Quantification of fluoxetine and norfluoxetine serum levels by reversed-phase high-performance liquid chromatography with ultraviolet detection

Holladay, John W.; Dewey, Michael J.; Yoo, Sun Dong

doi:10.1016/s0378-4347(97)00470-2

Cited by 25 publications

(13 citation statements)

References 17 publications

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“…Mice of three different groups of age received fluoxetine daily in a dosage leading to clinically relevant plasma concentrations over a long period of time to mimic chronic treatments administered to human patients. 32,33 In young animals, we could confirm that fluoxetine promotes neurogenesis, as a result of an enhancement of survival of newly generated cells and a cell fate shift towards neuronal phenotypes. Fluoxetine, however, neither enhanced the proliferation rate of progenitors in the hippocampus of young animals nor in old animals.…”

Section: Discussionmentioning

confidence: 65%

“…In mice, this dosage has been reported to yield fluoxetine serum levels, which are comparable to those observed in patients receiving fluoxetine as antidepressant medication. 32,33 Control animals received volume-matched injections of PBS. For the analysis of cell proliferation, mice received a single injection of BrdU at 200 mg kg À1 of body weight on day 28 and 2 h later mice were perfused under deep anesthesia with a pH 7.4 phosphate-buffered 4% paraformaldehyde solution.…”

Section: Animalsmentioning

confidence: 99%

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Ageing abolishes the effects of fluoxetine on neurogenesis

et al. 2009

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Depression constitutes a widespread condition observed in elderly patients. Recently, it was found that several drugs employed in therapies against depression stimulate hippocampal neurogenesis in young rodents and nonhuman primates. As the rate of neurogenesis is dramatically reduced during ageing, we examined the influences of ageing on neurogenic actions of antidepressants. We tested the impact of fluoxetine, a broadly used antidepressant, on hippocampal neurogenesis in mice of three different age groups (100, 200 and over 400 days of age). Proliferation and survival rate of newly generated cells, as well as the percentage of cells that acquired a neuronal phenotype were analyzed in the hippocampus of mice that received fluoxetine daily in a chronic manner. Surprisingly, the action of fluoxetine on neurogenesis was decreasing as a function of age and was only significant in young animals. Hence, fluoxetine increased survival and the frequency of neuronal marker expression in newly generated cells of the hippocampus in the young adult group (that is 100 days of age) only. No significant effects on neurogenesis could be detected in fluoxetine-treated adult and elderly mice (200 and over 400 days of age). The data indicate that the action of fluoxetine on neurogenesis is highly dependent on the age of the treated individual. Although the function of neurogenesis in the clinical manifestation of depression is currently a matter of speculation, this study clearly shows that the therapeutic effects of antidepressants in elderly patients are not mediated by neurogenesis modulation.

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Section: Discussionmentioning

confidence: 65%

Section: Animalsmentioning

confidence: 99%

Ageing abolishes the effects of fluoxetine on neurogenesis

et al. 2009

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“…The most widely used methods involve high-performance liquid chromatography (HPLC) with UV detection [4,11,12,[14][15][16][17][18]. FL/NORFL levels can also be measured in biological samples using gas chromatography (GC) coupled with MS [21,22], flame ionization [23], electron capture [24,25] or nitrogen phosphorus detection [26].…”

Section: Introductionmentioning

confidence: 99%

Comparison of liquid chromatography with fluorescence detection to liquid chromatography–mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasma

Kovačević

Pokrajac

Miljković

et al. 2006

Journal of Chromatography B

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“…Most previously reported HPLC-UV methods involved multiple step liquid-liquid extraction (LLE) procedures for sample clean-up to remove interfering materials [16][17][18][19][20][21]. These conventional LLE techniques are labour intensive, time-consuming and difficult for batch processing and automation for routine analysis.…”

Section: Introductionmentioning

confidence: 99%