Quantification of glyphosate and aminomethylphosphonic acid from microbiome reactor fluids

Fritz-Wallace, Katarina; Engelmann, Beatrice; Krause, Jannike Lea; Schäpe, Stephanie Serena; Pöppe, Judith; Herberth, Gunda; Rösler, Uwe; Jehmlich, Nico; Bergen, Martin von; Rolle-Kampczyk, Ulrike

doi:10.1002/rcm.8668

Cited by 10 publications

(8 citation statements)

References 44 publications

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“…Although it is not possible to define bacterial species as either ‘good’ or ‘bad’, Proteobacteria are generally regarded to have a negative influence on human health ( Shin et al, 2015 ). In a recent study using bioreactor systems inoculated with colonic bacteria from German Landrace pigs, glyphosate concentrations remained unchanged after a short two day exposure ( Fritz-Wallace et al, 2020 ). However, it remains unclear as to whether glyphosate metabolic pathways exist in the human gut microbiome.…”

Section: Discussionmentioning

confidence: 99%

Computational modelling provides insight into the effects of glyphosate on the shikimate pathway in the human gut microbiome

Mesnage

Antoniou

2020

Current Research in Toxicology

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The herbicide active ingredient glyphosate can affect the growth of microorganisms, which rely on the shikimate pathway for aromatic amino acid biosynthesis. However, it is uncertain whether glyphosate exposure could lead to perturbations in the population of human gut microbiota. We have addressed this knowledge gap by analysing publicly available datasets to provide new insights into possible effects of glyphosate on the human gut microbiome. Comparison of the abundance of the shikimate pathway in 734 paired metagenomes and metatranscriptomes indicated that most gut bacteria do not possess a complete shikimate pathway, and that this pathway is mostly transcriptionally inactive in the human gut microbiome. This suggests that gut bacteria are mostly aromatic amino acid auxotrophs and thus relatively resistant to a potential growth inhibition by glyphosate. As glyphosate blocking of the shikimate pathway is via inhibition of EPSPS, we classified E. coli EPSPS enzyme homologues as class I (sensitive to glyphosate) and class II (resistant to glyphosate). Among 44 subspecies reference genomes, accounting for 72% of the total assigned microbial abundance in 2144 human faecal metagenomes, 9 subspecies have class II EPSPS. The study of publicly available gut metagenomes also indicated that glyphosate might be degraded by some Proteobacteria in the human gut microbiome using the carbon–phosphorus lyase pathway. Overall, there is limited experimental evidence available for the effects of glyphosate on the human gut microbiome. Further investigations using more advanced molecular profiling techniques are needed to ascertain whether glyphosate and glyphosate-based herbicides can alter the function of the gut microbiome with consequent health implications.

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Section: Discussionmentioning

confidence: 99%

Computational modelling provides insight into the effects of glyphosate on the shikimate pathway in the human gut microbiome

Mesnage

Antoniou

2020

Current Research in Toxicology

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“…Since glyphosate is an organophosphate compound, this observation may originate from previously unknown biotransformation of glyphosate. In another bioreactor study investigating the porcine gut microbiota, it was found that AMPA was produced by the bacterial communities from the degradation of glyphosate (Fritz-Wallace et al, 2020). A more recent study of the metabolism of glyphosate in vitro by the use of human fecal suspension (HFS) samples found that biotransformation of glyphosate seems to be negligible in human gut microbiota from 15 healthy adult humans (Huch et al, 2021) Perhaps the most surprising metabolome changes we observed was the increase in levels of n3 and n6 long chain polyunsaturated fatty acids (PUFA) Some limited groups of bacteria can synthetize distinct unsaturated fatty acids such as arachidonic acid (20:4 n-6), eicosapentaenoic acid (20:5 n-3), and docosahexaenoic acid (22:6 n-3) (Okuyama et al 2007;Yoshida et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Alterations in infant gut microbiome composition and metabolism after exposure to glyphosate and Roundup and/or a spore-based formulation using the SHIME technology

et al. 2022

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Despite extensive research into the toxicology of the herbicide glyphosate, there are still major unknowns regarding its effects on the human gut microbiome. We describe the effects of glyphosate and a Roundup glyphosate-based herbicide on infant gut microbiota using SHIME technology. SHIME® microbiota culture was undertaken in the presence of a concentration of 100 mg/L glyphosate and the same glyphosate equivalent concentration of Roundup. Roundup and to a lesser extent glyphosate caused an increase in fermentation activity, resulting in acidification of the microbial environment. This was also reflected by an increase in lactate and acetate production concomitant to a decrease in the levels of propionate, valerate, caproate and butyrate. Ammonium production reflecting proteolytic activities was increased by Roundup exposure. Global metabolomics revealed large scale disturbances, including an increased abundance of long chain polyunsaturated fatty acids. Changes in bacterial composition measured by qPCR and 16S rRNA suggested that lactobacilli had their growth stimulated as a result of microenvironment acidification. Cotreatment with the spore-based probiotic formulation MegaSporeBiotic reverted some of the

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“…Quantification of 2 in this study was based on the previous methods with minor modifications 75 , 76 . Briefly, samples (1 mL) for 2 measurement were taken after 5-day bacterial culture and was centrifuged (10,000 rpm, 5 min, 4 °C).…”

Section: Methodsmentioning

confidence: 99%

Harnessing phosphonate antibiotics argolaphos biosynthesis enables a synthetic biology-based green synthesis of glyphosate

et al. 2022

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Glyphosate is a widely used herbicide with an annual production of more than one million tons globally. Current commercialized production processes of glyphosate are generally associated with manufacturing hazards and toxic wastes. Recently, many countries have strengthened environmental supervision and law enforcement on glyphosate manufacturing. Therefore, a green source of glyphosate is required. Here, we characterize the genes required for producing aminomethylphosphonate (AMP), one of the intermediates in the biosynthesis of the potent antibiotics argolaphos. We apply a synthetic biology strategy to improve AMP production in Streptomyces lividans, with fermentation titers of 52 mg L-1, a 500-fold improvement over the original strain. Furthermore, we develop an efficient and practical chemical process for converting AMP to glyphosate. Our findings highlight one greenness-driven alternative in the production of glyphosate.

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Quantification of glyphosate and aminomethylphosphonic acid from microbiome reactor fluids

Cited by 10 publications

References 44 publications

Computational modelling provides insight into the effects of glyphosate on the shikimate pathway in the human gut microbiome

Computational modelling provides insight into the effects of glyphosate on the shikimate pathway in the human gut microbiome

Alterations in infant gut microbiome composition and metabolism after exposure to glyphosate and Roundup and/or a spore-based formulation using the SHIME technology

Harnessing phosphonate antibiotics argolaphos biosynthesis enables a synthetic biology-based green synthesis of glyphosate

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