Quantification of folpet and phthalimide in tea and herbal infusions by LC– high-resolution MS and GC–MS/MS

Huertas-Pérez, José F.; Ernest, Marion; Badoud, Flavia

doi:10.1080/19440049.2018.1555379

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…GC-MS consists of two analytical techniques where GC splits the component from the mixture, and MS analyzes each of the components separately [ 28 ]. In addition, compounds that are small, adequately volatile, and thermostable in the GC environment can be easily analyzed by GC–MS [ 29 ]. Previously, GC-MS analysis on the hexane extract of Kenaf leaves and seed manifested 13 and 10 phytoconstituents, respectively [ 12 ].…”

Section: Resultsmentioning

confidence: 99%

Kenaf (Hibiscus cannabinus L.) Leaves and Seed as a Potential Source of the Bioactive Compounds: Effects of Various Extraction Solvents on Biological Properties

Adnan

Azad

et al. 2020

Life

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Hibiscus cannabinus (Kenaf) is a potential source of bioactive constituents and natural antioxidant. The current study determined the impact of various solvents on extraction yield, recovery of polyphenol and flavonoid, antioxidant, anticancer, and antibacterial properties of Kenaf leaves and seed. The powder of leaves and seed was separately extracted with n-hexane, ethyl acetate, ethanol, and water solvent. Among them, the ethanol extract of leaves and seed showed the highest extraction yield, and their GC-MS analysis revealed a total of 55 and 14 bioactive compounds, respectively. The total polyphenols (TP) and flavonoids (TF) content were quantified by a spectrophotometric technique where water extracts displayed a noteworthy amount of TP and TF content compared to other extracts. A similar demonstration was noticed in antioxidant activity, evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) and hydrogen peroxide scavenging capacity. In addition, cytotoxicity and anti-lung cancer activity were identified against mouse embryonic fibroblast (NIH3T3) and human lung cancer (A549) cells. All extracts of leaves and seed were observed as non-toxic to the NIH3T3 cells, but slight toxicity was expressed by n-hexane extracts at the optimum dose (1000 µg/mL) of treatment. In parallel, n-hexane and ethanol extracts (leaves and seed) exposed promising anti-lung cancer activity at the same concentration. Furthermore, antibacterial activity was assessed using disc diffusion assay, and seed extracts exhibited a significant inhibition zone against Gram-positive and Gram-negative microorganisms. Overall, Kenaf seed extracted with polar solvents was found very potent in terms of important bioactive compounds and pharmacological aspects, which can be an excellent biological matrix of natural antioxidants.

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Section: Resultsmentioning

confidence: 99%

Kenaf (Hibiscus cannabinus L.) Leaves and Seed as a Potential Source of the Bioactive Compounds: Effects of Various Extraction Solvents on Biological Properties

Adnan

Azad

et al. 2020

Life

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“…GC-MS, coupled with appropriate detection systems is an invaluable tool for the separation and identification of the components of complex, volatile mixtures [14]. Many plant secondary metabolites are sufficiently small, adequately volatile, and thermostable in the GC environment to be easily analyzed by GC-MS [15]. In addition to the phytochemical investigation, we aimed to investigate the known therapeutic applications of the plant through a combination of in vivo (antidiarrheal and anti-inflammatory), in vitro (anthelmintic and antibacterial) and in silico (molecular docking, ADME/T and PASS) analyses.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Biological Activities and Characterization of Bioactive Constituents of Ophiorrhiza rugosa var. prostrata (D.Don) & Mondal Leaves through In Vivo, In Vitro, and In Silico Approaches

et al. 2019

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Ophiorrhiza rugosa var. prostrata is one of the most frequently used ethnomedicinal plants by the indigenous communities of Bangladesh. This study was designed to investigate the antidiarrheal, anti-inflammatory, anthelmintic and antibacterial activities of the ethanol extract of O. rugosa leaves (EEOR). The leaves were extracted with ethanol and subjected to in vivo antidiarrheal screening using the castor oil-induced diarrhea, enteropooling, and gastrointestinal transit models. Anti-inflammatory efficacy was evaluated using the histamine-induced paw edema test. In parallel, in vitro anthelmintic and antibacterial activities were evaluated using the aquatic worm and disc diffusion assays respectively. In all three diarrheal models, EEOR (100, 200 and 400 mg/kg) showed obvious inhibition of diarrheal stool frequency, reduction of the volume and weight of the intestinal contents, and significant inhibition of intestinal motility. Also, EEOR manifested dose-dependent anti-inflammatory activity. Anthelmintic action was deemed significant (P < 0.001) with respect to the onset of paralysis and helminth death. EEOR also resulted in strong zones of inhibition when tested against both Gram-positive and Gram-negative bacteria. GC-MS analysis identified 30 compounds within EEOR, and of these, 13 compounds documented as bioactive showed good binding affinities to M3 muscarinic acetylcholine, 5-HT3, tubulin and GlcN-6-P synthase protein targets in molecular docking experiments. Additionally, ADME/T and PASS analyses revealed their drug-likeness, likely safety upon consumption and possible pharmacological activities. In conclusion, our findings scientifically support the ethnomedicinal use and value of this plant, which may provide a potential source for future development of medicines.

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“…In the field of the forensic science the GC-MS technique pre-treatment process is being used which is extremely simple when compared to the conventional pre-treatment process that is less complex in nature; this process is called as headspace solid-phase microextraction (HS-SPME) and together with GC-MS it is said to be HS-SPME-GC/MS. Different detection techniques are used in coupling with the GC-MS techniques that are electron capture detection (ECD) and electron ionization (EI) with single quadrapole MS and/or triple quadrapole MS (MS-MS) [56,57].…”

Section: Lc-ms For the Quality Control Of Botanical Herbsmentioning

confidence: 99%

Quality control of the traditional herbs and herbal products: a review

2020

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Background Herbal medicinal material and product need is increasing, and with this increase in the need, it is very much an essential requirement to maintain the quality of them. Main body The quality of the herbals is altered by various physical, chemical, and geographical aspects which contribute to the quality of these materials. Apart from that, adulteration is also an increasing concern when it comes to herbal material quality. Various chemical and phytochemical test, analytical techniques, and hyphenated analytical techniques are used for determining the quality aspects of the herbal materials in the herbal pharmaceuticals. Conclusion These techniques can be used as quality control tool in assessing the quality of herbal materials and herbal pharmaceuticals.

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Quantification of folpet and phthalimide in tea and herbal infusions by LC– high-resolution MS and GC–MS/MS

Cited by 8 publications

References 21 publications

Kenaf (Hibiscus cannabinus L.) Leaves and Seed as a Potential Source of the Bioactive Compounds: Effects of Various Extraction Solvents on Biological Properties

Kenaf (Hibiscus cannabinus L.) Leaves and Seed as a Potential Source of the Bioactive Compounds: Effects of Various Extraction Solvents on Biological Properties

Investigation of the Biological Activities and Characterization of Bioactive Constituents of Ophiorrhiza rugosa var. prostrata (D.Don) & Mondal Leaves through In Vivo, In Vitro, and In Silico Approaches

Quality control of the traditional herbs and herbal products: a review

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