Abstract:Matrix-assisted laser desorption/ionization mass spectrometry
imaging
(MALDI-MSI) has been used to visualize molecular distributions in
various biological samples. While it has succeeded in localizing molecules
ranging from metabolites to peptides, quantitative MSI (qMSI) has
remained challenging, especially in small biological samples like
spheroids. Spheroids are a three-dimensional cellular model system
that replicate the chemical microenvironments of tumors. This cellular
model has played an important role… Show more
“…In a more recent publication, we describe absolute quantification of IR in a time-dependent penetration study using a spheroid model system and MALDI-qMSI. 136 To create the calibration curves, control spheroids were homogeneously sprayed with different concentrations of IR solutions to establish the limit-of-detection (LOD) of IR on the sample as shown in Fig. 2 .…”
Mass spectrometry imaging (MSI) has become increasingly utilized in the analysis of biological molecules. MSI grants the ability to spatially map thousands of molecules within one experimental run in a...
“…In a more recent publication, we describe absolute quantification of IR in a time-dependent penetration study using a spheroid model system and MALDI-qMSI. 136 To create the calibration curves, control spheroids were homogeneously sprayed with different concentrations of IR solutions to establish the limit-of-detection (LOD) of IR on the sample as shown in Fig. 2 .…”
Mass spectrometry imaging (MSI) has become increasingly utilized in the analysis of biological molecules. MSI grants the ability to spatially map thousands of molecules within one experimental run in a...
“…Technologies that enable detection of drugs and their metabolites inside individual cells improve drug delivery and optimization, enhancing therapeutic outcomes, and minimizing adverse effects. 1,2 Typically, instrument-based analytical methods 3–6 are used to measure drugs and their metabolites, but require cell lysis 7,8 or embedding and freezing tissues. This precludes real-time cellular measurements 9 and non-invasive animal studies, 10 thereby limiting studies to bulk cellular populations.…”
We report a genetically encoded aptamer biosensor platform for non-invasive measurement of drug distribution in cells and animals. We combined the high specificity of aptamer molecular recognition with the easy-to-detect...
Pre‐clinical models are essential research tools before novel therapeutic or diagnostic methods can be applied to humans. These range from in vitro cell monocultures to vastly more complex animal models, but clinical translation to humans often fails to deliver significant results. Three‐dimensional organoid systems are being increasingly studied to establish physiologically relevant in vitro platforms in a trade‐off between the complexity of the research question and the complexity of practical experimental setups. The sensitivity and precision of analytical tools are yet another limiting factor in what can be investigated, and mass spectrometry is one of the most powerful analytical techniques available to the scientific community. Its innovative use to spatially resolve biological samples has opened many research avenues in the field of mass spectrometry imaging (MSI). Here, we aim to explore the current scientific landscape in the application of MSI on organoids, with an emphasis on their combined potential to facilitate and improve pre‐clinical studies.This article is protected by copyright. All rights reserved
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.