Quantification of Prostate Cancer Metabolism Using <scp>3D Multiecho bSSFP</scp> and Hyperpolarized [<scp>1‐<sup>13</sup>C</scp>] Pyruvate: Metabolism Differs Between Tumors of the Same Gleason Grade

Chowdhury, Rafat; Müller, Christoph; Smith, Lorna; Gong, Fiona; Papoutsaki, Marianthi-Vasiliki; Rogers, Harriet; Syer, Tom; Singh, Saurabh; Brembilla, Giorgio; Retter, Adam; Bullock, Max; Caselton, Lucy; Mathew, Manju; Dineen, Eoin; Parry, Thomas; Hennig, Jürgen; Elverfeldt, Dominik von; Schmidt, Andreas B.; Hövener, Jan‐Bernd; Emberton, Mark; Atkinson, David; Bainbridge, Alan; Gadian, David G.; Punwani, Shonit

doi:10.1002/jmri.28467

Cited by 14 publications

(19 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several approaches have been demonstrated to boost P to the order of unity, i.e., several orders of magnitude above the equilibrium level, to improve NMR detection sensitivity. , Such hyperpolarized (HP) states are created transiently on small exogenous molecules used as contrast agents that can be injected in vivo to probe metabolic transformations. − Hyperpolarized 13 C-labeled carboxylates are favored because they can retain an HP state for minutes in vivo. , HP [1- 13 C]pyruvate is the most developed HP contrast agent, − and it is now under evaluation in over 30 clinical trials according to clinicaltrials.gov.…”

Section: Introductionmentioning

confidence: 99%

¹³C Radiofrequency Amplification by Stimulated Emission of Radiation Threshold Sensing of Chemical Reactions

et al. 2023

Self Cite

View full text Add to dashboard Cite

Conventional nuclear magnetic resonance (NMR) enables detection of chemicals and their transformations by exciting nuclear spin ensembles with a radio-frequency pulse followed by detection of the precessing spins at their characteristic frequencies. The detected frequencies report on chemical reactions in real time and the signal amplitudes scale with concentrations of products and reactants. Here, we employ Radiofrequency Amplification by Stimulated Emission of Radiation (RASER), a quantum phenomenon producing coherent emission of 13C signals, to detect chemical transformations. The 13C signals are emitted by the negatively hyperpolarized biomolecules without external radio frequency pulses and without any background signal from other, nonhyperpolarized spins in the ensemble. Here, we studied the hydrolysis of hyperpolarized ethyl-[1-13C]acetate to hyperpolarized [1-13C]acetate, which was analyzed as a model system by conventional NMR and 13C RASER. The chemical transformation of 13C RASER-active species leads to complete and abrupt disappearance of reactant signals and delayed, abrupt reappearance of a frequency-shifted RASER signal without destroying 13C polarization. The experimentally observed “quantum” RASER threshold is supported by simulations.

show abstract

Section: Introductionmentioning

confidence: 99%

¹³C Radiofrequency Amplification by Stimulated Emission of Radiation Threshold Sensing of Chemical Reactions

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…for tumor grading or therapy response assessment. [3][4][5][6][7][8] Such molecular MRI scan takes about one minute and employs no ionizing radiation.…”

Section: F]fluorodeoxyglucose (Fdg) Positronmentioning

confidence: 99%

In Vivo Metabolic Imaging of [1-13C]Pyruvate-d3 Hyperpolarized By Reversible Exchange With Parahydrogen

Maissin

Gross

Mohiuddin

et al. 2023

Preprint

View full text Add to dashboard Cite

Metabolic magnetic resonance imaging (MRI) using hyperpolarized (HP) pyruvate has shown promise as a non-invasive technique for diagnosing, staging, and monitoring response to treatment in cancer and other diseases. The clinically established method for producing HP pyruvate is dynamic nuclear polarization; however, it is rather expensive and slow. Here, we demonstrate fast (6 min), low-cost production of HP [1-13C]pyruvate-d3 in aqueous solution using Signal Amplification By Reversible Exchange (SABRE), and in vivo metabolic MRI. The injected solution was sterile, non-toxic, pH neutral and contained ≈30 mM [1-13C]pyruvate-d3 polarized to ≈11% (residual 250 mM methanol and 20 µM catalyst). It was obtained by rapid solvent evaporation and metal filtering. The procedure was well tolerated by all four mice studied here. This achievement is a significant step of making HP MRI available to a wider community. Fast, low-cost, and high-throughput parahydrogen-hyperpolarization has become a viable alternative for metabolic MRI of living organisms.

