Quantification of Sedimented Triamcinolone for Intravitreal Injection

Abstract

The authors have developed a simple protocol to use sedimentation to greatly increase the concentration of triamcinolone acetonide, starting from commercially available triamcinolone acetonide up to a maximum of 25.7 mg per 0.1 mL (257 +/- 9 mg/mL). This study demonstrates a practical and quantifiable method to increase triamcinolone concentration for intravitreal injections.

The Duration of Effect of Centrifuge Concentrated Intravitreal Triamcinolone Acetonide

“…They measured an average of 19.8 mg/0.1 mL (±1.1 mg/0.1 mL) after 30 minutes and 20.12 mg/0.1 mL (±0.9 mg/0.1 mL) after 40 minutes. Tsong et al 19 also described a technique to concentrate TA for intravitreal injection using sedimentation to a maximum of 25.7 mg/0.1 mL after 2 hours. Both of these studies independently concluded that shorter sedimentation times yielded lower concentrations.…”

An ultralow-cost portable centrifuge from discarded materials for medical applications

“…Owing to its generally lower cost, more generous reimbursement, and greater availability, attempts have been made to prolong the effects of intravitreal TA 1 , 3 , 16 , 17 . Given its low solubility in water, TA remains as a depot within the eye, producing gradual and relatively constant diffusion of the drug; thus, if a larger depot is injected, the longer the effect is expected to persist 1 , 3 .…”

“…To this end, several methods have been devised to concentrate TA suspensions prior to injection into the vitreous. While techniques relying on passive (i.e., gravity-dependent) sedimentation or Millipore filtration have been described, these approaches are relatively time-intensive and yield variable results [15][16][17] . In contrast, prior studies have demonstrated rapid and reliable concentration of TA (with a resulting prolongation of drug effect) through centrifugation-assisted sedimentation 1,3 .…”