Quantification of human Aiolos splice variants by real-time PCR

Veistinen, Elli; Liippo, Jussi; Lassila, O.

doi:10.1016/s0022-1759(02)00370-8

Cited by 10 publications

(2 citation statements)

References 35 publications

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“…As a result, there is clearly a need to devise assays that can detect changes in expression levels of splice variants. Recently, real‐time PCR (5), standard microarrays (6), fiber‐optic microarrays (7, 8), and polony technology (14) have been utilized to meet this end. However, many of these approaches suffer from various limitations.…”

Section: Introductionmentioning

confidence: 99%

Detecting Changes in the Relative Expression of KRAS2 Splice Variants Using Polymerase Colonies

2004

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Because splice variants of a gene with multiple isoforms give rise to proteins with different functions, it seems plausible that changes in the expression levels of the splice variants could be a contributing factor to disease. In fact, recent examples in the literature clearly illustrate that altered expression levels of splice variants may play an important role in disease. Furthermore, these works demonstrate that changes in expression levels could potentially be used to (1) monitor disease progression, (2) diagnose disease, and/or (3) determine disease state. In this work an immobilized form of PCR, known as polony technology, was adapted to quantify the relative expression levels of splice variants. Specifically, the relative expression levels of the two splice variants of the oncogene K-ras, namely, K-RAS2A and K-RAS2B, were determined using polony technology.

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Section: Introductionmentioning

confidence: 99%

Detecting Changes in the Relative Expression of KRAS2 Splice Variants Using Polymerase Colonies

2004

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“…The assays then rely on exon-spanning probes to detect the two RNA species in essentially competitive reactions. Under these conditions, the large amount of unspliced template may interfere with probe binding and thus, make specific detection of the splice variant less accurate (Bustin, 2002;Kafert et al, 1999;Veistinen et al, 2002). The limit of detection for the unspliced transcript in these assays can be several orders of magnitude lower than that of the minor species, making the quantification of low-abundance transcripts difficult.…”

Section: Introductionmentioning

confidence: 99%