Quantification of Lactobacillus gasseri, Enterococcus faecium and Bifidobacterium infantis in a probiotic OTC drug by real-time PCR

Matijašić, Bojana Bogovič; Obermajer, Tanja; Rogelj, Irena

doi:10.1016/j.foodcont.2009.07.001

Cited by 27 publications

(12 citation statements)

References 27 publications

(37 reference statements)

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“…In general, the quantification method in which quantities are expressed in numbers of CFU/ml using standard curves is the most widely used (8,14,15,29). However, many of these studies do not use internal controls (normalizers) and/or do not demonstrate the efficiency of the qPCR system, which can lead to over-or underestimation of the data.…”

Section: Discussionmentioning

confidence: 99%

Potential Fate of Ingested Lactobacillus plantarum and Its Occurrence in Human Feces

Costa

Marcelino-Guimarães

Vilas-Bôas

et al. 2014

Appl Environ Microbiol

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e Lactobacillus plantarum has been used in human clinical trials to promote beneficial effects in the immune system, to alleviate intestinal disorders, and to reduce the risk of cardiovascular disease. It is also involved in many fermentation processes in the food industry. However, information on the fate of ingested L. plantarum is limited. In this study, 61 subjects received daily doses of fermented milk containing 2 ؋ 10 11 cells of L. plantarum Lp115 for different periods of time. The target microorganism was monitored in the fecal microbiota via quantitative PCR (qPCR). L. plantarum was detected and quantified in all of the subjects during the ingestion periods. The differences between the L. plantarum levels at time zero and during all the different ingestion periods were statistically significant (P ‫؍‬ 0.001). However, at 15 and 45 days after discontinuing supplementation, the number of lactobacilli was reduced to the baseline level (those at time zero). A longer period with L. plantarum in the diet did not result in increased levels of this bacterium in the stool, based on postconsumption evaluations (P ‫؍‬ 0.001). The qPCR method was specific and sensitive for L. plantarum quantification in such a complex microbial environment as the gastrointestinal tract.

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Section: Discussionmentioning

confidence: 99%

Potential Fate of Ingested Lactobacillus plantarum and Its Occurrence in Human Feces

Costa

Marcelino-Guimarães

Vilas-Bôas

et al. 2014

Appl Environ Microbiol

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“…; Matijasic et al . ). It seemed that the accuracy of this estimate depended on the specificity of the culture media for the tested strain as well as on the microbial diversity of the products.…”

Section: Resultsmentioning

confidence: 97%

Combination of competitive PCR and cultivation methods for differential enumeration of viable Lactobacillus acidophilus in bio‐yoghurts

Shakeri¹,

Shahidi²,

Mortazavi³

et al. 2018

Int J of Dairy Tech

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To ensure the presence of viable Lactobacillus acidophilus in bio-yoghurts, competitive PCR (cPCR) method was optimised using certain amounts of the recombinant plasmid as a non-homologous competitor. Results showed that this method was well-suited to detect and identify approximately 1.3 9 10 4 cfu/mL of L. acidophilus bacteria. The developed cPCR method was comparable to the cultivation method with a limit of quantification of approximately 10 4 cfu/g L. acidophilus bacteria. However, the use of MRS-bile agar medium alone was not enough for an accurate enumeration of viable L. acidophilus bacteria in commercial bio-yoghurts. We concluded that the combined assay can be used as a more reliable and cost-effective alternative for the quality assurance of acidophilus yoghurts.

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“…The methodology used in the present study is also applicable when identifying other groups of probiotic bacteria. Culture-independent molecular methods for quantifying probiotic bacteria have been developed extensively; however, they have not been widely applied in routine laboratories yet (Matijasic et al 2010). The culture-independent analysis of probiotic products by DGGE, which can be performed in less than 30 h, is a very laborious procedure (Temmerman et al 2003).…”

Section: Resultsmentioning

confidence: 99%

“…As stated above, there are still no official or generally accepted ISO standards for analysing the composition of bacterial species in probiotic products in Europe. In order to obtain functional probiotic products for human consumption, rapid and reliable quality control of such products is crucial (Matijasic et al 2010). The health benefits provided by bifidobacteria to the host, as supported through clinical trials, have resulted in their being widely applied as probiotic components in health promoting products (Cronin et al 2011).…”

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confidence: 99%

Direct identification of bifidobacteria from probiotic supplements

Bunešová¹,

Vlková²,

Rada³

et al. 2014

Czech J. Food Sci.

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Bunešová V., Vlková E., Rada V., Hovorková P., Musilová Š., Kmeť V. (2014): Direct identification of bifidobacteria from probiotic supplements. Czech J. Food Sci., 32: 132-136.The DNA of 14 probiotic supplements was isolated directly from various products without previous cultivation. The bifidobacteria composition declared by the manufacturer was determined by species-specific PCR. Such species found were in accordance with those listed on the products. This approach can be performed in less than 5 h and is applicable to other genera of probiotic bacteria. Bifidobacteria were also detected by culture-dependent analysis and MALDI-TOF MS. These methods drew the same results; however, they are more expensive and time consuming. So, we propose direct identification of bifidobacteria for routine quality control inspections of species composition in probiotic supplements.

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Quantification of Lactobacillus gasseri, Enterococcus faecium and Bifidobacterium infantis in a probiotic OTC drug by real-time PCR

Cited by 27 publications

References 27 publications

Potential Fate of Ingested Lactobacillus plantarum and Its Occurrence in Human Feces

Potential Fate of Ingested Lactobacillus plantarum and Its Occurrence in Human Feces

Combination of competitive PCR and cultivation methods for differential enumeration of viable Lactobacillus acidophilus in bio‐yoghurts

Direct identification of bifidobacteria from probiotic supplements

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