Quantification of recombinant human relaxin-2 (B-29/A-24) in non-pregnant rat plasma using ultra performance liquid chromatography-mass spectrometry

Zhu, Xiaoyan; Liu, Jingjing; Wu, Jie; Cao, Ruibing; Li, Taiming

doi:10.1039/c3ay42194e

Anal. Methods

2014

DOI: 10.1039/c3ay42194e

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Quantification of recombinant human relaxin-2 (B-29/A-24) in non-pregnant rat plasma using ultra performance liquid chromatography-mass spectrometry

Xiaoyan Zhu

Jingjing Liu

Jie Wu

et al.

Abstract: A sensitive analytical method using ultra performance liquid chromatography-tandem mass spectrometry is developed for the determination of recombinant human relaxin-2 (B-29/A-24) in rats for characterizing pharmacokinetics.

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Cited by 9 publications

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A validated UPLC-MS/MS method coupled with protein precipitation and ion exchange solid phase extraction for the quantitation of porcine relaxin B29 in dog plasma and its application to a pharmacokinetic study

Sun

Yang

et al. 2017

Anal Bioanal Chem

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Porcine relaxin is a 6 kDa peptide hormone of pregnancy with important physiological and pharmacological effects. It contains a number of analogs of which porcine relaxin B29 is one of the most important. To support the development of porcine relaxin B29 as a new drug, we established an UPLC-MS/MS method for its quantitation in dog plasma. Sample preparation by protein precipitation and ion exchange solid phase extraction was followed by UPLC on an XBridge™ BEH300 C18 column at 40 °C in a run time of only 5.5 min. Detection was performed on a Qtrap 6500 mass spectrometer using ESI in the positive ion mode with MRM of the transitions at m/z 831.7 [M+7H] → 505.4 and m/z 1162.4 [M+5H] → 226 for pRLX B29 and internal standard (recombinant human insulin), respectively. The method was linear over the concentration range 30-2000 ng/mL with no matrix effects. Intra- and inter-day precisions were < 15% with accuracies in the range 98.8-100.6%. The method was successfully applied to a pharmacokinetic study in beagle dogs after administration of a 0.15 mg/kg intravenous dose. Graphical abstract Sample preparation and detection procedure.

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A validated UPLC-MS/MS method coupled with protein precipitation and ion exchange solid phase extraction for the quantitation of porcine relaxin B29 in dog plasma and its application to a pharmacokinetic study

Sun

Yang

et al. 2017

Anal Bioanal Chem

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Quantitation of a PEGylated protein in monkey serum by UHPLC-HRMS using a surrogate disulfide-containing peptide: A new approach to bioanalysis and in vivo stability evaluation of disulfide-rich protein therapeutics

Zheng

Zeng

Manney

et al. 2016

Analytica Chimica Acta

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Post-extraction disulfide bond cleavage for MS/MS quantification of collision-induced dissociation-resistant cystine-cyclized peptides and its application to the ultra-sensitive UPLC-MS/MS bioanalysis of octreotide in plasma

Sauter

Uhl

Burhenne

et al. 2020

Analytica Chimica Acta

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Quantification of recombinant human relaxin-2 (B-29/A-24) in non-pregnant rat plasma using ultra performance liquid chromatography-mass spectrometry

Abstract: A sensitive analytical method using ultra performance liquid chromatography-tandem mass spectrometry is developed for the determination of recombinant human relaxin-2 (B-29/A-24) in rats for characterizing pharmacokinetics.

Cited by 9 publications

References 16 publications

A validated UPLC-MS/MS method coupled with protein precipitation and ion exchange solid phase extraction for the quantitation of porcine relaxin B29 in dog plasma and its application to a pharmacokinetic study

A validated UPLC-MS/MS method coupled with protein precipitation and ion exchange solid phase extraction for the quantitation of porcine relaxin B29 in dog plasma and its application to a pharmacokinetic study

Quantitation of a PEGylated protein in monkey serum by UHPLC-HRMS using a surrogate disulfide-containing peptide: A new approach to bioanalysis and in vivo stability evaluation of disulfide-rich protein therapeutics

Post-extraction disulfide bond cleavage for MS/MS quantification of collision-induced dissociation-resistant cystine-cyclized peptides and its application to the ultra-sensitive UPLC-MS/MS bioanalysis of octreotide in plasma

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