Quantification of Sodium Dodecyl Sulfate in Microliter-Volume Biochemical Samples by Visible Light Spectroscopy

Rusconi, Filippo; Valton, Édouard; Nguyen, Régis; Dufourc, Érick J.

doi:10.1006/abio.2001.5164

Cited by 72 publications

(60 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The quantiˆcation method for SLS with colorimetry using Stains-all dye was adopted from a previously reported study 5) and validated for linearity, precision, accuracy, and inter-and intraday variation as per the ICH guidelines. 6)`S tains-all'' stock solution (1.8 mM) was prepared and further diluted with formamide and deionized water to prepare Stains-all intermediate solution (this solution is stable for 3 days when stored in the dark at 4°C), which was used for standard curve preparation and sample analysis.…”

Section: Quantiˆcation Of Polymer and Surfactant In Crystals Sls Quanmentioning

confidence: 99%

Role of Additives like Polymers and Surfactants in the Crystallization of Mebendazole

2008

View full text Add to dashboard Cite

Crystallization in the presence of additives like surfactants and polymers is a relatively less explored area but is important for polymorphic screening of a compound during its developmental stage. Surfactants and polymers act by various mechanisms to in‰uence either the growth or the nucleation phase, resulting in modiˆcation of either the polymorphic form or the crystal habit. The present study was aimed at understanding the crystallization behavior of the model drug mebendazole (MBZ) in the presence of an inert polymer polyvinyl pyrrolidone (PVP) and an anionic surfactant sodium lauryl sulphate (SLS). Crystals were generated by the antisolvent approach using the surfactant and polymer solutions in water as the antisolvents. Change in habit from needles to plates took place as a result of modiˆca-tion of the crystallization process in the presence of additive molecules. This was conˆrmed by quantiˆcation of these additives using speciˆc analytical methods, which revealed their presence in small amounts in theˆnal product (0.02, 0.15, and 0.24％ w/w SLS in crystals generated using 0.5, 1.0, and 2.0％ SLS, respectively, and 0.94, and 1.24％ w/w PVP K30 and PVP K90, respectively). Their presence in the crystals led to modiˆcation in the dissolution of the drug. SLS improved the extent of dissolution while PVP had a negative impact and led to reduction in the amount of MBZ released even below that of the pure drug. The study highlights the in‰uence of polymeric and surfactant additives on the crystallization process leading to modiˆed performance.

show abstract

Section: Quantiˆcation Of Polymer and Surfactant In Crystals Sls Quanmentioning

confidence: 99%

Role of Additives like Polymers and Surfactants in the Crystallization of Mebendazole

2008

View full text Add to dashboard Cite

show abstract

“…Sodium dodecyl sulphate was quantified in culture supernatants with a modified Stains-all assay (Rusconi et al 2001). Nine hundred microlitres of an adequately diluted sample (triplicates) were mixed with 900 ll Stains-all assay solution and incubated for 2 min in the dark prior to reading the absorption at 438 nm wavelength.…”

Section: Sds Quantificationmentioning

confidence: 99%

Cell aggregation of Pseudomonas aeruginosa strain PAO1 as an energy-dependent stress response during growth with sodium dodecyl sulfate

et al. 2006

View full text Add to dashboard Cite

Pseudomonas aeruginosa strain PAO1 grew with the detergent sodium dodecyl sulfate (SDS). The growth started with the formation of macroscopic cell aggregates which consisted of respiring cells embedded in an extracellular matrix composed of acidic polysaccharides and DNA. Damaged and uncultivable cells accumulated in these aggregates compared to those cells that remained suspended. We investigated the response of suspended cells to SDS under different conditions. At high energy supply, the cells responded with a decrease in optical density and in viable counts, release of protein and DNA, and formation of macroscopic aggregates. This response was not observed if the energy supply was reduced by inhibiting respiration with KCN, or if cells not induced for SDS degradation were exposed to SDS. Exposure to SDS caused cell lysis without aggregation if cells were completely deprived of energy, either by applying anoxic conditions, by addition of CCCP, or by addition of KCN to a mutant defective in cyanideinsensitive respiration. Aggregated cells showed a more than 100-fold higher survival rate after exposure to SDS plus CCCP than suspended cells. Our results demonstrate that cell aggregation is an energy-dependent response of P. aeruginosa to detergent stress which might serve as a survival strategy during growth with SDS.

show abstract

“…5,7,8 Gilpin et al used colorimetric analysis to detect residual SDS present in tissue sampled from decellularized human and porcine lungs using the Stains-All solution. 5 The Stains-All solution was previously shown to be specific for SDS, among other potential contaminating ions and detergents, in a linear manner between 0% to 0.2% SDS, 14 while the general concentration of SDS used throughout lung decellularization protocols is 0.1-1%. 4,5,15 Increased detergent concentrations have been thought to be necessary for decellularizing lungs obtained from larger species and Triton X-100 has been used following SDS perfusion to remove residual SDS.…”

Section: Introductionmentioning

confidence: 99%

Residual Detergent Detection Method for Nondestructive Cytocompatibility Evaluation of Decellularized Whole Lung Scaffolds

Zvarova

Uhl

Uriarte

et al. 2016

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

The development of reliable tissue engineering methods using decellularized cadaveric or donor lungs could potentially provide a new source of lung tissue. The vast majority of current lung decellularization protocols are detergent based and incompletely removed residual detergents may have a deleterious impact on subsequent scaffold recellularization. Detergent removal and quality control measures that rigorously and reliably confirm removal, ideally utilizing nondestructive methods, are thus critical for generating optimal acellular scaffolds suitable for potential clinical translation. Using a modified and optimized version of a methylene blue-based detergent assay, we developed a straightforward, noninvasive method for easily and reliably detecting two of the most commonly utilized anionic detergents, sodium deoxycholate (SDC) and sodium dodecyl sulfate (SDS), in lung decellularization effluents. In parallel studies, we sought to determine the threshold of detergent concentration that was cytotoxic using four different representative human cell types utilized in the study of lung recellularization: human bronchial epithelial cells, human pulmonary vascular endothelial cells (CBF12), human lung fibroblasts, and human mesenchymal stem cells. Notably, different cells have varying thresholds for either SDC or SDS-based detergent-induced cytotoxicity. These studies demonstrate the importance of reliably removing residual detergents and argue that multiple cell lines should be tested in cytocompatibilitybased assessments of acellular scaffolds. The detergent detection assay presented here is a useful nondestructive tool for assessing detergent removal in potential decellularization schemes or for use as a potential endpoint in future clinical schemes, generating acellular lungs using anionic detergent-based decellularization protocols.

show abstract

Quantification of Sodium Dodecyl Sulfate in Microliter-Volume Biochemical Samples by Visible Light Spectroscopy

Cited by 72 publications

References 22 publications

Role of Additives like Polymers and Surfactants in the Crystallization of Mebendazole

Role of Additives like Polymers and Surfactants in the Crystallization of Mebendazole

Cell aggregation of Pseudomonas aeruginosa strain PAO1 as an energy-dependent stress response during growth with sodium dodecyl sulfate

Residual Detergent Detection Method for Nondestructive Cytocompatibility Evaluation of Decellularized Whole Lung Scaffolds

Contact Info

Product

Resources

About