Quantification of phenolics in Syzygium cumini seed and their modulatory role on tertiary butyl-hydrogen peroxide-induced oxidative stress in H9c2 cell lines and key enzymes in cardioprotection

Abstract

The study quantified the major phenolics in different fractions of seeds and evaluated their cardioprotective efficacy. Gallic acid, ellagic acid, cinnamic acid, quercetin, syringic acid and ferulic acid were the major polyphenols present in different fractions of seeds. The cardioprotective effect of seed fractions in modulating angiotensin converting enzyme (ACE), HMG-CoA reductase, LDL oxidation and tertiary butyl hydrogen peroxide (TBHP) induced oxidative stress in H9c2 cardiac cell lines were investigated. effectively attenuated the cellular oxidative stress in H9c2 cardiomyoblasts. These fractions possessed inhibitory potential against ACE, HMG-CoA reductase and LDL oxidation. Molecular docking studies of the predominant polyphenols with ACE and HMG-CoA proteins revealed the binding interactions of these compounds, thus confirming their modulation of activity. The present study demonstrated the cardioprotective efficacy of seed fractions which can be attributed to the presence of phenolic acids and flavonoids.

Insights into the phenolic compounds present in jambolan (Syzygium cumini) along with their health‐promoting effects

“…Syama et al . () reported that jambolan seed extract (obtained sequentially using hexane, ethyl acetate, methanol and water as solvents) effectively reduced the oxidative stress in H9c2 cell lines. Moreover, the extract fractions possessed inhibitory action against LDL oxidation and different enzymes (angiotensin‐converting enzyme and HMG‐CoA reductase).…”

“…The antihypertensive effect of jambolan leaf extract might be due to the presence of flavonoids in jambolan leaf extract, as they have role in inhibition of arterial tone by the blockade of extracellular calcium influx (Ribeiro et al, 2014). Syama et al (2017) reported that jambolan seed extract (obtained sequentially using hexane, ethyl acetate, methanol and water as solvents) effectively reduced the oxidative stress in H9c2 cell lines. Moreover, the extract fractions possessed inhibitory action against LDL oxidation and different enzymes (angiotensin-converting enzyme and HMG-CoA reductase).…”

“…Apart from this, jambolan is also found in some areas of the United States such as Florida. Fruits, seeds, leaves and bark of this plant are edible, having a long history of medicinal as well as culinary uses in India (Syama et al, 2017). These plant parts are a versatile source of beneficial phytochemical constituents having bioactive potential (Prince et al, 1998;Ayyanar & Subash-Babu, 2012).…”

Valuable Food Molecules with Potential Benefits for Human Health

“…Kaempferol, rutin, luteolin, quercetin, resveratrol Activity against doxorubicin-induced cardiotoxicity [119] Gallic, ellagic, syringic, ferulic, cinnamic acids, and quercetin Attenuate oxidative stress in H9c2 cardiomyoblasts [120] Aspalathin and phenylpyruvic acid-2-O-β-D-glucoside Protect myocardial infarction caused by chronic hyperglycemia [121] Puerarin…”

Syzygium cumini extract induced reactive oxygen species‐mediated apoptosis in human oral squamous carcinoma cells

“…The seeds of the plant contain various phytoconsitutents such as alkaloid, jambosine, and glycoside jambolin or antimellin . In vivo S. cumini is shown to have antimicrobial, antidiabetic, and antioxidant cardioprotective antiobesity properties. In vitro context, cytotoxic effect of S. cumini has been reported in breast cancer cell line .…”