Quantification of folate metabolites in serum using ultraperformance liquid chromatography tandem mass spectrometry

Wang, Xiuwei; Zhang, Ting; Zhao, Xin; Guan, Zhiyong; Wang, Zhen; Zhu, Zhiqiang; Xie, Qijun; Wang, Jianhua; Niu, Bo

doi:10.1016/j.jchromb.2014.05.023

Cited by 30 publications

(30 citation statements)

References 31 publications

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“…Reverse phase chromatography (RPC) is the first choice for the separation of the modified pteridinic species. Previous work from Wang et al described a LC‐MS/MS method for the quantification of FA, 5MTHF, and FO in human plasma . Parallel research, reported in Owens et al and Zayed et al, presented alternative methods for deciphering the concentration of FA, 5MTHF, and FO in whole human blood …”

Section: Introductionmentioning

confidence: 92%

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Comparison of ESI‐MS/MS and APCI‐MS methods for the quantification of folic acid analogs in C. elegans

et al. 2019

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Folic acid (FA) plays a vital role in central metabolism, including the one carbon cycle, nucleotide, and amino acid biosynthesis. The development of sensitive, accurate analytical methods to measure FA intermediates in tissues is critical to understand their biological roles in diverse physiological and pathological contexts. Here, we developed a highly sensitive method for the simultaneous quantification of FA intermediates in the nematode Caenorhabditis elegans as a model to dissect metabolic networks. The method was further validated by analyzing the worm folate pool upon RNAi knockdown of the dihydrofolate reductase gene dhfr‐1. Comparative mass spectrometry behavior of the FA analogs using two different ion sources, electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI), revealed ESI‐MS/MS to be more sensitive, but APCI‐MS provided more detailed structure inferences, which can elucidate chemical investigation and synthesis of FA analogs. Finally, we report on the use of in vitro oxidation coupled with high‐resolution mass spectrometry as a tool to discover new endogenous FA derivatives in the nematode.

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Section: Introductionmentioning

confidence: 92%

“…Previous work from Wang et al described a LC-MS/MS method for the quantification of FA, 5MTHF, and FO in human plasma. 17 FO in whole human blood. 18,19 Electrospray ionization (ESI) is the primary ionization technique for ionizing polar organic compounds.…”

mentioning

confidence: 98%

Comparison of ESI‐MS/MS and APCI‐MS methods for the quantification of folic acid analogs in C. elegans

et al. 2019

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“…In addition, the methods including microorganism method, radioimmunoassay, high‐performance liquid chromatography (HPLC) and electroanalysis have been reported for codetermination of FA and its metabolites, whereas the complicated sample treatment or the poor sensitivity is a shortcoming for hindering their further application. Hence, high‐performance liquid chromatography–tandem mass spectrometry (HPLC‐MS/MS) with high specificity and sensitivity is applied to determinate the FA and its metabolites in plasma …”

Section: Introductionmentioning

confidence: 99%

Folic acid and its metabolite codetermination for pharmacokinetics with circadian rhythms and evaluation of oral bioavailability

Meng

Zhang

Wang

et al. 2019

Journal of Pharmacy and Pharmacology

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Objectives Pharmacokinetics of vitamins is still a challenge. In this study, folic acid (FA) was used as a model drug and aimed at investigating a reliable method for its detailed pharmacokinetic evaluations. Methods An high‐performance liquid chromatography–tandem mass spectrometry method was developed and performed to determinate the FA and 5‐methyltetrahydrofolic acid (5‐methylTHF) simultaneously, which was applied to characterize the circadian rhythms as well as the pharmacokinetics of different preparations. Key findings The plasma concentration of 5‐methylTHF in fasted state was twofold higher than that in fed state. The circadian rhythms were studied before the pharmacokinetics and revealed that free FA was almost undetected in blank plasma, while 5‐methylTHF had a slight decrement at 12:00. Hence, the pharmacokinetics of FA was conducted and showed that the administration of FA solution resulted in enhancing bioavailability of 5‐methylTHF comparing with FA raw material suspension, whereas the free FA level in plasma was similar. The mechanism could be that FA was rapidly metabolized to 5‐methylTHF in intestinal epithelial cell after absorption, which revealed that intestinal metabolism would affect its bioavailability. Conclusion A suitable method was established considering the baseline level, circadian rhythms and intestinal metabolism to investigate the pharmacokinetics of FA for guiding the further research of vitamins.

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“…Folate analysis using UPLC has also been reported to overcome timeconsuming analysis using conventional analytical columns. 23 In this study, we have developed an analytical method for the accurate determination of folic acid in human serum using ID-UPLC/MS as a higher-order reference method. As only a few studies on the development of ID-UPLC/MS methods have been reported, further elaborate evaluation and improvements are still needed for faster and more accurate analysis of folic acid.…”

Section: Introductionmentioning

confidence: 99%

Quantification of Folic Acid in Human Serum Using Isotope Dilution Ultra‐High‐Pressure Liquid Chromatography/Mass Spectrometry

Hyung

Lee

Seo³

et al. 2018

Bulletin Korean Chem Soc

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Analytical method for an accurate determination of folic acid in human serum has been established using isotope dilution ultra-high-pressure liquid chromatography/mass spectrometry (ID-UPLC/MS). Sample extract was prepared from 0.5 mL of human serum spiked with 13 C 5 -folic acid solution and then LC-MS/ MS experiment was performed on multiple reaction monitoring mode for 20 min. The method was tested for repeatability using homogeneous human serum and it showed good repeatability with relative standard deviation of 1.8%. Further evaluation was carried out through NIST SRM 1955 samples (level I, II, and III) and the measurement results were in good agreement with the certified values with good reproducibility over two periods. It was also revealed that the obtained relative expanded uncertainty was 12% which is much smaller than that of previously reported methods, indicating that this method is very reliable and accurate for determining the content of folic acid in human serum.

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Quantification of folate metabolites in serum using ultraperformance liquid chromatography tandem mass spectrometry

Cited by 30 publications

References 31 publications

Comparison of ESI‐MS/MS and APCI‐MS methods for the quantification of folic acid analogs in C. elegans

Comparison of ESI‐MS/MS and APCI‐MS methods for the quantification of folic acid analogs in C. elegans

Folic acid and its metabolite codetermination for pharmacokinetics with circadian rhythms and evaluation of oral bioavailability

Quantification of Folic Acid in Human Serum Using Isotope Dilution Ultra‐High‐Pressure Liquid Chromatography/Mass Spectrometry

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