Quantification of phytochemicals and in vitro antioxidant activities from various parts of Euphorbia neriifolia Linn.

Priya, C. N. Bharathi; Janmeda, Pracheta

doi:10.7324/jabb.2022.100217

“…The IC 50 values of the extracts were 0.00017 μ g/ml and 4.617 μ g/ml for the aqueous and ethanol. A similar study carried out on Euphorbia neriifolia of the same family had an IC 50 of 76.2 ± 0.07 inhibition [ 30 ]. This could be justified by the fact that all plants belonging to the same family do not necessarily have the same mode of action.…”

Section: Discussionmentioning

confidence: 92%

“…This could be justified by the fact that all plants belonging to the same family do not necessarily have the same mode of action. Bridelia micrantha extract has the ability to donate protons that can serve as an inhibitor or scavenger of free radicals, acting as a primary [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Antiplasmodial, Antioxidant, and Cytotoxic Activity of Bridelia micrantha a Cameroonian Medicinal Plant Used for the Treatment of Malaria

Kevin

¹

,

Cédric

²

,

Nadia

³

et al. 2023

BioMed Research International

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Introduction. Resistance to common antimalarial drugs and persistence of the endemicity of malaria constitute a major public health problem in Cameroon. The aim of this study was to evaluate the in vitro antiplasmodial, antioxidant, and cytotoxic activities of aqueous and ethanol extracts of Bridelia micrantha used by Cameroonian traditional healers for the treatment of malaria. Methods. Aqueous and ethanolic stem bark extracts were prepared according to standard procedures. The SYBR Green method was used for antiplasmodial activity on strains of Plasmodium falciparum sensitive to chloroquine (3D7) and resistant (Dd2). In vitro antioxidant activities of B. micrantha were determined using the scavenging activity of 2,2 ′ -diphenyl-1-picrylhydrazyl, nitric oxide, ferric reducing power, and hydrogen peroxide as well as their cytotoxicity on RAW 264.7 macrophage cells and red blood cells (RBC). Results. The aqueous and ethanol extracts of Bridelia micrantha showed antiplasmodial activity on the 3D7 strain with I C 50 of 31.65 ± 0.79 μg/ml and 19.41 ± 2.93 μg/ml, respectively, as well as 37.64 ± 0.77 μg/ml and 36.22 ± 1.04 μg/ml for the Dd2 strain, respectively. The aqueous and ethanol extracts showed free radical scavenging properties. The IC 50 aqueous and ethanol extract was approximately 0.0001737 μg/ml, 42.92 μg/ml, 1197 μg/ml, 63.78 μg/ml and 4.617 μg/ml, 429.9 μg/ml, 511 μg/ml, and 69.32 μg/ml for DPPH, NO, H2O2, and FRAP, respectively, which were compared to ascorbic acid ( 8.610 e − 005 μg/ml, 2901 μg/ml, 3237 μg/ml, and 18.57 μg/ml). The aqueous and ethanol extracts of B. micrantha were found to be nontoxic with CC 50 values of 950 ± 6.6 μg/ml and 308.3 ± 45.4 μg/ml, respectively. Haemolysis test showed that the two extracts were not toxic. Conclusion. These results suggest that B. micrantha can serve as an antimalarial agent. However, further studies are needed to validate the use of B. micrantha as an antimalarial.

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“…Distilled water was added to the solution to make up a final volume of 10 mL. The solution was allowed to stand for 7 min before the absorbance by a spectrophotometer UV-Vis (UV VIS Spectrophotometer Labo-7809) at 700 nm [7]. The concentration of the tannin was expressed as mg/ml GAE.…”

Section: Estimation Of Tannin Contentmentioning

confidence: 99%

Phytochemical Analysis, Anti-Inflammatory, and Antioxidant Activity of Selected Medicinal Plants in Mandiangin Rainforest in South Kalimantan, Indonesia

