Quantification of human urinary exosomes by nanoparticle tracking analysis

Oosthuyzen, Wilna; Sime, Nicole E. L.; Ivy,; Turtle, Emma; Street, Jonathan M.; Pound, John D.; Bath, Louise; Webb, David J.; Gregory, Christopher D.; Bailey, Matthew; Dear, James W.

doi:10.1016/j.toxlet.2014.06.822

Cited by 16 publications

(31 citation statements)

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“…Flotillin‐1 was readily detected in the 20% iodixanol fraction (Fig. B) with a density of 1.09–1.12 g/mL in keeping with the previously reported density of exosomes of 1.16 g/mL in urine . A similar observation was seen for CD9 with maximal levels detected at 20% iodixanol (1.10 g/mL density).…”

Section: Resultssupporting

confidence: 89%

“…Recently, the release of vesicles, such as exosomes, into extracellular fluids, including urine, has been recognized, and roles in intercellular signalling, host defense and diagnosis have been suggested . Urinary extracellular vesicles (EVs) with the characteristics of exosomes have been described, as defined by electron microscopic appearance, nanoparticle tracking analysis (NTA), immunoblotting and proteomic analysis showing exosomal protein expression . Protocols for purification of EVs from urine have been refined .…”

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confidence: 99%

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Albuminuria is not associated with elevated urinary vesicle concentration but can confound nanoparticle tracking analysis

McNicholas

Michael

et al. 2017

Nephrology

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Section: Resultssupporting

confidence: 89%

mentioning

confidence: 99%

Albuminuria is not associated with elevated urinary vesicle concentration but can confound nanoparticle tracking analysis

McNicholas

Michael

et al. 2017

Nephrology

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“…Exosomes, firstly introduced by Trams et al in 1981, are small (30‐200 nm), lipid bilayer membrane vesicles which contain and transfer variety of bioactive lipids, proteins, mRNAs, and noncoding RNAs . Those extracellular vesicles can be identified from various body fluids including amniotic fluid, ascites, saliva, serum, plasma, urine, as well as the culture medium of cells . The roles of exosomes in the development of tumors have gained extensive attention in recent years, and increasing evidence reveals that exosomes are involved in many tumor‐related pathways, such as apoptosis, invasion, migration, and metastasis .…”

Section: Introductionmentioning

confidence: 99%

Exosomal Sonic Hedgehog derived from cancer‐associated fibroblasts promotes proliferation and migration of esophageal squamous cell carcinoma

Zhao

Guo

et al. 2020

Cancer Medicine

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Esophageal squamous cell carcinoma (ESCC) is one of the most common and aggressive malignancies in China. Cancer‐associated fibroblasts (CAFs) can actively communicate with and stimulate tumor cells, thereby contributing to the development and progression of tumors. Yet, whether CAFs‐derived exosomes have a role in the progression of ESCC is largely unknown. Here, we find that Sonic Hedgehog (SHH) is highly expressed in CAFs lysis solution, conditioned medium of cultured CAFs (CAF‐CM) and CAFs‐derived exosomes, and esophageal cancer cell lines educated by CAF‐CM and CAFs‐derived exosomes can improve their growth and migration abilities in vitro and in vivo. Besides, those effects can be partly neutralized by cyclopamine, inhibitor of the Hedgehog signaling pathway. Thus, our research elucidates the crucial role of CAFs‐derived exosomes in the growth and progression of ESCC, and may open up new avenues in the treatment of ESCC.

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“…; Oosthuyzen et al . ). Use of protease inhibitors to preserve samples has been described, but opinion remains inconsistent regarding their use (Zhou et al .…”

Section: Introductionmentioning

confidence: 97%

“…; Oosthuyzen et al . ). Calcium oxalate and amorphous calcium crystal precipitates can be present after thawing, macroscopically forming a cloudy sample.…”

Section: Introductionmentioning

confidence: 97%

Renal extracellular vesicles: from physiology to clinical application

Morrison

Bailey

Dear

2016

The Journal of Physiology

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Urinary extracellular vesicles (uEVs) are released from all regions of the kidney's nephron and from other cells that line the urinary tract. Extracellular vesicles retain proteomic and transcriptomic markers specific to their cell of origin and so represent a potential reservoir for kidney disease biomarker discovery. Exosomes, a subtype of uEVs, are distinguished from other vesicles by features related to their biogenesis within cells: mature multi-vesicular bodies fuse with the cellular membrane to liberate exosomes into the extracellular space. uEVs represent a novel cell signalling mechanism because they can be shuttled to a recipient cell and, through a number of proposed mechanisms, affect the recipient cell's proteome and function. Here we review the current evidence for uEV signalling along the nephron, their role in health and disease of the kidney, and their potential for clinical translation as biomarkers and therapeutics.

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Quantification of human urinary exosomes by nanoparticle tracking analysis

Cited by 16 publications

References 0 publications

Albuminuria is not associated with elevated urinary vesicle concentration but can confound nanoparticle tracking analysis

Albuminuria is not associated with elevated urinary vesicle concentration but can confound nanoparticle tracking analysis

Exosomal Sonic Hedgehog derived from cancer‐associated fibroblasts promotes proliferation and migration of esophageal squamous cell carcinoma

Renal extracellular vesicles: from physiology to clinical application

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