Quantification of polyamines in human erythrocytes using a new near‐infrared cyanine 1‐(ϵ‐succinimidyl‐hexanoate)‐ 1′‐methyl‐3,3,3′,3′‐tetramethyl‐ indocarbocyanine‐5,5′‐disulfonate potassium with CE‐LIF detection

Abstract

A novel near-infrared (NIR) cyanine 1-(epsilon-succinimidyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate potassium (MeCy5-OSu) has been developed in our laboratory. Simultaneous determination of MeCy5-OSu-derivatized polyamines spermine (Spm), spermidine (Spd), cadaverine (Cad), and putrescine (Put) based on the separation by CE combined with diode LIF detection has been accomplished. The highest derivatization efficiency was achieved in 0.2 mol/L borate buffer (pH 8.8) for 20 min at 25 degrees C. Polyamine derivatives were separated within 14 min in the phosphate running buffer (pH 3) containing 50 mmol/L phosphoric acid, 40 mmol/L SDS, and 35% methanol v/v. Linearity of response was obtained in the range of 10-200 nmol/L. The detection limits (S/N = 3) for Spm, Spd, Cad, and Put were 0.8, 1, 3, and 2 nmol/L, respectively. The proposed method has been successfully applied to the analysis of polyamines in erythrocytes of two healthy persons and one cancer patient. Average recoveries for erythrocyte samples were 93.6-106% and coefficients of variation ranged from 1.8 to 5.4%. The analysis of polyamines in erythrocytes can be used for studying the relationship between their changes and the carcinogenesis process involved in erythrocytes.

Analysis of short‐chain aliphatic amines in food and water samples using a near infrared cyanine 1‐(ε‐succinimydyl‐hexanoate)‐1′‐methyl‐3,3,3′,3′‐tetramethyl‐indocarbocyanine‐5,5′‐disulfonate potassium with CE–LIF detection

“…Such experimental results could be due to the little differences of charge/mass ratio between each amine derivative. In our previous work using MeCy5‐OSu as the derivatizing reagent for polyamines, SDS‐mediated and methanol‐modified MEKC was proved to be effective in improvement of peak shape and resolution . In this work, BGE containing phosphate buffer, SDS, and methanol was also applicable in effective separation of aliphatic amine derivatives.…”

“…Under this condition, thermolysis was absent, signal quenching was minimized, and derivatization yield was sufficient to bring about the high sensitivity of the method. The mild derivatization conditions for aliphatic amines (borate buffer, pH 9.0, 20°C, and 30 min) and polyamines analogs (borate buffer, pH 8.8, 25°C, and 20 min) exhibited the high reactivity of MeCy5‐OSu with aliphatic amine component.…”

“…The running buffer was prepared by dissolving phosphoric acid, SDS, and methanol into deionized water and the solution pH was adjusted by using 1.0 mol L −1 NaOH before diluting to final volume. Storage and usage of MeCy5‐OSu was performed using previously reported methodologies .…”

“…1‐(ε‐Succinimydyl‐hexanoate)‐1′‐methyl‐3,3,3′,3′‐tetra‐methylindocarbocyanine‐5,5′‐disulfonate potassium (MeCy5‐OSu), an amine‐derivatizating reagent proposed by us, possesses many desirable analytical properties, such as near‐infrared excitation/emission wavelength (649/667 nm), water‐soluble, amine‐responsive, and compatible with 635 nm diode laser detector, allowing it to be well applied in quantitative determination of cancer‐related polyamines in human erythrocyte and rapid analysis of phosphoamino acids from phosvitin . In this work, the suitability of MeCy5‐OSu as a derivatizing reagent for short‐chain aliphatic amines was investigated.…”

Current Status of Polyamine and Polyamine Analogs Analysis in Cancer Research

“…Simultaneous determination of MeCy5-OSu-derivatized polyamines spermine, spermidine, cadaverine, and putrescine based on the separation by capillary electrophoresis combined with diode LIF detection has been accomplished by Fu et al (75). The analysis of polyamines in erythrocytes can be used for studying the relationship between their changes and the carcinogenesis process involved in erythrocytes.…”