Quantification of DNA repair capacity in whole blood of patients with head and neck cancer and healthy donors by comet assay

Saha, Daniel T.; Davidson, Bruce J.; Wang, Antai; Pollock, Allison J.; Orden, Roy A.; Goldman, Radoslav

doi:10.1016/j.mrgentox.2007.10.004

Cited by 26 publications

(19 citation statements)

References 29 publications

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“…The study of Schmezer et al did not detect any significant difference between the levels of baseline DNA damage in lymphocytes of lung cancer patients and healthy individuals but found an increased sensitivity of lymphocytes to bleomycin and decreased DNA repair capacity in cancer patients [20]. The deficient DNA repair in lymphocytes of lung, head and neck cancer patients also has been shown by other researchers [4, 5, 8, 9]. It should be mentioned that the concentration of bleomycin (20 μg/ml) recommended by Schmezer et al, which we also used in our study, caused DNA damage at saturation level of the assay.…”

Section: Discussionmentioning

confidence: 82%

DNA repair deficiency in peripheral blood lymphocytes of endometrial cancer patients with a family history of cancer

et al. 2014

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BackgroundIndividual susceptibility to endogenous and/or exogenous DNA damage depends on DNA repair efficiency and can be evaluated using the comet assay with bleomycin as genotoxic agent. The aim of the study was to evaluate baseline and bleomycin-induced DNA damage and DNA repair capacity in peripheral blood lymphocytes (PBLs) of endometrial cancer (EC) patients considering a family history of cancer.MethodsDNA damage was analyzed in PBLs of 45 EC patients compared to a control group of 10 healthy women, using the comet assay. The level of DNA damage was determined by the% tail DNA.ResultsThe level of baseline DNA damage in PBLs of EC patients was significantly higher (% DNA in tail 9.31 ± 15.32) than in healthy women (% DNA in tail 3.41 ± 4.71) (P <0.01). PBLs of EC patients repaired less bleomycin-induced DNA damage (removed% DNA in tail 63.94 ± 20.92) than PBLs of healthy individuals (removed% DNA in tail 80.24 ± 3.03) (P <0.001). Efficiency of DNA repair in PBLs of EC patients depended on the family history of cancer. The amount of restored damaged DNA was significantly lower (removed% DNA in tail 36.24 ± 14.05%) in EC patients with a family history of cancer compared to patients with sporadic EC (removed% DNA in tail 64.91 ± 19.36%) (P <0.004).ConclusionsLymphocytes of EC patients are characterized by an increased basal level of DNA damage as well as deficiency in DNA repair. DNA repair is less efficient in PBLs of EC patients with a family history of cancer compared to patients with sporadic cancer.

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Section: Discussionmentioning

confidence: 82%

DNA repair deficiency in peripheral blood lymphocytes of endometrial cancer patients with a family history of cancer

et al. 2014

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“…Results of Saha et al (2008) also showed a slower DNA repair efficiency in HNSCC patients in comparison to healthy donors, measured by alkaline comet assay. The repair was slightly higher in controls compared with HNSCC patients who were matched based on age and smoking status.…”

Section: Discussionmentioning

confidence: 91%

DNA Damage and Oxidant-Antioxidant Status in Blood of Patients with Head and Neck Cancer

MilonskiJaroslaw

Zielinska-BlizniewskaHanna

Olszewski

et al. 2015

DNA and Cell Biology

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Oxidative stress induces a cellular redox imbalance that has been found to be present in various cancer cells, and overproduction of free radicals may be related to oncogenic stimulation. We investigated the activity of the following antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) concentrations in blood of patients with head and neck squamous cell carcinoma (HNSCC) compared with the control group. A comet assay was used to assess DNA damage. A nonsignificant increase of MDA and a decrease of SOD, CAT, and GPx (p>0.05) were seen in HNSCC patients compared with controls. It was found that the level of oxidative DNA damage in HNSCC patients was significantly higher compared with the control group (p ≤ 0.001). Our observations suggest that HNSSC patients may represent an impaired antioxidant defense system, resulting in DNA damage and genome instability. It has to be also considered that an oxidant/antioxidant imbalance may be connected to the complex mechanism leading to the DNA damage indicated in the blood of patients with head and neck cancer.

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“…g-radiation indicated higher level of endogenous DNA damage and decreased efficacy of DNA repair in comparison to lymphocytes taken from healthy donors. After 15 min of repair incubation, a higher level of DNA damage and slower DNA recovery than in lymphocytes taken from control subjects was observed (Saha et al, 2008). Palyvoda et al (2002) also showed decreased repair in HNSCC patients.…”

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confidence: 94%

Evaluation of DNA Double Strand Breaks Repair Efficiency in Head and Neck Cancer

Walczak

Rusin

Dziki

et al. 2012

DNA and Cell Biology

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Head and neck cancers (head and neck squamous cell carcinomas [HNSCC]) are a heterogeneous group of neoplasms with varying presenting symptoms, treatment, and expected outcome. There is a need to find an effective way of its treatment at the molecular level. Thus, we should identify the mechanism of cancer cell response to damaging agents' activity, especially at DNA level. Our major goal was to evaluate the efficacy of DNA double strand breaks (DSBs) repair in HTB-43 and SCC-25 cancer cell lines as well as lymphocytes taken from HNSCC patients and healthy donors. The DNA repair efficiency was measured by neutral comet assay as well as extrachromosomal assay for DNA DSBs repair (TAK assay). We determined the levels of two main pathways of DNA DSBs-nonhomologous end joining (NHEJ) and homologous recombination repair (HRR). Neutral comet assay was used for evaluation of DNA DSBs repair after treatment with genotoxic agents. DNA DSBs induced by gamma radiation were repaired slower in lymphocytes from HNSCC patients than in lymphocytes from healthy controls. HTB-43 and SCC-25 cancer cell lines have higher efficacy of NHEJ and HRR than lymphocytes taken from patients as well as control subjects. Our results confirm the necessity of further studies on the mechanisms of DNA DSBs repair to provide insight into the molecular basis of head and neck cancer, which will allow us to improve methods of HNSCC treatment.

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Quantification of DNA repair capacity in whole blood of patients with head and neck cancer and healthy donors by comet assay

Cited by 26 publications

References 29 publications

DNA repair deficiency in peripheral blood lymphocytes of endometrial cancer patients with a family history of cancer

DNA repair deficiency in peripheral blood lymphocytes of endometrial cancer patients with a family history of cancer

DNA Damage and Oxidant-Antioxidant Status in Blood of Patients with Head and Neck Cancer

Evaluation of DNA Double Strand Breaks Repair Efficiency in Head and Neck Cancer

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