Quantification of fatty acids as methyl esters and phospholipids in cheese samples after separation of triacylglycerides and phospholipids

Hauff, Simone; Vetter, Walter

doi:10.1016/j.aca.2009.01.056

Cited by 31 publications

(39 citation statements)

References 35 publications

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“…Furthermore, the method included m/z 88 and m/z 101 for the determination of the ethyl esters used as internal standards [13,14]. Identification and quantification of the IS d 70 -DSPC was based on m/z 77 and m/z 91 [10,12]. Duplicates were analyzed for fatty acids in both NL and PL and mean values will be reported below.…”

Section: Gas Chromatography In Combination With Mass Spectrometry (Gcmentioning

confidence: 99%

“…Neutral and polar lipids were separated on deactivated silica (20%, w/w) in a Pasteur pipette (0.6 cm diameter) [10]. In brief, the column was pre-conditioned (3 mL ethyl acetate/ cyclohexane, 54:46 w/w).…”

Section: Separation Of Phospholipids From Neutral Lipids By Spementioning

confidence: 99%

“…The PL were eluted with 20 mL ethyl acetate/methanol (1:1, v/v), 20 mL methanol, and 20 mL methanol/water (98:2, v/v) into one flask (Fraction 2). The NL and PL fractions were concentrated separately by rotary evaporation to $2 mL, and then blown down with N 2 to dryness [10]. The PL content was calculated by the PC equivalent [10].…”

Section: Separation Of Phospholipids From Neutral Lipids By Spementioning

confidence: 99%

“…The NL and PL fractions were concentrated separately by rotary evaporation to $2 mL, and then blown down with N 2 to dryness [10]. The PL content was calculated by the PC equivalent [10].…”

Section: Separation Of Phospholipids From Neutral Lipids By Spementioning

confidence: 99%

“…The methods of Weichbrodt et al [5] and Hauff and Vetter [10] were used with the following modifications. We used 11 mL cells instead of larger ones (due to the low sample amounts) and we reduced the amount of the more non-polar solvent required for extraction of the polar lipids.…”

Section: Accelerated Solvent Extractionmentioning

confidence: 99%

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Fatty acids and polar lipid content of cheese and mould‐contaminated cheese

Schlosser¹,

Vetter²

2011

Euro J Lipid Sci & Tech

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Unintended infestation of food with viable mould irreversibly leads to spoilage of food. However, little data existed on the fatty acid composition and polar lipid content of mould grown on cheese and the potential influence of these parameters in mould-infested cheese. In this study, we thus focused on the fatty acids composition and polar lipid content of cheese, mould isolated from mould-infested cheese, as well as inoculation of cheese with cultures of isolated moulds. For this purpose, we stored three cheese samples (gouda, brown cheese, and parmesan) over extended periods in order to force mould infestation from air. The moulds grown on gouda and brown cheese were identified as Penicillium brevicompactum and Penicillium roqueforti, respectively, while a mixed culture was obtained from parmesan. From these contaminated samples, mould spores were isolated and cultivated. Finally, the (pure) cheeses were inoculated with mould spores. Three subsamples, (1) cheeses, (2) moulds isolated from cheese, and (3) cheeses infested with the isolated moulds were studied with regards to their fatty acid distribution in the neutral and the polar lipids. The different amounts of polar lipids of cheese (0.39-2%), moulds (11-30.7%), and mould-infested cheese (1.5-5%) allowed calculating the mould content of the mouldinfested cheeses.

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Section: Gas Chromatography In Combination With Mass Spectrometry (Gcmentioning

confidence: 99%

Section: Separation Of Phospholipids From Neutral Lipids By Spementioning

confidence: 99%

Section: Separation Of Phospholipids From Neutral Lipids By Spementioning

confidence: 99%

“…The NL and PL fractions were concentrated separately by rotary evaporation to $2 mL, and then blown down with N 2 to dryness [10]. The PL content was calculated by the PC equivalent [10].…”

Section: Separation Of Phospholipids From Neutral Lipids By Spementioning

confidence: 99%

Section: Accelerated Solvent Extractionmentioning

confidence: 99%

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