show abstract

“…[1] This carries valuable diagnostic information, since abnormal pyruvate metabolism is associated with various pathologies (e.g., tumour cells exhibit accelerated pyruvate-to-lactate conversion; "Warburg effect"). [2] Pyruvate has emerged as the leading HP MRI agent because of fast cellular uptake and its central position in cellular metabolism at the cross-section of glycolysis, the citric acid cycle, alanine transamination, and lactate fermentation. [3] HP pyruvate is under evaluation in over 30 clinical trials as off 2022.…”

mentioning

confidence: 99%

Over 20% 13C Hyperpolarization for Pyruvate Using Deuteration and Rapid SLIC-SABRE in Mictrotesla Fields

Schmidt

Eills

Dagys

et al. 2023

Preprint

View full text Add to dashboard Cite

Carbon-13 hyperpolarized pyruvate is about to become the next-generation contrast agent for molecular magnetic resonance imaging of cancer and other diseases. Here, efficient and rapid pyruvate hyperpolarization is achieved via Signal Amplification by Reversible Exchange (SABRE) with parahydrogen through synergistic use of substrate deuteration, alternating, and static microtesla magnetic fields. Up to 22% and 6% long-lasting 13C polarization (T1=3.7±0.25min and T1=1.7±0.1min) is demonstrated for the C1 and C2 nuclear sites, respectively. The remarkable polarization levels become possible due to favorable relaxation dynamics at the microtesla fields. The ultra-long polarization lifetimes will be conducive to yielding high polarization after purification, quality assurance, and injection of the hyperpolarized molecular imaging probes. These results pave the way to future in vivo translation of carbon-13 hyperpolarized molecular imaging probes prepared by this approach.

show abstract

Quantification of Prostate Cancer Metabolism Using 3D Multiecho bSSFP and Hyperpolarized [1‐¹³C] Pyruvate: Metabolism Differs Between Tumors of the Same Gleason Grade

Cited by 14 publications

References 30 publications

¹³C Radiofrequency Amplification by Stimulated Emission of Radiation Threshold Sensing of Chemical Reactions

¹³C Radiofrequency Amplification by Stimulated Emission of Radiation Threshold Sensing of Chemical Reactions

In Vivo Metabolic Imaging of [1-13C]Pyruvate-d3 Hyperpolarized By Reversible Exchange With Parahydrogen

Over 20% 13C Hyperpolarization for Pyruvate Using Deuteration and Rapid SLIC-SABRE in Mictrotesla Fields

Contact Info

Product

Resources

About

Quantification of Prostate Cancer Metabolism Using 3D Multiecho bSSFP and Hyperpolarized [1‐13C] Pyruvate: Metabolism Differs Between Tumors of the Same Gleason Grade

Cited by 14 publications

References 30 publications

13C Radiofrequency Amplification by Stimulated Emission of Radiation Threshold Sensing of Chemical Reactions

13C Radiofrequency Amplification by Stimulated Emission of Radiation Threshold Sensing of Chemical Reactions

In Vivo Metabolic Imaging of [1-13C]Pyruvate-d3 Hyperpolarized By Reversible Exchange With Parahydrogen

Over 20% 13C Hyperpolarization for Pyruvate Using Deuteration and Rapid SLIC-SABRE in Mictrotesla Fields

Contact Info

Product

Resources

About

Quantification of Prostate Cancer Metabolism Using 3D Multiecho bSSFP and Hyperpolarized [1‐¹³C] Pyruvate: Metabolism Differs Between Tumors of the Same Gleason Grade

¹³C Radiofrequency Amplification by Stimulated Emission of Radiation Threshold Sensing of Chemical Reactions

¹³C Radiofrequency Amplification by Stimulated Emission of Radiation Threshold Sensing of Chemical Reactions