Quantification of human diamine oxidase

Boehm, Thomas; Pils, Sophie; Gludovacz, Elisabeth; Szoelloesi, Helen; Petroczi, Karin; Majdic, Otto; Quaroni, Andrea; Borth, Nicole; Valent, Peter; Jilma, Bernd

doi:10.1016/j.clinbiochem.2016.12.011

Cited by 34 publications

(51 citation statements)

References 35 publications

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“…The animal data are in remarkable agreement with human DAO concentrations during pregnancy, after heparin administration, and in non‐pregnant subjects (see Table and Ref. ). Schmutzler et al published a 30‐fold increase in plasma DAO activity in guinea pigs after induction of severe anaphylaxis.…”

Section: Discussionsupporting

confidence: 80%

“…The correct identification of individuals with MCAS might be increased. Except during pregnancy and after heparin administration, DAO concentrations are undetectable or if detectable on average only about 1‐2 ng/mL in healthy volunteers and mastocytosis patients (this study).…”

Section: Discussionmentioning

confidence: 51%

“…The development and characterization of the human DAO ELISA was recently published in detail . We tested two commercially available DAO ELISA kits but they were not reliable to measure human DAO …”

Section: Methodsmentioning

confidence: 99%

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Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients

Boehm

Reiter

Ristl

et al. 2019

Allergy

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Background Histaminolytic activity mediated by diamine oxidase (DAO) is present in plasma after induction of severe anaphylaxis in rats, guinea pigs, and rabbits. Heparin released during mast cell degranulation in the gastrointestinal tract might liberate DAO from heparin‐sensitive storage sites. DAO release during anaphylaxis has not been demonstrated in humans. Methods Plasma DAO, tryptase, and histamine concentrations of four severe anaphylaxis events were determined at multiple serial time points in two patients with systemic mastocytosis. The histamine degradation rates were measured in anaphylaxis samples and in pregnancy sera and plasma with comparable DAO concentrations. Results Mean DAO (132 ng/mL) and tryptase (304 ng/mL) concentrations increased 187‐ and 4.0‐fold, respectively, over baseline values (DAO 0.7 ng/mL, tryptase 76 ng/mL) during severe anaphylaxis. Under non‐anaphylaxis conditions, DAO concentrations were not elevated in 29 mastocytosis patients compared to healthy volunteers and there was no correlation between DAO and tryptase levels in mastocytosis patients. The histamine degradation rate of DAO in plasma from mastocytosis patients during anaphylaxis is severely compromised compared to DAO from pregnancy samples. Conclusion During severe anaphylaxis in mastocytosis patients, DAO is likely released from heparin‐sensitive gastrointestinal storage sites. The measured concentrations can degrade histamine, but DAO activity is compromised compared to pregnancy samples. For accurate histamine measurements during anaphylaxis, DAO inhibition is essential to inhibit further histamine degradation after blood withdrawal. Determination of DAO antigen levels might be of clinical value to improve the diagnosis of mast cell activation.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 51%

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Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients

Boehm

Reiter

Ristl

et al. 2019

Allergy

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“…The enzymatic activity of diamine oxidase (DAO, encoded by AOC1 ) is near the detection limit in serum or plasma of non-pregnant individuals but increases several hundred-fold during gestation 12 . The increase in activity has been confirmed by ELISA measurements of DAO antigen concentrations in sera and plasma of pregnant women 13 . DAO oxidizes polyamines including putrescine and spermidine and is the only extracellular enzyme capable of inactivating histamine in humans 13 , 14 .…”

Section: Introductionmentioning

confidence: 63%

“…The increase in activity has been confirmed by ELISA measurements of DAO antigen concentrations in sera and plasma of pregnant women 13 . DAO oxidizes polyamines including putrescine and spermidine and is the only extracellular enzyme capable of inactivating histamine in humans 13 , 14 . DAO shows the highest expression in intestine, kidney and placenta 15 .…”

Section: Introductionmentioning

confidence: 63%

Pregnancy-associated diamine oxidase originates from extravillous trophoblasts and is decreased in early-onset preeclampsia

Velicky

Windsperger

Petroczi

et al. 2018

Sci Rep

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Human extravillous trophoblast (EVT) invasion of the pregnant uterus constitutes a pivotal event for the establishment of the maternal-fetal interface. Compromised EVT function manifesting in inadequate arterial remodeling is associated with the severe pregnancy disorder early-onset preeclampsia (eoPE). Recent studies suggest that EVTs invade the entire uterine vasculature including arteries, veins and lymphatics in the first trimester of pregnancy. We therefore hypothesized that EVT-derived factors accumulate in the circulation of pregnant women early in gestation and may serve to predict eoPE. In contrast to published literature, we demonstrate that placenta-associated diamine oxidase (DAO) is not expressed by maternal decidual cells but solely by EVTs, especially when in close proximity to decidual vessels. Cultures of primary EVTs express and secret large amounts of bioactive DAO. ELISA measurements indicate a pregnancy-specific rise in maternal DAO plasma levels around gestational week (GW) 7 coinciding with vascular invasion of EVTs. Strikingly, DAO levels from eoPE cases were significantly lower (40%) compared to controls in the first trimester of pregnancy but revealed no difference at mid gestation. Furthermore, DAO-containing pregnancy plasma rapidly inactivates pathophysiologically relevant histamine levels. This study represents the first proof of concept suggesting EVT-specific signatures as diagnostic targets for the prediction of eoPE.

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Simple, sensitive and specific quantification of diamine oxidase activity in complex matrices using newly discovered fluorophores derived from natural substrates

et al. 2020

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Objective To measure diamine oxidase (DAO) activity with high sensitivity in complex matrices like plasma or tissue extracts radioactive putrescine or horseradish peroxidase (HRP)/hydrogen peroxide (H 2 O 2 ) coupling must be used. The use of radioactive material should be avoided and HRP/H 2 O 2 coupling is compromised by antioxidants. Methods and results Condensation of ortho-aminobenzaldehyde (oABA) with delta-1-pyrroline and delta-1-piperideine, the autocyclization products of the DAO-oxidized natural substrates putrescine and cadaverine, generates new quinazoline fluorophores with absorption and excitation maxima of 430 and 460 nm, respectively, and peak emission at 620 nm. Fluorescent-based detection limits are 20–40 times lower compared to absorption measurements. This assay can be used to measure DAO activity in human plasma after spiking recombinant human (rh)DAO, in rat plasma after intravenous rhDAO administration, in pregnancy plasma and in tissue extracts of DAO wild-type and knock-out mice. Using rat plasma the correlation between rhDAO activity and ELISA data is 99%. Human and rat plasma without DAO spiking and tissue extracts from DAO knock-out mice showed stable and low fluorescence in the presence of high substrate concentrations. Conclusions Incubation of DAO with the natural substrates putrescine and cadaverine and oABA generates novel fluorophores increasing the detection limit compared to absorption measurements at least tenfold. This simple, sensitive and specific assay allows the non-radioactive quantification of DAO activity in complex matrices like plasma and tissue extracts without interference by antioxidants. Electronic supplementary material The online version of this article (10.1007/s00011-020-01359-5) contains supplementary material, which is available to authorized users.

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Quantification of human diamine oxidase

Cited by 34 publications

References 35 publications

Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients

Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients

Pregnancy-associated diamine oxidase originates from extravillous trophoblasts and is decreased in early-onset preeclampsia

Simple, sensitive and specific quantification of diamine oxidase activity in complex matrices using newly discovered fluorophores derived from natural substrates

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