Quantification of lysophosphatidic acids in rat brain tissue by liquid chromatography–electrospray tandem mass spectrometry

Determination of endocannabinoids in nematodes and human brain tissue by liquid chromatography electrospray ionization tandem mass spectrometry

“…In our extraction method, LPAs mostly remain in the water phase. We also found that LPAs significantly coat the small particle stationary phase of the narrow bore reversed phase column, and therefore significantly reduce column efficiency [79]. After brief homogenization of the tissue sample with sonification in ice-cold methanol, we found that a one-step extraction protocol (without the addition of any acid or base) was the most suitable isolation method for AEA and 2-AG [57].…”

New Members of the Mammalian Glycerophosphodiester Phosphodiesterase Family

“…Quantitative Analyses of LPA, Lyso-PC, and Choline Metabolites-The extraction method of Bligh and Dyer (20) was modified according to the procedure by Aaltonen et al (21). The UPLC-MS analysis was carried out using an Acquity UPLC system (Waters, Milford, MA) coupled to an Acquity TQD tandem quadrupole mass spectrometer (Waters) with electrospray ionization in the negative modes.…”

“…The UPLC-MS analysis was carried out using an Acquity UPLC system (Waters, Milford, MA) coupled to an Acquity TQD tandem quadrupole mass spectrometer (Waters) with electrospray ionization in the negative modes. Sample solution was injected onto a BEH C18 column (Waters; 2.1 ϫ 50 mm, 1.7 m) at a flow rate of 0.4 ml/min using gradient elution according to the procedure (21). Analyte detection was performed using multiple-reaction monitoring with the following transition: m/z 409 3 153 for 16:0 LPA.…”

LPA receptor signaling: pharmacology, physiology, and pathophysiology

“…Cmpd, compound; NPSPA, N-palmitoyl serine phosphoric acid; NPTyrPA, Npalmitoyl tyrosine phosphoric acid; NAEPA, N-acyl ethanolamide phosphate; AO-LPA 12b, sn-2-aminooxy analog 12b; a-FMP, ␣-fl uoromethylene phosphonate; a-HMP, ␣-hydroxymethylene phosphonate; TPA, thiophosphatidic acid; DDP, dodecyl phosphate; a-MP, ␣-methylene phosphonate; ODP, octadecenyl phosphate; TP 18:1, oleoyl-thiophosphate; BrP-LPA 19b, ␣-bromomethylene phosphonate analog 19b; TDP, tetradecyl phosphonate; FDP, farnesyl diphosphate. by guest, on www.jlr.org Downloaded from indirect enzymatic assays (34), TLC-GC, LC-MS, and LC-MS/MS (46)(47)(48). These techniques have a growing number of predictive, diagnostic, and therapeutic uses (29,43,49,50).…”