Abstract:SUMMARY: A procedure is described for measuring dome morphologic parameters of LLC-PK 1 cells in culture. The method is based on an automated image analysis system directly connected to a stereornicroscope, combined with a stable staining technique of domes. Using well-defined and standardized culture conditions, and taking full advantage of video image potential, we have studied dome evolution during 7 days in culture. Moreover, after measuring the total surface of domes and the number of domes after a treatm… Show more
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