (15,20). Actual images taken during the scanning can be viewed to identify pathologic processes underlying the elevated intensities (15,20 (19). In the previous study, for the conjunctiva and cornea, extent of initial injury in 1 cohort of rats strongly correlated with responses in other cohorts of rats (19) In vivo CM has allowed us to provide data to support our premise that area and depth of injury are principal factors determining early response and eventually repair processes following accidental eye irritation (12,17).…”

Quantitative Characterization of Acid- and Alkali-Induced Corneal Injury in the Low-Volume Eye Test

“…In study 1 (4,5,8,13 (10,12,24). Video images were digitized using a Silicon Graphics workstation, and pixel-intensity profiles were generated to measure epithelial, stromal, and corneal thickness and to estimate back- 10% acetic acid, and 2% NaOH, but the MAS was decreased for 8% NaOH.…”