Quantification of lipoic acid from skin samples by HPLC using ultraviolet, electrochemical and evaporative light scattering detectors

Campos, Patrícia Mazureki; Praça, Fabíola Silva Garcia; Bentley, Maria Vitória Lopes Badra

doi:10.1016/j.jchromb.2015.07.029

Cited by 26 publications

(16 citation statements)

References 31 publications

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“…CA recoveries were >85% for all drug concentrations and skin matrices evaluated. Our recovery results are above those of most of the scientific literature reports for experiments on drug extraction from the skin (Campos, Praça, & Bentley, ; Vávrová, Lorencová, Klimentová, Novotný, & Hrabálek, ).…”

Section: Resultssupporting

confidence: 55%

Versatile chromatographic method for catechin determination in development of topical formulations containing natural extracts

Ferreira‐Nunes

Ângelo

Silva

et al. 2017

Biomedical Chromatography

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Catechin is found in several natural sources, as Eugenia dysenterica and Syzygium cumini extracts. Its antioxidant and UV-protective properties suggest a potential use in cosmetic and dermatological formulations. A simple analytical method capable of giving support to experiments performed along the development of topical formulations containing this natural substance (i.e. drug assay, skin permeation and stability studies), however, is still needed. Thus, this work aimed to develop and validate a selective HPLC method for catechin determination during the development of topical formulations. Separation was achieved using an RP-C column (300 × 3.9 mm; 10 μm), with a mobile phase of methanol-phosphoric acid 0.01 m (15: 85, v/v), a flow rate of 0.8 mL/min, temperature set at 40°C and UV detection at 230 nm. The method was linear in a range from 0.5 to 10.0 μg/mL (r = 0.9998), precise with an overall variation coefficient of 5.5% and accurate with catechin recovery from the skin layers >85%. Additionally, the method was sensitive (limit of detection, 0.109 μg/mL; limit of quantification, 0.342 μg/mL) and selective against plant extracts, skin matrices and formulation interferents, as well as catechin degradation products. It was also robust regarding both methodology parameters and analytical stability.

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Section: Resultssupporting

confidence: 55%

Versatile chromatographic method for catechin determination in development of topical formulations containing natural extracts

Ferreira‐Nunes

Ângelo

Silva

et al. 2017

Biomedical Chromatography

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“…All recovery values obtained from the different skin fractions were >85%. In some experiments, the recovery was around 100%, which eliminates the need to use a correction factor following permeation experiments (Campos, Praça, & Bentley, ; De Paula, Martins, & Bentley, ; Vávrová, Lorencová, Klimentová, Novotný, & Hrabálek, ).…”

Section: Resultsmentioning

confidence: 99%

Chromatographic method for clobetasol propionate determination in hair follicles and in different skin layers

Ângelo

Cunha-Filho

Gelfuso

2016

Biomedical Chromatography

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Clobetasol propionate (CLO) is a potent steroid used for the treatment of several dermatological diseases. Recent studies suggest its additional use in alopecia topical treatment, generating a demand for novel formulations with specific delivery into hair follicles. Hence, a selective analytical method for drug quantification in follicular structures and skin layers is required. For this, a simple HPLC-UV method was developed. Quantification was performed using a RP-C column (4.6 mm × 15 cm, 5 μm), with a mixture of methanol-acetonitrile-water (50:15:35 v/v) as mobile phase, a flow rate of 1.2 mL/min, oven temperature of 30°C, injection volume of 50 μL and detection at 240 nm. The optimized conditions enabled a 12 min running with CLO elution at 10.1 min and resolution of 2.424 from skin matrix interferences. Validation was performed in accordance with International Conference on Harmonization guidelines and fulfilled the criteria of selectivity, linearity (0.5-15.0 μg/mL), robustness, precision, accuracy and limits of detection and quantification (0.02 and 0.07 μg/mL, respectively). The validated method was successfully applied for CLO quantification following in vitro skin permeation experiments and differential tape-stripping for hair follicle deposition determination, demonstrating its suitability.

