Quantification of RNA in bacteriophage MS2-like viruses in solution by small-angle X-ray scattering

Kuzmanovic, Deborah A.; Elashvili, Ilya; Wick, Charles H.; O’Connell, Catherine M.; Krueger, Susan

doi:10.1016/j.radphyschem.2005.11.005

Cited by 9 publications

(19 citation statements)

References 37 publications

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“…MS2 phage were grown and purified by ultracentrifugation as described. 38 RNA-free MS2 capsids were produced as described, 39 using purified WT MS2 virus as starting material.…”

Section: Methodsmentioning

confidence: 99%

“…This result is in agreement with that obtained from SAXS measurements of MS2-capsid. 39 In summary, the core-shell model describes the shape of the structures formed in solution by the wild-type MS2 virus and its empty MS2 capsid. Using the core-shell model, the dimensions of the wild-type MS2 and empty MS2-capsid protein shells (in solution) are found to be virtually indistinguishable at the resolution of SANS.…”

Section: Rna Content Does Not Affect the Packing Arrangement Of The Mmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…29,36,37 Furthermore, a recent experimental analysis of the wild-type MS2 virus using SANS and small-angle X-ray scattering (SAXS) has been reported, and a model of its physical structure in solution has been described. 38,39 To better understand the influence of RNA and the A protein on protein shell arrangement in vivo, we present the use of SANS and the contrast variation technique to solve the structures in solution of the (coat) protein shell component of two types of MS2 viruses: (a) wild-type MS2 (WT MS2) containing coat protein, A protein and RNA; and (b) two recombinant forms of MS2 virus lacking the A protein but containing various amounts of RNA, (MS2-HCV and MS2-l). In addition, empty capsids (MS2-capsid), containing coat protein and A protein, but lacking RNA, were measured for comparison to the protein shell structures of the above-mentioned samples.…”

Section: Introductionmentioning

confidence: 99%

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The MS2 Coat Protein Shell is Likely Assembled Under Tension: A Novel Role for the MS2 Bacteriophage A Protein as Revealed by Small-angle Neutron Scattering

Kuzmanovic

Elashvili²,

Wick³

et al. 2006

Journal of Molecular Biology

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“…MS2 phage were grown and purified by ultracentrifugation as described. 38 RNA-free MS2 capsids were produced as described, 39 using purified WT MS2 virus as starting material.…”

Section: Methodsmentioning

confidence: 99%

Section: Rna Content Does Not Affect the Packing Arrangement Of The Mmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The MS2 Coat Protein Shell is Likely Assembled Under Tension: A Novel Role for the MS2 Bacteriophage A Protein as Revealed by Small-angle Neutron Scattering

Kuzmanovic

Elashvili²,

Wick³

et al. 2006

Journal of Molecular Biology

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“…et al 1997). It has an icosahedron shape with a mean diameter of approximately 26 nm (Kuzmanovic et al 2006 …”

Section: Model Waters and Bacteriophagesmentioning

confidence: 99%

Virus Removal’ by Low Pressure Membranes in Wastewater Treatment: Effects of Ion Composition and Effluent Organic Matter

Jacangelo¹,

Huang²,

Young³

et al. 2008

proc water environ fed

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Low pressure membranes (LPMs) have been increasingly applied in the disinfection of wastewater effluent. The efficiency of LPM in disinfection is affected by the quality of the effluent, including ion composition and effluent organic matter (EfOM). In order to better understand the two effects, two types of commercial LPMs with similar pore size rating were challenged with MS2 bacteriophage in this study at bench and pilot scales. It was found that ion composition had different effects on the performance of LPMs with loose and tight pore structures, probably as a result of different role of electrostatic repulsions between viruses and the membranes. Meanwhile, the EfOM effect was related to the presence of a colloidal fraction larger than other dissolved organics. Detailed mechanism for the EfOM effect has not been identified.The results indicate that the performance of LPMs in wastewater disinfection may be strongly influenced by the effluent ion composition and EfOM properties and different from that determined in standard bench-scale challenge tests. This should be carefully considered in the evaluation and selection of LPMs for full-scale applications.

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Label‐Free Analysis of Single Viruses with a Resolution Comparable to That of Electron Microscopy and the Throughput of Flow Cytometry

Zhu

Tian

et al. 2016

Angewandte Chemie

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Viruses are by far the most abundant biological entities on our planet, yet existing characterization methods are limited by either their speed or lack of resolution. By applying alaboratory-built high-sensitivity flowcytometer (HSFCM) to precisely quantify the extremely weak elastically scattered light from single viral particles,w eh erein report the label-free analysis of viruses with ar esolution comparable to that of electron microscopyand the throughput of flowcytometry.The detection of single viruses with diameters down to 27 nm is described. T7 and lambda bacteriophages,whichdiffer in size by as little as 4nm, could be baseline-resolved. Moreover, subtle structural differences of the same viral particles can be discriminated. Using monodisperse silica nanoparticles as the sizer eference standards,t he virus sizes measured by the HSFCM are in agreement with the equivalent particle diameters derived from their structural dimensions.T he HSFCM opens anew avenue for virus characterization.Viruses are by far the most abundant biological entities on our planet. [1] Whereas animal viruses are notoriously responsible for alarge number of fatal diseases,plant viral pathogens cause significant losses in crop yields worldwide each year.On the other hand, the high transfection efficiency of viral genetherapy vectors and their monodisperse structures with precise shapes and sizes make viral particles powerful drugdelivery vehicles and versatile nanotechnology building blocks. [2][3][4] Therefore,t he high-resolution and high-throughput analysis of single viral particles is of great importance for virology research, disease diagnosis and treatment, and biotechnology and nanotechnology applications.Tr ansmission electron microscopy (TEM) has historically been the method of choice for determining the size and morphology of single viruses.However, the tedious processes required for sample preparation and image analysis,a long with the high cost, prevent its use on aroutine basis.Recently, many newly developed single-particle techniques have been applied to single-virus analysis,s uch as surface plasmon resonance imaging, [5] whispering-gallery-mode microcavities, [6] suspended micro-and nanochannel resonators, [7] scanning probe microscopy, [8] resistive pulse sensing with nanopores, [9] and nanoparticle tracking analysis (NTA). [10] Although some of these techniques have proven to be very useful in various fields,as imple and practical approach with exceptional sensitivity and high resolution remains to be developed. Ther eal-time,l abel-free analysis of the size and composition of individual viral particles could be enabled by the quantification of their elastically scattered light intensity. However,t he sixth-power dependence of the scattered light intensity on the particle size and the low dielectric contrast with the surrounding medium make it extremely challenging to discriminate single viruses over background scattering. Recently,Manoharan et al. reported the label-free tracking of individual cowpea chlorotic mott...

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Quantification of RNA in bacteriophage MS2-like viruses in solution by small-angle X-ray scattering

Cited by 9 publications

References 37 publications

The MS2 Coat Protein Shell is Likely Assembled Under Tension: A Novel Role for the MS2 Bacteriophage A Protein as Revealed by Small-angle Neutron Scattering

The MS2 Coat Protein Shell is Likely Assembled Under Tension: A Novel Role for the MS2 Bacteriophage A Protein as Revealed by Small-angle Neutron Scattering

Virus Removal’ by Low Pressure Membranes in Wastewater Treatment: Effects of Ion Composition and Effluent Organic Matter

Label‐Free Analysis of Single Viruses with a Resolution Comparable to That of Electron Microscopy and the Throughput of Flow Cytometry

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