Quantification of effective exoelectrogens by most probable number (MPN) in a microbial fuel cell

Heidrich, Elizabeth S.; Curtis, Thomas P.; Woodcock, Stephen; Dolfing, Jan

doi:10.1016/j.biortech.2016.06.066

Cited by 29 publications

(14 citation statements)

References 21 publications

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“…The substrates were acetate, the most commonly used laboratory feed, and sterilised real wastewater. The two different inocula were raw influent wastewater also taken from this site, which has been shown to contain 17 effective electrogens per ml [21], and Arctic soil (see below) which could potentially have more psychrophilic bacteria.…”

Section: Experimental Designmentioning

confidence: 99%

“…Autoclaved acetate media [35] containing 1 g/L sodium acetate was compared to wastewater taken from the Cramlington wastewater treatment site (Northumbrian Water Ltd., UK), which was UV sterilised prior to use [20,21]. The cathode chamber was filled with 50 mM pH 7 sodium phosphate buffer, conductivity 8.2 mS/cm.…”

Section: Media and Inoculamentioning

confidence: 99%

“…The presence of effective electrogens in wastewater is low, in the order of 17 cells per ml [21]. An appropriate choice of inoculum might influence performance in general, and temperature sensitivity in particular.…”

Section: Introductionmentioning

confidence: 99%

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Temperature, inocula and substrate: Contrasting electroactive consortia, diversity and performance in microbial fuel cells

Heidrich

Dolfing

Wade

et al. 2018

Bioelectrochemistry

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The factors that affect microbial community assembly and its effects on the performance of bioelectrochemical systems are poorly understood. Sixteen microbial fuel cell (MFC) reactors were set up to test the importance of inoculum, temperature and substrate: Arctic soil versus wastewater as inoculum; warm (26.5°C) versus cold (7.5°C) temperature; and acetate versus wastewater as substrate. Substrate was the dominant factor in determining performance and diversity: unexpectedly the simple electrogenic substrate delivered a higher diversity than a complex wastewater. Furthermore, in acetate fed reactors, diversity did not correlate with performance, yet in wastewater fed ones it did, with greater diversity sustaining higher power densities and coulombic efficiencies. Temperature had only a minor effect on power density, (Q 10 : 2 and 1.2 for acetate and wastewater respectively): this is surprising given the well-known temperature sensitivity of anaerobic bioreactors. Reactors were able to operate at low temperature with real wastewater without the need for specialised inocula; it is speculated that MFC biofilms may have a self-heating effect. Importantly, the warm acetate fed reactors in this study did not act as direct model for cold wastewater fed systems. Application of this technology will encompass use of real wastewater at ambient temperatures.

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Section: Experimental Designmentioning

confidence: 99%

Section: Media and Inoculamentioning

confidence: 99%

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Temperature, inocula and substrate: Contrasting electroactive consortia, diversity and performance in microbial fuel cells

Heidrich

Dolfing

Wade

et al. 2018

Bioelectrochemistry

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“…The CP technique of MFCs uses the advantage of the microbial properties of a living microorganisms in which it is potentially able to produce electrons and therefore fulfill the requirement for CP in corrosion prevention system. The system uses the benefit of bacteria or microorganism presents in the wastewater that are able to generate electrons [1].…”

Section: Introductionmentioning

confidence: 99%

Utilization of Wastewater for Corrosion Prevention of Carbon Steel Pipe Using Single Chamber Microbial Fuel Cells

Suhaili¹,

Samsudin²

2018

Environ. ecosyst. sci.

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This study applies a concept Microbial Fuel Cells (MFCs) in a biological cathodic protection (CP) system. The biological CP uses the microbial properties of living microorganisms which are able to produce electrons and suffice the requirement for CP in corrosion prevention system. The electrons produce by the bacteria will be diffuse and transfer to the surface area of graphite which is use as anode. The objective of MFCs for CP is to achieve an optimum value of electro potential produce by bacteria inoculated in MFCs system as preventive measures against corrosion occurred in commonly used carbon steel pipeline. The purpose of the study is to identify the wastewater derived from industry, estuary and dairy farm animal that can give the nearest optimum value of -850 mV for CP. Prior to the objective, three different types of waste is choose as samples in this study which are palm oil mill effluent (POME), estuary water and goat's feces. Wastewater such as POME and estuary water is stored in a freezer at the temperature below 4oC. Meanwhile goat's feces is soak in distill water for 24 hours before it is place in a freezer. After the collection of wastewater, a proximity analysis such as pH, conductivity, salinity, COD and TSS is conducted to determine the quality of the wastewater. The 4 liter of wastewater is place in a rig and 40g of sodium acetate as substrate is mix so that it will act as medium for bacteria growth in a single chamber MFCs. The backfilling for the experiment is sand with sand to wastewater is 2L and 4L respectively. The anode (graphite) will undergoes treatment to remove contamination by soaking it in a 100% ethanol for 30 minutes and then soak in 1.0 M HCl for one hour. The electro potential reading is record periodically in hourly basis using electrical multi meter for each source of wastewater and correspondent current density and power density will be determined. In the experiment, three samples is used to represents the population of each type of wastewater in order to have accurate reading and more reliable results during analysis. A statistical analysis is use for the purpose of fulfilling the objective of the study. The result of study shows that wastewater from goat's feces gives the lowest rate of corrosion which is 1.18 x 10-6 kg/h and followed by estuary water and palm oil mill effluent of 4 x 10-6 kg/h and 7.18 x 10-6 kg/h respectively. It is also found that the wastewater from goat's feces achieved the highest mean negative potential of -578.07 mV while standard cathodic protection is -850 mV.

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“…Despite the advantages of culture-dependent techniques, including low cost and the potential to combine with other methods, the availability of culture-based methods for studies of environmental microbes gives a highly restricted view of microbial community structure in environmental ecosystems (Mahmod, 2014;Heidrich et al, 2016). Hence, culture-based approaches are not appropriate for comprehensive studies of the diversity and abundance of ARGs as well as the incidence of MGEs in WWTPs (Wang et al, 2013).…”

mentioning

confidence: 99%

Antimicrobial Resistance Genes and Wastewater Treatment

Mohammadali

Davies

2017

Antimicrobial Resistance in Wastewater Treatment Processes

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Quantification of effective exoelectrogens by most probable number (MPN) in a microbial fuel cell

Cited by 29 publications

References 21 publications

Temperature, inocula and substrate: Contrasting electroactive consortia, diversity and performance in microbial fuel cells

Temperature, inocula and substrate: Contrasting electroactive consortia, diversity and performance in microbial fuel cells

Utilization of Wastewater for Corrosion Prevention of Carbon Steel Pipe Using Single Chamber Microbial Fuel Cells

Antimicrobial Resistance Genes and Wastewater Treatment

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