Quantification of metallothionein-III in brain tissues using liquid chromatography tandem mass spectrometry

Gunn, Adam P; McLean, Catriona; Crouch, Peter J.; Roberts, Blaine R.

doi:10.1016/j.ab.2021.114326

Cited by 4 publications

(2 citation statements)

References 48 publications

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“…Ke stanovení MT je využíváno široké spektrum metod chemické analýzy 55 . Nejčastěji se využívají elektromigrační metodygelová a kapilární elektroforéza a chromatografické metody 56 . Dále se využívají elektrochemické metody, mezi něž patří diferenční pulsní voltametrie (DPV) nebo derivační chronopotenciometrie s konstantním proudem (CPSA) 49,[57][58][59] .…”

Section: Stanovení Mtunclassified

Study of Metallothionein Levels in the Serum of Patients with Malignant Tumour

Melich,

Fořtová,

Hosnedlová

et al. 2023

Chem. Listy

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Metallothionein (MT) is a low molecular weight intracellular protein, the primary function of which is to maintain the homeostasis of heavy metals in living organisms. Only a few research has been carried on the topic molecular mechanism of MT. Recent research points to its significant relationship to carcinogenesis, spontaneous mutagenesis, and the effectiveness of antitumor drugs. Increased level of MT is studied as a potential indicator of increased metabolic activity in cancer. Electrochemical methods are a suitable analytical tool for MT detection. Temperature-denatured blood serum samples of patients diagnosed with cancer were studied using electrochemical analysis (Brdička's reaction). The obtained voltammograms were evaluated as the area under the curve (AUC response). To compare normal and abnormal MT levels, control serum of healthy probands (n = 38) with an average MT amount of 2.2 µg L–1, median 2.1 µg L–1 was used. In all evaluated blood serum samples of cancer patients (n = 228), the average amount of MT was 22.7 µg L–1, and the median was 18.1 µg L–1. The difference (10-fold) between groups was highly statistically significant. The data obtained from the pilot experiment suggest that the levels of thiol compounds such as MT may have clinical potential in cancer patients.

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Section: Stanovení Mtunclassified

Study of Metallothionein Levels in the Serum of Patients with Malignant Tumour

Melich,

Fořtová,

Hosnedlová

et al. 2023

Chem. Listy

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“…While brain extracts from both Alzheimer's disease (AD) patients and aged-matched controls are neurotrophic, the AD brain extract is approximately two-fold more potent than control extracts at promoting survival of cultured neurons [14,15]. Some have attributed this difference to lower levels of MT-3 in AD brain extracts [17,18], while others have found that MT-3 levels are unchanged in AD [15,19]. A molecular mechanism of growth inhibition by MT-3 has not been elucidated.…”

Section: Introductionmentioning

confidence: 99%

Metallothionein-3 attenuates the effect of Cu²⁺ ions on actin filaments

Lakha

Hachicho

Mehlenbacher

et al. 2022

Preprint

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Metallothionein 3 (MT-3) is a cysteine-rich metal-binding protein that is expressed in the mammalian central nervous system and kidney. Various reports have posited a role for MT-3 in regulating the actin cytoskeleton by promoting the assembly of actin filaments. We generated purified, recombinant mouse MT-3 of known metal compositions, either with zinc (Zn), lead (Pb), or copper/zinc (Cu/Zn) bound. None of these forms of MT-3 accelerated actin filament polymerization in vitro, either with or without the actin binding protein profilin. Furthermore, using a co-sedimentation assay, we did not observe Zn-bound MT-3 in complex with actin filaments. Cu2+ ions on their own induced rapid actin polymerization, an effect that we attribute to filament fragmentation. This effect of Cu2+ is reversed by adding either EGTA or Zn-bound MT-3, indicating that either molecule can chelate Cu2+ from actin. Altogether, our data indicate that recombinant MT-3 does not directly bind actin but it does attenuate the Cu-induced fragmentation of actin filaments.

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Identification and organ-specific patterns of expression of two metallothioneins in the sentinel species Gammarus fossarum

Degli Esposti,

Lalouette,

Gaget

et al. 2024

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Quantification of metallothionein-III in brain tissues using liquid chromatography tandem mass spectrometry

Cited by 4 publications

References 48 publications

Study of Metallothionein Levels in the Serum of Patients with Malignant Tumour

Study of Metallothionein Levels in the Serum of Patients with Malignant Tumour

Metallothionein-3 attenuates the effect of Cu²⁺ ions on actin filaments

Identification and organ-specific patterns of expression of two metallothioneins in the sentinel species Gammarus fossarum

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Quantification of metallothionein-III in brain tissues using liquid chromatography tandem mass spectrometry

Cited by 4 publications

References 48 publications

Study of Metallothionein Levels in the Serum of Patients with Malignant Tumour

Study of Metallothionein Levels in the Serum of Patients with Malignant Tumour

Metallothionein-3 attenuates the effect of Cu2+ ions on actin filaments

Identification and organ-specific patterns of expression of two metallothioneins in the sentinel species Gammarus fossarum

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Metallothionein-3 attenuates the effect of Cu²⁺ ions on actin filaments