Quantification of Histone Acetyltransferase and Histone Deacetylase Transcripts During Early Bovine Embryo Development1

McGraw, Serge; Robert, Claude; Massicotte, Lyne; Sirard, Marc‐André

doi:10.1095/biolreprod.102.005991

Cited by 94 publications

(71 citation statements)

References 41 publications

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“…The peak expression at ZGA phase in this study shows a positive correlation to the lowest acetylation levels in rabbit embryo reported earlier [40]. In cattle, HDAC1 expression in the in vitro embryos was low until 8-cell stage, but increased eight to ten times at the blastocyst stage [52]. The presence of its protein in all the stages studied in both in vitro and in vivo embryos implies maternal contribution of HDAC1 prior to ZGA and its embryonic contribution post ZGA.…”

Section: Discussionsupporting

confidence: 69%

Candidate gene expression patterns in rabbit preimplantation embryos developed in vivo and in vitro

Gibence

Brahmasani

Mahesh

et al. 2014

J Assist Reprod Genet

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Purpose The levels and timing of expression of genes like BCLXL, HDAC1 and pluripotency marker genes namely, OCT4, SOX2, NANOG and KLF4 are known to influence preimplantation embryo development. Despite this information, precise understanding of their influence during preimplantation embryo development is lacking. The present study attempts to compare the expression of these genes in the in vivo and in vitro developed preimplantation embryos. Methods The in vivo and in vitro developed rabbit embryos collected at distinct developmental stages namely, pronuclear, 2 cell, 4 cell, 8 cell, 16 cell, Morula and blastocyst were compared at the transcriptional and translational levels using Real Time PCR and immunocytochemical studies respectively. Results The study establishes the altered levels of candidate genes at the transcriptional level and translational level with reference to the zygotic genome activation (ZGA) phase of embryo development in the in vivo and in vitro developed embryos. The expression of OCT4, KLF4, NANOG and SOX2 genes were higher in the in vitro developed embryos whereas and HDAC1 was lower. BCLXL expression had its peak at ZGA in in vivo developed embryos. Protein expression of all the candidate genes was observed in the embryos. BCLXL, KLF4 and NANOG exhibited diffused localisation whereas HDAC1, OCT4, and SOX2 exhibited nuclear localisation. Conclusions This study leads to conclude that BCLXL peak expression at the ZGA phase may be a requirement for embryo development. Further expression of all the candidate genes was influenced by ZGA phase of development at the transcript level, but not at the protein level.

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Section: Discussionsupporting

confidence: 69%

Candidate gene expression patterns in rabbit preimplantation embryos developed in vivo and in vitro

Gibence

Brahmasani

Mahesh

et al. 2014

J Assist Reprod Genet

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“…There have been some reports on the gene expression of histone deacetylases in embryos of different species. A bovine study showed that the expression of the Hdac1 gene is higher than that of the Hdac2 and Hdac3 genes in two-cell stage embryos (McGraw et al, 2003). There is no study on the effect of SHH or its agonists in an oocyte maturation medium on Hdac family gene expression, although it has been reported that adding Smoothened agonist (SAG), an SHH agonist, to the culture medium of cerebellar granule precursor (CGP) cells derived from medulloblastoma was accompanied by an increase in the expression and function of Hdac1, 2 and 3 genes (Lee et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Effect of purmorphamine on the mRNA expression of Sonic Hedgehog signaling downstream molecules in ovine embryo

2016

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Abstract. Sonic Hedgehog (SHH) is a signaling pathway mediated through a receptor system which seems to have effects on oocyte maturation and embryonic development. Purmorphamine is an SHH agonist that performs a crucial role in the regulation of the activity of SHH receptors and downstream transcription factors. The aim of this study was to analyze the effect of purmorphamine on the mRNA expression of SHH signaling downstream molecules (Patched1, Glioma-Associated Oncogene1, Smoothened, Histone Deacetylase1, Histone Deacetylase2 and Histone Deacetylase3) in ovine two-cell embryo. Ovaries were obtained from a slaughterhouse, and cumulus-oocyte complexes were aspirated and cultured in maturation media containing 0, 250 or 500 ng mL −1 purmorphamine. Then, oocytes were fertilized and cultured in a CR1 culture medium and after 24 h, two-cell embryos were collected for RNA extraction. Gene expression was evaluated by real-time polymerase chain reaction (PCR). Results indicated that in 250 ng mL −1 purmorphamine, Smo, Ptch1 and Hdac3 expression reduced, Hdac1 expression increased, and Gli1 and Hdac2 expression levels did not change. In 500 ng mL −1 purmorphamine, Gli1 and Smo transcripts increased, while Ptch1, Hdac2 and Hdac3 transcripts decreased. Regarding to the presence of SHH signaling molecules in two-cell embryos and their response to purmorphamine, it can be suggested that SHH signaling is probably active before embryonic genome activation in ovine embryos.

