Quantification of santonin in eight species of Artemisia from Kazakhstan by means of HPLC-UV: Method development and validation

Abstract

Santonin, a powerful anthelmintic drug that was formely used to treat worms, is Artemisia cina's main constituent. However, due to its toxicity to humans, it is no longer in use. Kazakhstan is looking to introduce this plant as an anthelmintic drug for veterinary purposes, despite the known toxic properties of the santonin. The objective of this study was to develop a fast and specific method for the identification of santonin and its precise quantitation using HPLC-UV in order to avoid unnecessary intoxication, which is paramount for the development of veterinary medicines. The results obtained showed that santonin appears at around 5.7 minutes in this very reliable HPLC method. The validation of the method was performed by the investigation of parameters such as precision, accuracy, reproducibility and recovery. The method was used to identify and quantify santonin in leaves of A. scoparia, A. foetida, A. gmelinni, A. schrenkiana, A. frigida, A. sublesingiana, A terra-albae, and A. absinthium from Kazakhstan as well as in three different extracts of leaves of A. cina. This study has provided a faster and simpler method for the identification and quantification of this compound in other species of Artemisia of economic importance.

Chemical composition and enzyme inhibition of Phytolacca dioica L. seeds extracts

“…Compound 1 : Mixture of Isoamericanol B1, B2, C1, and C2 UV (MeOH) λ max nm: 334; HRESI–MS m/z [M − H]+: 493 (related to isoamericanol C1 and C2); 1 H NMR (Methanol-D4, 400 MHz) 28 (Table 4).…”

“…The purity of fractions obtained by HSCCC was determined by TLC analysis and HPLC-DAD-ESI/MS analyses and the compounds were identified by MS and 1H NMR analysis 28 .…”

Chemistry and Pharmacology of the Kazakh Crataegus Almaatensis Pojark: An Asian Herbal Medicine

“…The HPLC analysis showed a retention time of approximately 15.2 min for afzelin, 13.4 min for quercitrin, and 11.7 min for hyperoside (Figure 2). The calibration curves were based on the method described and validated by Sakipova et al [25] and all substances showed good linearity with regression coefficients R 2 ≥ 0.99 [26]. The calibration curves were used to quantify the substances in the total extract using the CECa chromatogram at a concentration of 1250 µg/mL.…”

Research Advances on Health Effects of Edible Artemisia Species and Some Sesquiterpene Lactones Constituents

“…Santonin is the most abundant sesquiterpene lactone in Artemisia cina Berg ex Poljakov and was isolated from several Artemisia species [ 254 , 255 , 256 ], including some edible species such as A. absinthium , A. frigida , A. tridentata [ 34 , 255 ]. Artemisia santonicum is commonly referred to as “wormseed”, a name historically attributed to the plants’ anthelmintic (“worms”) activity [ 251 ].…”