2003
DOI: 10.1021/ja029460d
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Quantification of Protein−Ligand Interactions by Mass Spectrometry, Titration, and H/D Exchange:  PLIMSTEX

Abstract: Protein-ligand binding and the concomitant conformational change in the protein are of crucial importance in biophysics and drug design. We report a novel method to quantify protein-ligand interactions in solution by mass spectrometry, titration, and H/D exchange (PLIMSTEX). The approach can determine the conformational change, binding stoichiometry, and affinity in protein-ligand interactions including those that involve small molecules, metal ions, and peptides. Binding constants obtained by PLIMSTEX for fou… Show more

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Cited by 157 publications
(199 citation statements)
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“…Differences are caused by global or local conformational changes associated with ligand binding, formation of protein-ligand interfaces, and/or formation of more stabilized secondary structure elements throughout the protein. Both kinetics of exchange and affinity of the protein can be determined, the latter by either PLIMSTEX (Protein-Ligand Interactions by Mass Spectrometry, Titration, and H/D Exchange) [2] or SUPREX (Stability of Unpurified Proteins from Rates of H/D Exchange) [3].…”
Section: H Ydrogen-deuterium (H/d) Exchange Mass Spectrometry (Ms)mentioning
confidence: 99%
“…Differences are caused by global or local conformational changes associated with ligand binding, formation of protein-ligand interfaces, and/or formation of more stabilized secondary structure elements throughout the protein. Both kinetics of exchange and affinity of the protein can be determined, the latter by either PLIMSTEX (Protein-Ligand Interactions by Mass Spectrometry, Titration, and H/D Exchange) [2] or SUPREX (Stability of Unpurified Proteins from Rates of H/D Exchange) [3].…”
Section: H Ydrogen-deuterium (H/d) Exchange Mass Spectrometry (Ms)mentioning
confidence: 99%
“…PLIMSTEX is a new method developed in our laboratory for the quantitative analysis of protein-ligand binding [9]. A plot of the mass difference between the deuterated and non-deuterated protein (deuterium uptake) versus the total ligand concentration (or the ratio of total ligand concentration to the total protein concentration) gives the PLIMSTEX curve ( Figure 1).…”
Section: Overview Of the Modeling Proceduresmentioning
confidence: 99%
“…The interaction between intestinal fatty acid binding protein and potassium oleate involves one-to-one protein-ligand binding as showed by others [27,28] and recently by us using PLIMSTEX [9]. Although we previously published one titration curve and the results of other titrations [9], we chose this system to demonstrate the modeling of a H/D exchange titration and the use of the newly implemented statistical analysis of the uncertainties.…”
Section: One-to-one Protein-ligand Bindingmentioning
confidence: 99%
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