Quantification of metabolites in breast cancer patients with different clinical prognosis using HR MAS MR spectroscopy

Abstract

Absolute quantitative measures of breast cancer tissue metabolites can increase our understanding of biological processes. Electronic REference To access In vivo Concentrations (ERETIC) was applied to high resolution magic angle spinning MR spectroscopy (HR MAS MRS) to quantify metabolites in intact breast cancer samples. The ERETIC signal was calibrated using solutions of creatine and TSP. The largest relative errors of the ERETIC method were 8.4%, compared to 4.4% for the HR MAS MRS method using TSP as a standard. The same MR experimental procedure was applied to intact tissue samples from breast cancer patients with clinically defined good (n ¼ 13) and poor (n ¼ 16) prognosis. All samples were examined by histopathology for relative content of different tissue types and proliferation index (MIB-1) after MR analysis. The resulting spectra were analyzed by quantification of tissue metabolites (b-glucose, lactate, glycine, myo-inositol, taurine, glycerophosphocholine, phosphocholine, choline and creatine), by peak area ratios and by principal component analysis. We found a trend toward lower concentrations of glycine in patients with good prognosis (1.1 mmol/g) compared to patients with poor prognosis (1.9 mmol/g, p ¼ 0.067). Tissue metabolite concentrations (except for b-glucose) were also found to correlate to the fraction of tumor, connective, fat or glandular tissue by Pearson correlation analysis. Tissue concentrations of b-glucose correlated to proliferation index (MIB-1) with a negative correlation factor (S0.45, p ¼ 0.015), consistent with increased energy demand in proliferating tumor cells. By analyzing several metabolites simultaneously, either in ratios or by metabolic profiles analyzed by PCA, we found that tissue metabolites correlate to patients' prognoses and health status five years after surgery. This study shows that the diagnostic and prognostic potential in MR metabolite analysis of breast cancer tissue is greater when combining multiple metabolites (MR Metabolomics).

Metabolomics Biomarkers for Breast Cancer

“…This seems to be unregulated in the apparently more aggressive basal-like tumors in this xenograft model. There are also reports that associate high Gly levels with a poor prognosis [28]. …”

In vivo MRS of locally advanced breast cancer: characteristics related to negative or positive choline detection and early monitoring of treatment response

“…The biopsies were placed in cryogenic vials and immediately immersed in liquid nitrogen until further analysis. The biopsies were prepared for ex vivo HR MAS MRS and analyzed as previously described [26]. In brief, tissue (mean wet weight 12.9 mg) was placed in disposable inserts with D 2 O-based phosphate buffered saline (3 µL).…”

Metabolic profile of chronic liver disease by NMR spectroscopy of human biopsies

“…High resolution magic angle spinning (HR-MAS) NMR spectroscopy is a powerful technique for the investigation of metabolites within different intact tissues (25)(26)(27)(28)(29)(30)(31). The precise determination of the biochemical and metabolic profiles in intact tissue promises to extend the possibilities of NMR as a medical diagnostic tool.…”