Quantification of polyhydroxyalkanoates in mixed and pure cultures biomass by Fourier transform infrared spectroscopy: comparison of different approaches

Isak, Ilena; Patel, Meeta; Riddell, Mark Jc; West, Mark; Bowers, Tracey; Wijeyekoon, Suren; Lloyd, J. A.

doi:10.1111/lam.12605

Cited by 28 publications

(21 citation statements)

References 18 publications

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“…The stretching vibration of the C–O–C group was observed at around 1222–1225 cm −1 . [ 38 ] The peaks at 3434, 2925, 1459, 1384 cm −1 corresponded to the functional groups –OH, CH, CH 2 , CH 3 present in the polymer. These bands indicate the presence of hydroxybutyrate polymer, and the results are in agreement with those reported elsewhere.…”

Section: Resultsmentioning

confidence: 99%

A comparative analysis of perforation and blister features on internally corroded aged water pipeline wall

Martinez

Ilhan-Sungur

Cansever

et al. 2022

Materials & Corrosion

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Despite well-controlled chlorination, no known operational outages and physicochemical parameters of the water indicating a slight tendency to form carbonate deposits, aged and internally uncoated pipelines may experience unexpected internal corrosion events that raise integrity concerns. A typical corrosion damage was investigated consisting of a single perforation covered by a corrosion product cap (CPC) approximately 5 cm in diameter and surrounded by a relatively dense population of blisters of approx. 1 cm in diameter covering shallow corrosion pits. It was observed on the inner wall of an aged on-land water distribution pipeline after decades of corrosion-free operation. A dense population of bacteria, sulphides and extracellular polysaccharide substances were detected in the CPC, but none at the pit bottom surrounding the perforation and within the blisters, but abundant fungi were observed on scanning electron microscopy images of the blister bottom. The morphology of the blisters suggests that the blister formation is due to the loss of adhesion of the corrosion product layer, which is influenced by the formation of spherulitic crystals. The Fourier transform infra-red spectroscopy results suggest that the growth of these carbonate crystals is related to the adsorption of microbially generated carbonyl groups. The results support a likely scenario of the effect of biomineralization on the appearance of corrosion through the formation of blisters, which may provide a niche for microbes that eventually cause corrosion damage.biomineral (CaCO 3 ), extracellular polysaccharide substances (EPS), microbiologically induced corrosion (MIC), water distribution system | INTRODUCTIONCorrosion is one of the main problems encountered in the water utility industry and metallic piping systems. It poses the most common threat to the performance and life span of the pipeline system also causes a substantial threat to human life and safety. Internal corrosion of a metallic water pipeline is a function of the physicochemical and biological parameters of the water in contact with the pipeline walls. In terms of potable water pipelines, dissolved oxygen, pH, temperature, alkalinity, buffer intensity, the impurities, rate of the water flow, water stagnation time and microbial activity have noticeable effects on corrosion of the internal side of steel

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Section: Resultsmentioning

confidence: 99%

A comparative analysis of perforation and blister features on internally corroded aged water pipeline wall

Martinez

Ilhan-Sungur

Cansever

et al. 2022

Materials & Corrosion

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“…The analysis of the functional groups was carried out in the range of values of the wavenumber of 4000-550 cm -1 (Figure 4). A stretching of the band in 1723 cm -1 was found belonging to the carbonyl bond (C=O) caused by the PHA [42]. In turn, other common bands were identified between 1100 -1300 cm -1 showing a stretching of the C-O bond of the ester group [43].…”

Section: Characterization Of Extracted Biopolymermentioning

confidence: 89%

Production of Polyhydroxyalkanoate by Bacillus thuringiensis Isolated from Agricultural Soils of Cascas-Peru

Cueva-Almendras

Alvarado

Fuentes-Olivera

et al. 2022

Braz. arch. biol. technol.

