Quantification of Diethyl Phthalate by a Rapid and Homogenous Fluorescence Polarization Immunoassay

Tian, Xi; Dong, Yaqing; Wang, Yufen; Song, Zhaorui; Meng, Meng; Eremin, Sergei A.; Chuan, Deng; Yin, Yongmei; Xi, Rimo

doi:10.1080/00032719.2015.1060600

Cited by 16 publications

(7 citation statements)

References 26 publications

(21 reference statements)

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“…The procedure was based on the azo coupling reaction, when the primary amino group of the hapten reacted with the carboxyl group of the protein as a carrier [ 30 ]. For this purpose, 12.5 µL of 31% hydrochloric acid, 375 µL of H 2 O, and 15 µL of Tween-20 were added at 0 °C to 5.8 mg of amino-dibutyl phthalate in 25 µL dimethylsulfoxide (DMSO), with the followed addition of 500 µL of water containing 1.375 mg of NaNO 2 .…”

Section: Methodsmentioning

confidence: 99%

Development of Lateral Flow Test-System for the Immunoassay of Dibutyl Phthalate in Natural Waters

et al. 2022

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The use of a large amount of toxic synthetic materials leads to an increase in the pollution of environmental objects. Phthalates are compounds structurally related to esters of phthalic acid that are widely used in the manufacturing of synthetic packaging materials as plasticizers. Their danger is conditioned by leaching into the environment and penetrating into living organisms with negative consequences and effects on various organs and tissues. This work presents the first development of lateral flow immunoassay to detect dibutyl phthalate, one of the most common representatives of the phthalates group. To form a test zone, a hapten–protein conjugate was synthesized, and gold nanoparticles conjugated with antibodies to dibutyl phthalate were used as a detecting conjugate. The work includes the preparation of immunoreagents, selectivity investigation, and the study of the characteristics of the medium providing a reliable optical signal. Under the selected conditions for the analysis, the detection limit was 33.4 ng/mL, and the working range of the determined concentrations was from 42.4 to 1500 ng/mL. Time of the assay–15 min. The developed technique was successfully applied to detect dibutyl phthalate in natural waters with recovery rates from 75 to 115%.

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Section: Methodsmentioning

confidence: 99%

Development of Lateral Flow Test-System for the Immunoassay of Dibutyl Phthalate in Natural Waters

et al. 2022

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“…To date, numerous analytical methods for detection of phthalate esters have been developed, and various detection methods have been developed in different samples such as plastic products, cosmetic products and drinking water (Tian et al, 2015;Vavrous et al, 2019;Yan et al, 2018). These existing analytical methods, including instrumental analysis, magnetic material sensor and fluorescent sensor, showed high sensitivity.…”

Section: Improving the Efficiency And Specificity Of The Existing Pae Detection Strategiesmentioning

confidence: 99%

Development of immunoassays for the determination of phthalates

Zhang

2020

Food and Agricultural Immunology

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The development of highly selective analytical methodologies to monitoring phthalates (phthalic acid esters, PAEs) is important for the safety of environment, food and equipment. As one of the most important and classical interactions in biological systems, antigen-antibody interactions constitute a highly specific chemical nature in biological systems, which inspire us to develop highly specific, highly selective and high-throughput immunoassays for monitoring PAEs in different fields. We here attempt to give an introduction of the recent development particularly focusing on the immunoassay based on the specific binding of antigen-antibody for detecting PAEs. We also present an overview of the potential applications of the antibody labelling strategies to immunoassays for PAE investigations. Finally, we present the major challenges and opportunities regarding the high selectivity and specificity of PAE analysis based on the antigen-antibody interaction. We believe that this review provides critical insights into highly selective PAE analysis and gives an understanding of immunoassays.

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“…The time that provided a stable FP signal was selected as optimum. [23] The competitive binding assay was conducted in a customized cuvette, with 8 nM of Dex-fl, 8 nM of diluted mPPARα-LBD*, and different concentrations of BPs. After an incubation at room temperature, the FP values were measured by fluorescence spectrophotometer (F-7000, Hitachi, Tokyo, Japan) at λ ex = 484, and λ em = 520.…”

Section: Competitive Fp Assaymentioning

confidence: 99%

Fluorescence polarization assay for the simultaneous determination of bisphenol A, bisphenol F and their diglycidyl ethers in canned tuna

Guan

Zhang

et al. 2017

International Journal of Food Properties

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This work developed a fluorescence polarization (FP) assay for simultaneous monitoring BPA, BPF, BADGE, and BFDGE in canned tuna. The assay was based on the competitive binding between bisphenol analogues (BPs) and dexamethasone fluorescein (Dex-fl) for mouse peroxisome proliferator-activated receptor α ligand binding domain (mPPARα-LBD*). The soluble form of mPPARα-LBD* was expressed in Escherichia coli strain Rosetta (DE3), as a recognition element on detection of BPs. Under optimized conditions, four bisphenol analytes were detected at concentrations corresponding to the specific migration limits (SMLs) as required by the European Union. The FP assay showed IC 50 values of 2.80, 6.51, 1.72, and 4.28 mg L −1 with a limit of detection of 0.35, 0.08, 0.10, and 0.49 mg L −1 for BPA, BPF, BADGE, and BFDGE, respectively. The analysis of spiked canned tuna samples showed the acceptable recoveries ranged from 87.9% to 77.3%, with variation coefficients ranging from 9.0% to 15.8%. With the high sensitivity and wide-range affinities to BPs, the developed assay based on mPPARα-LBD* exhibited the potential to be a screening assay for fast detection of BPs in canned tuna.

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Quantification of Diethyl Phthalate by a Rapid and Homogenous Fluorescence Polarization Immunoassay

Cited by 16 publications

References 26 publications

Development of Lateral Flow Test-System for the Immunoassay of Dibutyl Phthalate in Natural Waters

Development of Lateral Flow Test-System for the Immunoassay of Dibutyl Phthalate in Natural Waters

Development of immunoassays for the determination of phthalates

Fluorescence polarization assay for the simultaneous determination of bisphenol A, bisphenol F and their diglycidyl ethers in canned tuna

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