Quantification of preeclampsia-related microRNAs in maternal serum

Abstract

Abstract. To identify the specific serum preeclampsia (PE)-related biomarkers, 10 microRNAs (miRNAs) were selected based on their reported aberrant (4 upregulated and 6 downregulated) expression in PE placenta. A total of 1,035 pregnant patients were enrolled. Finally, 32 pregnancies with PE and 32 healthy pregnancies were incorporated in the study. The expression of these 10 miRNAs in the different trimesters was determined by SYBR-Green reverse transcription-quantitative polymerase chain reaction. Compared with that in the healthy controls, the expression levels of miR-152, miR-183 and miR-210 in PE serum were higher in the second and third trimester, whereas the expression of miR-182 was only higher in the third trimester. The expression levels of 6 miRNAs (miR-1, miR-328, miR-363, miR-377, miR-500 and miR-584) that were downregulated in PE placenta showed no significant differences between pregnancies complicated by PE and healthy pregnancies throughout the 3 trimesters. Areas under the receiver operating characteristic [standard error (SE)] during the 20-24th gestational week for predicting PE were 0.026), 0.031) and miR-210: 0.93 (SE, 0.018). In conclusion, the expression levels of serum miR-152, miR-183 and miR-210 were elevated since the second trimester in pregnancies complicated with PE, indicating their potentials as serum biomarkers for forecasting PE.

Differential microRNA expression in human placentas of term intra-uterine growth restriction that regulates target genes mediating angiogenesis and amino acid transport

“…We observed that most of the up-regulated miRs regulated genes relevant to hypoxia responses [35–39], vascular disease [40–44], metabolism [45–49], inflammation [50–51], or were previously reported in pre-eclamptic placentas [52–55]. In contrast, the down-regulated miRs were for the most part relevant for genes with a role in obesity or cancers [56–58], though several had also been reported in association with pregnancy disorders involving placental insufficiency [52, 55,59–61]. Given our focus on up-regulated miRs with the potential of down-regulating target genes, selected genes in the top 50 miRs which were differentially expressed (up-regulated) between groups are listed to the right of the figure, including miR-10b and -363 , which were further validated in subsequent studies.…”

Estrogen Promotes cAMP Production in Mesenchymal Stem Cells by Regulating ADCY2

“…It has been reported that miR-148a/152 was upregulated in the placenta of preeclamptic rats (31). The expression of miR-152 is increased in the placental tissues and serum of PE, and this increased miR-152 expression can promote the apoptosis of trophoblast cells (32,33). Furthermore, miR-148a could modulate differentiation of MSCs (34), and the expression and function of miR-148a /152 were associated with estrogen signaling pathway (35)(36)(37)(38).…”

MicroRNA-218-5p Promotes Endovascular Trophoblast Differentiation and Spiral Artery Remodeling

“…64 Therefore, our findings regarding miR-218-5p may warrant further investigation into other gestational disorders. The use of miRNAs as potential plasma biomarkers has been investigated, 28,65 and the utility of miR-218-5p as both a predictive and prognostic marker in cancer has been suggested. 66,67 The possibility of using miR-218-5p as a biomarker of PE and possibly other disorders of pregnancy remains to be investigated.…”