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Polyhydroxyalkanoates (PHA) are biodegradable biopolymers of microbial origin that can be alternative materials to decrease the extensive use of plastics of petrochemical or synthetic origin. Thus, the selection of microorganisms with potential for PHA production from unexplored natural sources is a strategy to find bacterial species of high value. In the current study, 55 microbial strains related to bacteria were isolated from agricultural soils from Cascas, Peru. Initially, 4 strains were selected by Sudan Black B staining and Nile blue A fluorescence methods, subsequently, they were screened to examine its production capacity of PHA. Bacillus thuringiensis SP7-1 strain was selected based on its high production of PHA at 0.54 ± 0.16 g/L with an accumulation of 19 % by weight of cell biomass, during 72 h at 30 ºC. The isolate was characterized by its morphology, biochemical and molecular tests through the 16S rRNA gene. The extracted HIGHLIGHTS• Bacillus thuringiensis SP7-1 from agricultural soils producing polyhydroxyalkanoate.• The strains SP7-1 with a PHA accumulation of 0.54 g/L and 19 % in dry biomass.• PHA was characterized with FTIR, DSC and TGA with remarkable chemical properties.• A thermic degradation in a range of 270-303°C and a Melting Temperature of 166.88°C.

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“…Semi-quantitative analysis of PHA by FT-IR has been demonstrated for mixed cultures of bacteria using a simple model that employs the ratio between the peak areas of the carbonyl-ester and the amide band I when the PHA content is below 44% of the CDW [ 44 ]. Bacterial cells for FT-IR analysis were either scraped off from agar plates using an inoculation loop, re-suspended in 96% ethanol and spun down in Eppendorf tubes at 21,000× g for 1 min, or 500 µL were taken from liquid cultures and spun down similarly followed by re-suspension of the pellet in 96% ethanol and a second spin to subsequently remove the supernatant.…”

Section: Methodsmentioning

confidence: 99%

High natural PHA production from acetate in Cobetia sp. MC34 and Cobetia marina DSM 4741T and in silico analyses of the genus specific PhaC2 polymerase variant

et al. 2021

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Background Several members of the bacterial Halomonadacea family are natural producers of polyhydroxyalkanoates (PHA), which are promising materials for use as biodegradable bioplastics. Type-strain species of Cobetia are designated PHA positive, and recent studies have demonstrated relatively high PHA production for a few strains within this genus. Industrially relevant PHA producers may therefore be present among uncharacterized or less explored members. In this study, we characterized PHA production in two marine Cobetia strains. We further analyzed their genomes to elucidate pha genes and metabolic pathways which may facilitate future optimization of PHA production in these strains. Results Cobetia sp. MC34 and Cobetia marina DSM 4741T were mesophilic, halotolerant, and produced PHA from four pure substrates. Sodium acetate with- and without co-supplementation of sodium valerate resulted in high PHA production titers, with production of up to 2.5 g poly(3-hydroxybutyrate) (PHB)/L and 2.1 g poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/L in Cobetia sp. MC34, while C. marina DSM 4741T produced 2.4 g PHB/L and 3.7 g PHBV/L. Cobetia marina DSM 4741T also showed production of 2.5 g PHB/L from glycerol. The genome of Cobetia sp. MC34 was sequenced and phylogenetic analyses revealed closest relationship to Cobetia amphilecti. PHA biosynthesis genes were located at separate loci similar to the arrangement in other Halomonadacea. Further genome analyses revealed some differences in acetate- and propanoate metabolism genes between the two strains. Interestingly, only a single PHA polymerase gene (phaC2) was found in Cobetia sp. MC34, in contrast to two copies (phaC1 and phaC2) in C. marina DSM 4741T. In silico analyses based on phaC genes show that the PhaC2 variant is conserved in Cobetia and contains an extended C-terminus with a high isoelectric point and putative DNA-binding domains. Conclusions Cobetia sp. MC34 and C. marina DSM 4741T are natural producers of PHB and PHBV from industrially relevant pure substrates including acetate. However, further scale up, optimization of growth conditions, or use of metabolic engineering is required to obtain industrially relevant PHA production titers. The putative role of the Cobetia PhaC2 variant in DNA-binding and the potential implications remains to be addressed by in vitro- or in vivo methods.

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Quantification of polyhydroxyalkanoates in mixed and pure cultures biomass by Fourier transform infrared spectroscopy: comparison of different approaches

Cited by 28 publications

References 18 publications

A comparative analysis of perforation and blister features on internally corroded aged water pipeline wall

A comparative analysis of perforation and blister features on internally corroded aged water pipeline wall

Production of Polyhydroxyalkanoate by Bacillus thuringiensis Isolated from Agricultural Soils of Cascas-Peru

High natural PHA production from acetate in Cobetia sp. MC34 and Cobetia marina DSM 4741T and in silico analyses of the genus specific PhaC2 polymerase variant

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