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“…Thus, herein the use of human skin was avoided and the purpose of this study was to provide a comparison of various animal skin types that could serve as skin membrane models for in-vitro penetration studies. The use of porcine ear skin, hairless mouse skin and shed snake skin has been widely studied as animal model membranes to replace human skin (Vecchia and Bunge, 2005;Itoh et al, 1990a;Itoh et al, 1990b;Rigg and Barry, 1990) as well as to evaluate skin permeation enhancers (Campos et al, 2016;Petrilli et al, 2013;Praça et al, 2012;Baby et al, 2008;Lopes et al, 2007;Nunes et al, 2005;Nicolazzo et al, 2003;Hirvonen et al, 1991), once they are easily available, easy to employ and can provide results rapidly (Jung and Maibach, 2015). Porcine ear skin and hairless mouse skin are widely employed, while shed snake skin has also potential to be used as a membrane model based on stratum corneum (SC) penetration rate (Praça et al, 2012;Baby et al, 2008;Nunes et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Different protocols are available to quantify the drug retained into porcine or hairless mouse skins, such as (i) evaluation of drug concentration in SC by the tape stripping technique (Lademann et al, 2009), (ii) evaluation of drug concentration in deeper skin layers by homogenization of skin tissue after tape stripping (viable epidermis without SC and dermis) (Campos et al, 2016;Depieri et al, 2015;Ascenso et al, 2014;Praça et al, 2011), (iii) evaluation of drug concentration in the whole skin tissue by homogenization (Ascenso et al, 2013), and (iv) assessment of skin drug penetration by specific micrometric sectioning of horizontal skin using a cryostat (Praça et al, 2012;Labouta et al, 2011;Echevarria et al, 2003). Among these methods, the homogenization technique of the whole skin tissue is easier, faster and less laborious, being also quite useful to detect a low drug penetration level.…”

Section: Introductionmentioning

confidence: 99%

“…Meanwhile, the quantification of the penetrated drug in each strip or in the aggregate strips depends on sensitivity of the analytical method. Therefore, most studies quantify the penetrated drug into SC using the aggregate of all tape strips (Campos et al, 2016;Estracanholli et al, 2014;Praça et al, 2011;Rossetti et al, 2010;Herkenne et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin models

Praça

Medina

Eloy

et al. 2018

European Journal of Pharmaceutical Sciences

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In vitro skin permeation/penetration studies may be affected by many sources of variation. Herein, we aimed to investigate the major critical procedures of in vitro skin delivery studies. These experiments were performed with model drugs according to official guidelines. The influence of skin source on penetration studies was studied as well as the use of a cryopreservation agent on skin freezing evaluated by transepidermal water loss, electrical resistance, permeation/penetration profiles and histological changes of the skin. The best condition for tape stripping procedure was validated through the evaluation of the distribution of corneocytes, mass of stratum corneum (SC) removed and amount of protein removed using finger pressure, a 2kg weight and a roller. The interchangeability of the tape stripping procedures followed by the epidermis and dermis homogenate and the micrometric horizontal cryostat skin sectioning methods were also investigated, besides the effect of different formulations. Noteworthy, different skin sources were able to ensure reliable interchangeability for in vitro permeation studies. Furthermore, an increased penetration was obtained for stored frozen skin compared to fresh skin, even with the addition of a cryoprotectant agent. The best method for tape stripping was the finger pressure followed by the addition of a propylene glycol solvent leading to better SC removal. Finally, no significant difference was found in skin penetration studies performed by different methods suggesting their possible interchangeability.

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Quantification of lipoic acid from skin samples by HPLC using ultraviolet, electrochemical and evaporative light scattering detectors

Cited by 26 publications

References 31 publications

Versatile chromatographic method for catechin determination in development of topical formulations containing natural extracts

Versatile chromatographic method for catechin determination in development of topical formulations containing natural extracts

Chromatographic method for clobetasol propionate determination in hair follicles and in different skin layers

Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin models

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