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“…The differential remethylation of the ICM also varies since both in the rabbit (Shi et al 2004) and in the human (Fulka et al 2004), DNA methylation is higher in the TE than in ICM. Variation in levels of histone deacetylases and histone acetyltransferases throughout bovine embryonic development have been reported (McGraw et al 2003(McGraw et al , 2007. McGraw et al (2003McGraw et al ( , 2007 described the temporal expression profile, during preimplantation embryo development, of 15 key regulators involved in RNA, DNA or histone methylation, chromatin modification or silencing, and transcription regulation; all were present to different degrees in the developmental stages tested, and they can be divided into three different groups depending on their respective mRNA profile.…”

Section: Preimplantation Embryo Programmingmentioning

confidence: 99%

“…Variation in levels of histone deacetylases and histone acetyltransferases throughout bovine embryonic development have been reported (McGraw et al 2003(McGraw et al , 2007. McGraw et al (2003McGraw et al ( , 2007 described the temporal expression profile, during preimplantation embryo development, of 15 key regulators involved in RNA, DNA or histone methylation, chromatin modification or silencing, and transcription regulation; all were present to different degrees in the developmental stages tested, and they can be divided into three different groups depending on their respective mRNA profile. More detailed comparative analysis of specific regions of the genome have yet to be carried out in order to understand the developmental consequences of these epigenetic reprogramming events, their consequences on gene expression reprogramming at EGA and on totipotency reprogramming.…”

Section: Preimplantation Embryo Programmingmentioning

confidence: 99%

Preimplantation embryo programming: transcription, epigenetics, and culture environment

Duranthon¹,

Watson²,

Lonergan³

2008

Reproduction

101

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Preimplantation development directs the formation of an implantation-or attachment-competent embryo so that metabolic interactions with the uterus can occur, pregnancy can be initiated, and fetal development can be sustained. The preimplantation embryo exhibits a form of autonomous development fueled by products provided by the oocyte and also from activation of the embryo's genome. Despite this autonomy, the preimplantation embryo is highly influenced by factors in the external environment and in extreme situations, such as those presented by embryo culture or nuclear transfer, the ability of the embryo to adapt to the changing environmental conditions or chromatin to become reprogrammed can exceed its own adaptive capacity, resulting in aberrant embryonic development. Nuclear transfer or embryo culture-induced influences not only affect implantation and establishment of pregnancy but also can extend to fetal and postnatal development and affect susceptibility to disease in later life. It is therefore critical to define the basic program controlling preimplantation development, and also to utilize nuclear transfer and embryo culture models so that we may design healthier environments for preimplantation embryos to thrive in and also minimize the potential for negative consequences during pregnancy and post-gestational life. In addition, it is necessary to couple gene expression analysis with the investigation of gene function so that effects on gene expression can be fully understood. The purpose of this short review is to highlight our knowledge of the mechanisms controlling preimplantation development and report how those mechanisms may be influenced by nuclear transfer and embryo culture.

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Quantification of Histone Acetyltransferase and Histone Deacetylase Transcripts During Early Bovine Embryo Development1

Cited by 94 publications

References 41 publications

Candidate gene expression patterns in rabbit preimplantation embryos developed in vivo and in vitro

Candidate gene expression patterns in rabbit preimplantation embryos developed in vivo and in vitro

Effect of purmorphamine on the mRNA expression of Sonic Hedgehog signaling downstream molecules in ovine embryo

Preimplantation embryo programming: transcription, epigenetics, and culture environment

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