Quantification of Proteins in Dairy Products Using an Optical Biosensor

Dupont, Didier; Muller-Renaud, Stéphanie

doi:10.1093/jaoac/89.3.843

Cited by 21 publications

(10 citation statements)

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“…Simultaneous detection of three caseins in milk using an SPR method was demonstrated by Dupont and Muller-Renaud . They used the commercial SPR sensor platform Biacore 3000 and sandwich assay format employing two monoclonal antibodies directed against the N- and C-terminal extremities of each of the caseins, respectively.…”

Section: 28 Proteinsmentioning

confidence: 99%

Surface Plasmon Resonance Sensors for Detection of Chemical and Biological Species

2008

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Section: 28 Proteinsmentioning

confidence: 99%

Surface Plasmon Resonance Sensors for Detection of Chemical and Biological Species

2008

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“…Antibodies are also used in combination with biosensors and other detection technologies. SPR was used as a label-free technology for the simultaneous quantification of α-, β-and κ-casein in raw and heat-treated dairy products (Dupont and Muller-Renaud, 2006). The LODs were of 870, 85 and 470 ng/mL, respectively.…”

Section: Elisamentioning

confidence: 99%

Scientific Opinion on the evaluation of allergenic foods and food ingredients for labelling purposes

Products¹

2014

EFS2

115

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Following a request from the Food Safety Authority of Ireland, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA Panel) was asked to deliver a scientific opinion on the evaluation of allergenic foods and food ingredients for labelling purposes. In view of the request, the NDA Panel decided to update its previous opinions relative to food ingredients or substances with known allergenic potential listed in Annex IIIa of 2003/89/EC, as amended. These include cereals containing gluten, milk and dairy products, eggs, nuts, peanuts, soy, fish, crustaceans, molluscs, celery, lupin, sesame, mustard and sulphites. The opinion relates to immunoglobulin (Ig)E-and non-IgE-mediated food allergy, to coeliac disease and to adverse reactions to sulphites in food, and it does not address non-immune-mediated adverse reactions to food. It includes information on the prevalence of food allergy in unselected populations, proteins identified as food allergens, cross-reactivities, the effects of food processing on the allergenicity of foods and ingredients, methods for the detection of allergens and allergenic foods, doses observed to trigger adverse reactions in sensitive individuals and risk assessment methodologies that have been used to derive individual and population thresholds for selected allergenic foods. The present opinion relates to immunoglobulin (Ig)E-and non-IgE-mediated food allergy, to coeliac disease and to adverse reactions to sulphites in food, and it does not address non-immune-mediated adverse reactions to food. For each food ingredient or substance listed in Annex IIIa, it includes information on the prevalence of food allergy in unselected populations, on proteins identified as food allergens, on cross-reactivities, on the effects of food processing on the allergenicity of foods and ingredients, on methods for the detection of allergens and allergenic foods, and on doses observed to trigger adverse reactions in sensitive individuals.Immune-mediated adverse reactions to foods manifest with clinical signs and symptoms of variable severity and duration, which may affect different organs and systems. Anaphylactic reactions to food are IgE mediated and may occur at any age. Non-IgE-mediated food allergy includes a wide range of diseases, including protein-induced enterocolitis and eosinophilic oesophagitis.A careful family and clinical history are the basis for diagnosis of food allergy. Food diaries, skin prick tests (SPTs), allergen-specific IgE measurements, food elimination diets and food challenges are part of the standard protocol for the diagnosis of food allergy. A positive SPT indicates sensitisation to the tested food, but it is not diagnostic of food allergy. Allergen-specific serum IgE antibodies similarly denote sensitisation to a particular food, but they are not diagnostic without a clinical history or food challenge. The use of atopy patch tests for the diagnosis of food allergy is controversial. Other available tests have no current role in the diagnosis of food allergy. Diag...

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“…However, it has several drawbacks, such as underestimation of results, a high limit of detection (1 mg mL À1 ) and interference of medium turbidity. More recently, an immunosensor allowing the simultaneous quantification of as 1 -, band k-casein in milk has been developed (Dupont and Muller-Renaud, 2006). This assay consists of a 2-step sandwich strategy, with 2 monoclonal antibodies directed against the N-and C-terminal extremities of each of the caseins, respectively.…”

Section: Caseinsmentioning

confidence: 99%