Quantification of neutral human milk oligosaccharides by graphitic carbon high-performance liquid chromatography with tandem mass spectrometry

Bao, Yuanwu; Chen, Ceng; Newburg, David S.

doi:10.1016/j.ab.2012.10.003

Cited by 99 publications

(97 citation statements)

References 48 publications

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“…A method using graphite carbon HPLC- MS indicated that milk oligosaccharides differ between lactating mothers and also vary during the lactating period. 82 An LC-MS/MS method was used to investigate and quantify glycosaminoglycans in the urine of patients with MPS I, II, and VI. 83 …”

Section: Quantificationmentioning

confidence: 99%

Oligosaccharide Analysis by Mass Spectrometry: A Review of Recent Developments

Kailemia¹,

et al. 2013

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Section: Quantificationmentioning

confidence: 99%

Oligosaccharide Analysis by Mass Spectrometry: A Review of Recent Developments

Kailemia¹,

et al. 2013

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“…Oligosaccharides were quantified by an LC-MS consisting of a capillary HPLC (1100 Series LC, Agilent) controlled by ChemStation software (Agilent) [33]. Chromatographic resolution was on a stationary phase of porous graphitic carbon (100 × 2.1 mm, 3-µm particle size, Hypercarb, Thermo Fisher Scientific) at ambient temperature with a mobile phase of 10 % acetonitrile (Optima, Thermo Fisher) in 90 of 0.02 % (v/v) acetic acid in Milli-Q deionized water with a flow rate of 0.5 mL/min and a 5-min run time.…”

Section: Analysis Of Oligosaccharidesmentioning

confidence: 99%

Relative fermentation of oligosaccharides from human milk and plants by gut microbes

Wang

Chen

et al. 2016

Eur Food Res Technol

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sparse. B. longum fermentation products inhibited C. jejuni and E. coli. Thus, HMOS most strongly promoted growth of the two mutualists, and both HMOS and GMOS were efficiently fermented by these mutualists into organic acids. This is consistent with a primary role of HMOS in guiding early colonization of the infant microbiota by mutualist symbionts, and of plant oligosaccharides, especially GMOS, in maintaining a favorable microbiota through adulthood.Keywords Human milk oligosaccharides · Plant oligosaccharides · Microbiota · Prebiotic · Organic acids Abbreviations HMOSHuman milk oligosaccharides GMOS Gluco-manno-oligosaccha-Abstract Gut microbiota is important to human health. Specific dietary glycans promote favorable microbiota growth and inhibit pathobionts. Dietary glycans most relevant to adults and weaned infants are derived from plants or lactose; human milk oligosaccharides (HMOS) are most relevant to breastfed infants. Their efficacy in supporting bacterial growth is compared to determine their potential roles in the initiation and maintenance of colonization. Bioactivities of gluco-manno-oligosaccharides (GMOS), galacto-oligosaccharides (GOS), xylo-oligosaccharides (XOS), cellobiose (CBS), HMOS, and the most prominent individual HMOS, 2′-fucosyllactose (2′-FL) were contrasted. Two representative gut microbiota mutualists, Bifidobacteria longum ATCC15697 and Lactobacillus acidophilus NRRL B-4495, and two non-mutualists, Campylobacter jejuni S107 and Escherichia coli K12, were used to assess the in vitro prebiotic potential of these oligosaccharides. All oligosaccharides afforded growth of B. longum and L. acidophilus, with HMOS supporting the most robust growth, while none of these oligosaccharides afforded meaningful growth of non-mutualists. B. longum efficiently converted HMOS, GMOS, GOS, and XOS into organic acid fermentation products, and, to a lesser degree, L. acidophilus metabolized HMOS, GMOS, and GOS. Fermentation of these glycans by C. jejuni and E. coli was

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“…Three classes of oligosaccharides can be distinguished in human milk: neutral fucosylated (including 2′fucosyllactose, 2′FL), neutral nonfucosylated (including lacto-N-neotetraose, LNnT), and acidic (including 3′sialyllactose, 3′SL, and 6′sialyllactose, 6′SL) (3–5). In contrast, cow's milk contains relatively low oligosaccharide content with limited structural diversity (6).…”

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confidence: 99%

Effects of Infant Formula With Human Milk Oligosaccharides on Growth and Morbidity

Puccio

Alliet

Cajozzo

et al. 2017

J. pediatr. gastroenterol. nutr.

263

313

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Objectives:The aim of the study was to evaluate the effects of infant formula supplemented with 2 human milk oligosaccharides (HMOs) on infant growth, tolerance, and morbidity.Methods:Healthy infants, 0 to 14 days old, were randomized to an intact-protein, cow's milk–based infant formula (control, n = 87) or the same formula with 1.0 g/L 2′fucosyllactose (2′FL) and 0.5 g/L lacto-N-neotetraose (LNnT) (test, n = 88) from enrollment to 6 months; all infants received standard follow-up formula without HMOs from 6 to 12 months. Primary endpoint was weight gain through 4 months. Secondary endpoints included additional anthropometric measures, gastrointestinal tolerance, behavioral patterns, and morbidity through age 12 months.Results:Weight gain was similar in both groups (mean difference [95% confidence interval] test vs control: −0.30 [−1.94, 1.34] g/day; lower bound of 95% confidence interval was above noninferiority margin [−3 g/day]). Digestive symptoms and behavioral patterns were similar between groups; exceptions included softer stool (P = 0.021) and fewer nighttime wake-ups (P = 0.036) in the test group at 2 months. Infants receiving test (vs control) had significantly fewer parental reports (P = 0.004–0.047) of bronchitis through 4 (2.3% vs 12.6%), 6 (6.8% vs 21.8%), and 12 months (10.2% vs 27.6%); lower respiratory tract infection (adverse event cluster) through 12 months (19.3% vs 34.5%); antipyretics use through 4 months (15.9% vs 29.9%); and antibiotics use through 6 (34.1% vs 49.4%) and 12 months (42.0% vs 60.9%).Conclusions:Infant formula with 2′FL and LNnT is safe, well-tolerated, and supports age-appropriate growth. Secondary outcome findings showing associations between consuming HMO-supplemented formula and lower parent-reported morbidity (particularly bronchitis) and medication use (antipyretics and antibiotics) warrant confirmation in future studies.

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Quantification of neutral human milk oligosaccharides by graphitic carbon high-performance liquid chromatography with tandem mass spectrometry

Cited by 99 publications

References 48 publications

Oligosaccharide Analysis by Mass Spectrometry: A Review of Recent Developments

Oligosaccharide Analysis by Mass Spectrometry: A Review of Recent Developments

Relative fermentation of oligosaccharides from human milk and plants by gut microbes

Effects of Infant Formula With Human Milk Oligosaccharides on Growth and Morbidity

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