Quantification of saffron (Crocus sativus L.) metabolites crocins, picrocrocin and safranal for quality determination of the spice grown under different environmental Moroccan conditions

Lage, M.; Cantrell, Charles L.

doi:10.1016/j.scienta.2009.02.017

Cited by 215 publications

(159 citation statements)

References 31 publications

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“…Picrocrocin and safranal concentrations were comparable to those formerly described for Crocus stigmas [16]. Data on concentrations of individual crocetin glycosides (tCdG and tCGG) can be found in [9] and [25], the concentrations of these chief CGs were comparable to those measured in this work.…”

Section: Testing Of the Methodssupporting

confidence: 89%

“…The proposed method can be an inexpensive means of screening and quantification. It can be a complementary analytical procedure to the published HPLC methods [16] and [19], as it allows quantification of all main constituents (picrocrocin, the two main crocetin glycosides: tCdG and tCGG, and safranal) within reasonable time, from a single stigma of the Crocus sativus plant. The proposed method can thus be used for plant-to-plant variability studies.…”

Section: Testing Of the Methodsmentioning

confidence: 99%

“…CGs and picrocrocin were purified using a protocol based on [16]. To purify the CGs, the plant material was extracted with MeOH exhaustively, filtered, evaporated to dryness, reuptaken in 10% MeCN, and separated to fractions by RP-HPLC -Shimadzu SPD-M10A HPLC, semipreparative column: Supelco Supelcosil LC-18 column (25 cm × 10 mm, 5 μm), flow rate 3 ml/min, using a water-MeCN linear gradient (from 25 to 100%, 60 min, +0.1% TFA).…”

Section: Purification Of Pircocrocin and Crocetin Glycosides From Safmentioning

confidence: 99%

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Quantification of main bioactive metabolites from saffron (Crocus sativus) stigmas by a micellar electrokinetic chromatographic (MEKC) method

Gonda

Parizsa

Surányi

et al. 2012

Journal of Pharmaceutical and Biomedical Analysis

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Quantification of main bioactive metabolites from saffron (Crocus sativus) stigmas by a micellar electrokinetic chromatographic (MEKC) AbstractSaffron is an expensive spice, cultivated in many regions of the world. Its chief metabolites include crocins, which are responsible for the coloring ability, safranal, which is the main essential oil constituent, and picrocrocin which is the main bitter constituent of the spice. A simple micellar capillary electrochromatographic (MEKC) method capable of quantifying all three types of main constituents was established. The pH, sodium dodecyl sulphate (SDS) content and electrolyte concentration of the background electrolyte was optimized. A simple extraction protocol was developed which can extract all metabolites of different polarity from the saffron stigmas. Optimal background electrolyte composed of 20 mM disodium phosphate, 5 mM sodium tetraborate, 100 mM SDS, pH was set 9.5. Optimal extracting solvent was the background electrolyte, incubated with the sample for 60 min. The proposed method allows quantification of picrocrocin, safranal, crocetin-Di-(β-dgentiobiosyl) ester and crocetin (β-d-glycosyl)-(β-d-gentiobiosyl) ester within 17.5 min, with limit of detection values ranging from 0.006 to 0.04 mg/ml, from a single stigma.

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Section: Testing Of the Methodssupporting

confidence: 89%

Section: Testing Of the Methodsmentioning

confidence: 99%

Section: Purification Of Pircocrocin and Crocetin Glycosides From Safmentioning

confidence: 99%

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Quantification of main bioactive metabolites from saffron (Crocus sativus) stigmas by a micellar electrokinetic chromatographic (MEKC) method

Gonda

Parizsa

Surányi

et al. 2012

Journal of Pharmaceutical and Biomedical Analysis

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“…12 In addition to these compounds, saffron also contains little amounts of other pigments like anthocyanin, α-carotene, β-carotene, and zegxantin. 13 Possible mechanism of action of saffron and its ingredient in diseases prevention…”

Section: Chief Ingredients Of Saffronmentioning

confidence: 99%

“…Stigma ethanolic fraction of saffron showed the highest antioxidant activity that might be recognized due to the presence of phenolics and flavonoids. [3][4][5][6][7][8][9][10][11][12][13][14] Other experimental data advocates that saffron helps in reduction of inflammation through the inhibition of cycoloxygenase enzyme activity. Saffron alleviates neuropathic pain via reduction of proinflammatory cytokines, antioxidant activity and apoptotic pathways 15 and in vitro study has revealed that crocin possess dual inhibitory activity against COX-1 and COX-2 enzymes.…”

Section: Chief Ingredients Of Saffronmentioning

confidence: 99%

Saffron (Crocus sativus) and its Active Ingredients: Role in the Prevention and Treatment of Disease

Rahmani¹,

Khan²,

Aldebasi³

2017

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Introduction:Crocus sativus is a member of iridaceae family and its use in health management is in practice since ancient time. Additionally, research based on clinical trials and animal models have advocates its role in disease cure without any severe adverse complications. The aim of this study is to summarize the study of saffron and its ingredients based on in vivo and in vitro and role in disease cure and prevention. Materials: This study was designed through the search engines such as Pub Med, Scopus and Google Scholar. The keywords used for the search were mainly focused on Saffron with health benefit and its therapeutics role in different diseases. Result: Saffron stigma is mixture of various components and recent studies have proven that saffron and its active ingredients play a key role in disease management. Moreover, clinical trials based study on the use of saffron and its individual components have confirmed the health promising effects. Conclusion: Although Saffron has potential role in the disease cure and prevention via modulation of anti-oxidant, anti-inflammatory, anti-tumour, anti-microbial and anti-diabetic activity. Furthermore, advanced research is needed to elaborate the role of saffron in health management and its mechanism of action in the modulation of biological activities.

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Detection of Food Adulterants in Different Foodstuff

Negi¹,

Lakshmi²,

Praba³

et al. 2021

Food Chemistry

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Quantification of saffron (Crocus sativus L.) metabolites crocins, picrocrocin and safranal for quality determination of the spice grown under different environmental Moroccan conditions

Cited by 215 publications

References 31 publications

Quantification of main bioactive metabolites from saffron (Crocus sativus) stigmas by a micellar electrokinetic chromatographic (MEKC) method

Quantification of main bioactive metabolites from saffron (Crocus sativus) stigmas by a micellar electrokinetic chromatographic (MEKC) method

Saffron (Crocus sativus) and its Active Ingredients: Role in the Prevention and Treatment of Disease

Detection of Food Adulterants in Different Foodstuff

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