Quantification of melanocytes in sun-damaged skin

Madden, Kenneth P.; Forman, Seth; Elston, Dirk M.

doi:10.1016/j.jaad.2010.05.021

Cited by 17 publications

(12 citation statements)

References 20 publications

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“…Even though statistical significance was achieved for some criteria, it is important to note that some of the sun‐damaged scouting biopsies displayed features of MIS: (a) nests, (b) epithelioid cytology, and (c) clustering of melanocytes (Figure ). Although these findings contradicted some previous studies, they supported many others, emphasizing that these findings alone should not support an unequivocal diagnosis of melanoma in situ. Although the occurrence of these features in the scouting biopsies was rare, these findings underscore the fact that histologic features of photodamaged skin can overlap with the histopathological features of MIS.…”

Section: Discussioncontrasting

confidence: 87%

Is melanocyte density our last hope? Comparison of histologic features of photodamaged skin and melanoma in situ after staged surgical excision with concurrent scouting biopsies

et al. 2019

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Differentiating melanocytic hyperplasia (MH) on photodamaged skin from junctional lentiginous melanocytic proliferations (JLMP), early evolving melanoma in situ (MIS), or the periphery of a lesion of MIS on staged excision can be challenging. Although previous cross-sectional studies have elucidated important criteria for distinguishing MH on photodamaged skin from more concerning lesions, this study highlights a technique to treat JLMP and MIS with staged mapped excision and baseline scouting biopsies of adjacent nonlesional photodamaged skin to assist in determination of surgical margin clearance. Additionally, we compare the lesional and photodamaged control biopsies from the same patient to evaluate relevant histologic criteria that may be used to distinguish MH in photodamaged skin from JLMP/MIS, while minimizing confounding factors. There was a statistically significant difference (P ≤ 0.05) found for melanocyte density, irregular melanocyte distribution, melanocyte clustering, follicular infundibulum involvement, and nesting. However, criteria such as nesting, epithelioid cells and melanocyte clustering were seen in both photodamaged skin and MIS. These findings underscore the fact that histologic features of photodamaged skin can overlap with the histopathological features of MIS. Of all of the criteria evaluated, melanocytic density was the most objective histologic criterion and did not show overlap between the sun-damaged and JLMP/MIS groups. K E Y W O R D S atypical junctional melanocytic hyperplasia, lentigo maligna, melanoma in situ, scouting biopsy, slow Mohs, staged excision How to cite this article: Speiser J, Tao J, Champlain A, et al. Is melanocyte density our last hope? Comparison of histologic features of photodamaged skin and melanoma in situ after staged surgical excision with concurrent scouting biopsies.

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Section: Discussioncontrasting

confidence: 87%

Is melanocyte density our last hope? Comparison of histologic features of photodamaged skin and melanoma in situ after staged surgical excision with concurrent scouting biopsies

et al. 2019

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“…In embryos, as much as 2301±41 melanocytes per square mm have been observed [5]. In chronically sun-exposed skin, melanocytes in a ratio of one melanocyte for every tree keratinocyte have been observed [27]. The mechanism responsible for the melanocyte distribution must be able to function within this observed range.…”

Section: Resultsmentioning

confidence: 99%

Understanding the Melanocyte Distribution in Human Epidermis: An Agent-Based Computational Model Approach

et al. 2012

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The strikingly even color of human skin is maintained by the uniform distribution of melanocytes among keratinocytes in the basal layer of the human epidermis. In this work, we investigated three possible hypotheses on the mechanism by which the melanocytes and keratinocytes organize themselves to generate this pattern. We let the melanocyte migration be aided by (1) negative chemotaxis due to a substance produced by the melanocytes themselves, or (2) positive chemotaxis due to a substance produced by keratinocytes lacking direct physical contact with a melanocyte, or (3) positive chemotaxis due to a substance produced by keratinocytes in a distance-to-melanocytes dependent manner. The three hypotheses were implemented in an agent-based computational model of cellular interactions in the basal layer of the human epidermis. We found that they generate mutually exclusive predictions that can be tested by existing experimental protocols. This model forms a basis for further understanding of the communication between melanocytes and other skin cells in skin homeostasis.

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“…This skin frequently contains numerous other pigmented lesions such as lentigines, pigmented actinic keratoses, seborrheic keratoses, ephelides and melanocytic nevi. These lesions, in addition to the presence of melanocytic hyperplasia in the background of chronic sun damage [65], serve only to complicate defining the clinical and the histological margins of the tumor.…”

Section: Local Recurrence Riskmentioning

confidence: 99%

Mohs surgery for melanoma: rationale, advances and possibilities

Etzkorn

Cherpelis

Glass

2011

Expert Review of Anticancer Therapy

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Mohs surgery (MS) is an effective technique for the removal of a variety of cutaneous neoplasms by virtue of its thorough assessment of margins. It has yet to become widely accepted for melanoma because recognizing melanocytes histologically in frozen section can be problematic. Recently, 'rapid' methods of immunohistochemistry have been developed that resolve this issue by staining the melanocytes in frozen section. In addition, some of the immunohistochemistry protocols that previously required up to 1 h now take 19 min or less. These technological enhancements for MS have removed some of the obstacles towards the acceptance of MS as a legitimate option for removal of melanomas, especially poorly demarcated lesions and lesions from the head and neck, the distal extremities and the genitalia. Experience thus far with MS for melanoma has shown lower recurrence rates and improved disease-specific survival compared with historical controls for standard excision, while at the same time minimizing the sacrifice of normal tissue.

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Quantification of melanocytes in sun-damaged skin

Cited by 17 publications

References 20 publications

Is melanocyte density our last hope? Comparison of histologic features of photodamaged skin and melanoma in situ after staged surgical excision with concurrent scouting biopsies

Is melanocyte density our last hope? Comparison of histologic features of photodamaged skin and melanoma in situ after staged surgical excision with concurrent scouting biopsies

Understanding the Melanocyte Distribution in Human Epidermis: An Agent-Based Computational Model Approach

Mohs surgery for melanoma: rationale, advances and possibilities

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