Quantification of Staphylococcus aureus Adhesion to Equine Bone Surfaces Passivated with Plasmalyte™ and Hyperimmune Plasma

Abstract

Understanding the factors that affect bacterial adhesion to bone will facilitate development of improved intraoperative lavage solutions to reduce the morbidity and mortality associated with postoperative infection.

In vitro bactericidal activity of equine platelet concentrates, platelet poor plasma, and plasma against methicillin-resistant Staphylococcus aureus

“…However, this is the first study that describes the antibacterial effect of equine platelet concentrates (activated or not with CG) and activated PPP against MRSA. Our study corroborates a bacteriostatic effect for the equine plasma against Staphylococcus aureus (Bauer et al 2004). The antibacterial behaviour of the equine blood components evaluated in this study against MRSA should be evaluated taking into account at least 4 factors: 1) concentration of platelets, 2) plasma humoral proteins (Miajlovic et al 2007), 3) presence of platelet activating substance and, 4) time.…”

“…Activated PPP presented a better bactericidal action against MRSA in comparison with the plasma group during the 8, 12 and 24 h, suggesting that the antibacterial effect (against MRSA) of the equine blood components evaluated in this in vitro system may be related with complex cellular and molecular mechanisms that likely include the PLTs (and their degranulation microbicidal proteins) and the humoral components of the plasma (Bauer et al 2004, Moojen et al 2008.…”

“…It is possible that in this in vitro system the equine cellular blood components required of humoral mechanisms (complement system (Miajlovic et al 2007) and G immunoglobulin activation) (Bauer et al 2004) and platelets (Moojen et al 2008) to exhibit a sustained antibacterial activity against MRSA. Possibly, the combination of these two immunologic mechanisms conferred to ePCs and PPP a synergistic bactericidal effect against MRSA and maybe, the absence of PLT in the plasma only produced a slight bacteriostatic effect to this substance.…”

Inhibition of bacterial adhesion on biocompatible zwitterionic SBA-15 mesoporous materials

“…Preliminary bacterial adhesion assays on the different synthesized materials using Gram negative E. coli (ATTCC 25922) as a model bacterium were performed under static conditions, as commonly reported in the literature [25][26][27]. Disk-shaped pieces of 6 mm diameter and 1 mm height were prepared by compacting fractions of 30 mg of dried powders of each synthesized material using 2.75 MPa uniaxial and 3 MPa isostatic pressures.…”

Lysine-Grafted MCM-41 Silica as an Antibacterial Biomaterial

“…Bacterial adhesion assays on MCM-41 and MCM-LYS were tested using gram negative E. coli (assay A) and gram positive S. aureus (assay B) by using an established methodology [ 29 , 30 , 31 , 32 , 33 ]. In brief, disk-shaped pieces of 6 mm diameter and 1 mm height were prepared by compacting fractions of 30 mg of dried powders using 2.75 and 3 MPa uniaxial and isostatic pressure, respectively.…”