Quantification of Hydroxyl Radical Generated from the Fe2+-H2O2 and Cu2+-H2O2 Reaction Systems by Electron Spin Resonance Stop and Flow Technique

Kashima-Tanaka, Midori; Tsujimoto, Yasuhisa; Yamazaki, Masahito

doi:10.5466/ijoms.1.67

Cited by 4 publications

(1 citation statement)

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“…In contrast, Fe 2+ was able to react with H 2 O 2 and generate •OH based on the Fenton reaction. However, 200 nM of Fe 2+ caused a slight increase in the trapping length, i.e., 1.7 mm, but the presence of 100 nM of Cu 2+ led to a much longer accumulation of PMPs, i.e., 3.2 mm, suggesting a more active and stable generation of •OH by the Cu 2+ /H 2 O 2 system [28]. Next, we tested the tolerance to water hardness by collecting water samples with different hardnesses.…”

Section: Tolerance To Environmental Interferencementioning

confidence: 95%

Visual Quantitation of Copper Ions Based on a Microfluidic Particle Dam Reflecting the Cu(II)-Catalyzed Oxidative Damage of DNA

Cui

Chen

2021

Biosensors

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Due to the use of copper water pipes and the discharge of industrial wastewater, contamination of copper ions in drinking water has become a severe hazard globally. To routinely check water safety on a daily basis, easy-to-use platforms for quantitative analysis of trace amounts of copper ions (Cu2+) in drinking water is needed. Here, we report microfluidic particle accumulation integrated with a Cu(II)-catalyzed Fenton reaction for visual and quantitative copper ion detection. Microparticles (MMPs) and polystyrene microparticles (PMPs) are connected via a single strand DNA, MB155. However, when Cu2+ is present, MB155 is cleaved by hydroxyl free radicals (•OH) produced from Cu2+/hydrogen peroxide (H2O2) Fenton reactions, causing an increased amount of free PMPs. To visually count them, the particle solution is loaded onto a microfluidic chip where free MMPs and MMPs–MB155–PMPs can be collected by the magnetic separator, while the free PMPs continue flowing until being accumulated at the particle dam. The results showed a good linear relationship between the trapping length of PMP accumulation and the Cu2+ concentration from 0 to 300 nM. A limit of detection (LOD) of 70.1 nM was achieved, which is approximately 449 times lower than the 2 × 103 μg·L−1 (~31.5 μM) required by the World Health Organization (WHO). Moreover, the results showed high selectivity and good tolerance to pH and hardness, indicating compatibility for detection in tap water, suggesting a potential platform for the routine monitoring of copper contamination in drinking water.

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Section: Tolerance To Environmental Interferencementioning

confidence: 95%