Quantification of Reducing Sugars Based on the Qualitative Technique of Benedict

López, Alejandro Hernández; Félix, Daniel A Sánchez; Sierra, Zenaida Zuñiga; Bravo, Itzel García; Dinkova, Tzvetanka D.; Alejandre, Alma Xóchil Ávila

doi:10.1021/acsomega.0c04467

Cited by 35 publications

(23 citation statements)

References 12 publications

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“…(3), it was observed that the optimum wavelength was 740 nm for all the seven different samples tested. A similar result was reported by [16], who tested a wide range of wavelengths (490-890 nm) and also reported 740 nm as the optimum wavelength for Benedict's reagent. Fig.…”

Section: Resultssupporting

confidence: 86%

The Impact of Enzymatic Hydrolysis of Sewage Sludge as a Pre-treatment for Dark Fermentation

Al-Haddad¹,

Okoro-Shekwaga²,

Fletcher³

et al. 2022

IJESD

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For many years, sewage sludge has been processed for methane production in anaerobic digestion reactors at wastewater treatment plants around the world. Sewage sludge is produced in large quantities and is rich in biodegradable organic materials, from which sugars (e.g., glucose) can be produced, recovered and used as a substrate to support hydrogen production through the Dark Fermentation (DF) process. DF is one of several methods used for bio-hydrogen production, whereby fermentative bacteria are used to hydrolyse organic substrates to produce hydrogen gas. Carbohydrates (sugars) is one of the main fermentable substrates for hydrogen production, and they are considered the most favourable substrate for fermentative bacteria (e.g., Clostridium bacteria). Although sewage sludge is rich in organic materials, still the complexity of its structure and low carbon/nitrogen ratio limits the bio-hydrogen production via DF processes. Therefore, this paper addresses the impact of Enzymatic Hydrolysis (EH) as a pre-treatment of sewage sludge on enhancing the biodegradability and glucose content in sewage sludge. The result shows that using the EH process as pre-treatment for sewage sludge, enhanced the glucose content in sewage sludge and converted some of the macro sewage flocs to easy digestible micro flocs (glucose). Therefore, the substrate being more favourable and easier to digest by bacteria in the DF reactor, enhanced the production of hydrogen and VFAs. More research needs to be done to find the optimum enzyme dosage, initial substrate concentration and operation temperature (especially when the enzyme is used inside the DF reactor).

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Section: Resultssupporting

confidence: 86%

The Impact of Enzymatic Hydrolysis of Sewage Sludge as a Pre-treatment for Dark Fermentation

Al-Haddad¹,

Okoro-Shekwaga²,

Fletcher³

et al. 2022

IJESD

View full text Add to dashboard Cite

show abstract

“…Seven-day-old R. dentatus seedlings were used for the extraction and determination of carbohydrates as reducing sugar (Hernández-López et al, 2020), soluble protein (Singh et al, 2015), and total amino acids (Lee et al, 2013).…”

Section: Determination Of Primary Metabolitesmentioning

confidence: 99%

Phytochemistry, allelopathy and anticancer potentiality of Withania somnifera (L.) Dunal (Solanaceae)

Ahmed

El-Darier

2024

Braz. J. Biol.

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Withania somnifera is a wild plant that shows great activity and safety against several human diseases. The current research explored the plant's chemical composition and allelopathic effects on Rumex dentatus (recipient plant). Moreover, anticancer activity is also tested against four types of human cancer cell lines. Chemical analysis of W. somnifera showed a high percentage of saponins and tannins, while glycosides, alkaloids, and flavonoids occurred in the second order. Results of the allelopathic experiments revealed significant inhibition of the R. dentatus plumule and radicle lengths as well as their relative dry weights. In addition, significant reductions in some primary metabolites of R. dentatus, like non-reducing and total sugar as well as soluble proteins, were determined. Cytotoxic potentiality of W. somnifera was also proved against four different cancer lines, namely; human hepatocellular carcinoma cell line (HepG2), human non-small cell lung cancer cell line (A549), human breast cancer cell line (MCF7), and colon cancer cell line (CaCo2) with IC50 value of about 38, 19, 27, and 24 ��g/ml, respectively.

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“…Benedict’s reagent used for spectrophotometric measurements contains copper(II) sulfate, sodium citrate and sodium carbonate only . The free carbonyl group of the glucose molecule reduces the copper ion followed by precipitation of copper(I) oxide …”

Section: Approachmentioning

confidence: 99%

Introducing Second Year Analytical Chemistry Students to Research through Experimental Design in the Undergraduate Teaching Laboratory

Doughan

Shahmuradyan

2022

J. Chem. Educ.

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Advancements in technology have led to significant changes in the workforce and created a demand for expertise in science, technology, engineering, and mathematics (STEM). In response, curricula have evolved to emphasize essential skills such as critical thinking and communication to prepare students for STEM-based careers. To this end, postsecondary institutions continue to develop and incorporate project-based learning (PBL), course-based undergraduate research experiences (CUREs), and process-oriented guided-inquiry learning (POGIL). These opportunities can be offered in junior level (1st and 2nd year) courses by designing projects that match students' skills and knowledge. Opportunities at the junior level reach a larger student population and can increase interest in STEM-based careers. In this article, we introduce a project-based activity for the second-year analytical chemistry laboratory in which students design and conduct experiments to quantify analytes in real-life samples. Analytes selected for this project (acids or bases, ascorbic acid, betacarotene, calcium, oxalate, reducing sugars and starch) can be quantified using techniques familiar to second year students including titration and absorbance spectroscopy. Students first designed experimental procedures and received feedback before conducting the experiments. Each experiment was performed over two laboratory periods, which allowed students to modify procedures between iterations to improve their experimental design. This experience allowed students to develop 21st century competencies including critical thinking and problem solving, innovation, creativity and entrepreneurship, self-directed learning, collaboration, and communication.

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Quantification of Reducing Sugars Based on the Qualitative Technique of Benedict

Cited by 35 publications

References 12 publications

The Impact of Enzymatic Hydrolysis of Sewage Sludge as a Pre-treatment for Dark Fermentation

The Impact of Enzymatic Hydrolysis of Sewage Sludge as a Pre-treatment for Dark Fermentation

Phytochemistry, allelopathy and anticancer potentiality of Withania somnifera (L.) Dunal (Solanaceae)

Introducing Second Year Analytical Chemistry Students to Research through Experimental Design in the Undergraduate Teaching Laboratory

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