Nugroho

¹

,

Budianto²,

Siahaan³

et al. 2023

J.Trop.Life.Science

1

0

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Mandiangin is one of the tropical rainforests in South Kalimantan, with highly diverse plants used for health and medicinal purposes. To date, scientific evidence on these plants' content and bioactivities remain lacking for further medical applications. In this perspective, this study aimed to determine the phytochemical contents of six selected plants, which include the Bilaran Kusan (Passiflora foetida), Sembilakan (Caesalpinia sp), Bamban Batu (Donax cenniformis), Kilayu (Aglaia sp), Ulur-Ulur (Tetrastigma sp), and Mali-Mali (Leea indica) from the Mandiangin rainforest, in addition to their antioxidant and anti-inflammatory activity. Results revealed that the Leea indica extracts exhibited the highest flavonoid content (70.892 ± 0.34 mg/ml QE), while the highest tannin (2.101 ± 0.02 mg/ml GAE) and alkaloid contents (25.30 ± 0.71 %) were recorded for the Aglaia sp species. Meanwhile, Passiflora foetida showed the highest saponin content at (31.78 ± 2.97 %). Based on the study findings, the extracts of all six plants from the Mandiangin rainforest possessed medically valuable phytochemical constituents, as seen from their appreciable antioxidant and anti-inflammatory activity. Of all these plants, P. foetida and L. indica demonstrated the highest antioxidant activity, whereas the highest anti-inflammatory activity was noted for the P. foetida, Aglaia- and Caesalpinia species.

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“…The Total Saponin Content (TSC) in plant parts was estimated by modified method as explained by Jain et al [21,22] . Then the total saponin compounds content in plant extracts was calculated as Saponin Quillaja Equivalent (SQE) per 50 g of fresh mass using above equation [23] .…”

Section: Quantitative Phytochemical Assaysmentioning

confidence: 99%

In Vitro Antioxidant Assay of Different Phytoconstituents and Extraction Optimization of Phenolics from Cyperus rotundus L

Varshney¹,

Prakash²,

Singh³

et al. 2023

IJPS

0

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Oxidative stress related disorders occur as a result of free radicals in the cellular organs. Antioxidants provide protection contrary to oxidative stress by counteracting free radicals through the help of different bioactive constituents present in the plants. The aim of this study is to find out the quantitative estimation of phytochemicals (phenolics, tannins, flavonoid, non-tannins, alkaloid and saponins), in vitro antioxidant activity (2, 2-diphenyl-1-picryl-hydrazyl-hydrate, metal chelating, ferric reducing, superoxide dismutase and total antioxidant activity) of different parts of Cyperus rotundus. Along with this, the extraction of polyphenolic compounds by conventional and non-conventional methods and their optimization by response surface methodology technique were also studied. As the outcomes of the study, maximum amount of total phenolics content was found in the ethanolic fraction of stem (37.88±0.94 mg gallic acid equivalent/g). The root has shown the highest flavonoid (0.24±0.03 mg QAE/g) and non-tannin content (21.55±2.43 mg gallic acid equivalent/g) in the extract of petroleum ether. The total alkaloids and tannin content in flower and root i.e., 5.05±0.08 mg caffeine equivalent/g and 76.28±0.62 mg tannic acid equivalent/g was found to be better in aqueous extract respectively. The fraction of ethyl acetate has reported the greatest saponin content (39.2±3.8 mg gallic acid equivalent/g). In case of flower part half-maximal inhibitory concentration value of 2, 2-diphenyl-1-picryl-hydrazyl-hydrate and superoxide dismutase antioxidant activity varies from 0.57-1.90 mg/ml and from 0.053-1.401 mg/ml respectively. Antioxidant activity of each part of the plant was reported in the fraction of ethanol and water by fluorescence recovery after photobleaching (0.403±0.009-0.886±0.02 mg/ml), metal chelation activity (0.010±0.0-0.306±0.001 mg/ml) and total antioxidant capacity (0.030±0.0-0.317±0.002 mg/ml). Microwave assisted extraction method was found to be the suitable one for optimization of total phenolic content. So, the relationship between different parameter was determined through response surface methodology technique and evaluated by plotting the graphs. Polar extracts have found to show the maximum quantity of secondary bioactive metabolites and antioxidant activity as compared to non-polar one. And among the comparison between conventional and non-conventional methods, ultra sonication (nonconventional method) has shown the highest yield of bioactive phenolic compound. So, this study shows that Cyperus rotundus has wide range of secondary metabolites showing antioxidant properties that can be applied for different therapeutic applications.

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Quantification of phytochemicals and in vitro antioxidant activities from various parts of Euphorbia neriifolia Linn.

Cited by 11 publications

References 50 publications

Antiplasmodial, Antioxidant, and Cytotoxic Activity of Bridelia micrantha a Cameroonian Medicinal Plant Used for the Treatment of Malaria

Antiplasmodial, Antioxidant, and Cytotoxic Activity of Bridelia micrantha a Cameroonian Medicinal Plant Used for the Treatment of Malaria

Phytochemical Analysis, Anti-Inflammatory, and Antioxidant Activity of Selected Medicinal Plants in Mandiangin Rainforest in South Kalimantan, Indonesia

In Vitro Antioxidant Assay of Different Phytoconstituents and Extraction Optimization of Phenolics from Cyperus rotundus L

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