Quantification of dental erosion—A comparison of stylus profilometry and confocal laser scanning microscopy (CLSM)

Heurich, Erik; Beyer, Markus; Jandt, Klaus D.; Reichert, J.; Herold, Volker; Schnabelrauch, Matthias; Sigusch, Bernd W.

doi:10.1016/j.dental.2009.12.001

Cited by 71 publications

(80 citation statements)

References 28 publications

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“…On the other hand, a different situation was noticed when the solution was stirred for homogenization prior to the acquisition of a new image (d). In the beginning of this experiment the current is similar to the one obtained in the bulk solution, but its decay is very fast probably because of the removal of the chemical resistant enamel outer layer [6,23]. A final experiment (image not shown) was carried out using an acetate buffer solution of the same pH = 3.4.…”

Section: Secm Imagesmentioning

confidence: 99%

“…Then, the microelectrode surface was rinsed with water and sonicated for 5 minutes in distilled water. The radius of the microelectrode was determined by measuring the steady-state current in a 10 mmol L −1 K 3 Fe(CN) 6 solution containing 0.1 mol L −1 KCl as supporting electrolyte [17].…”

Section: Microelectrode Fabricationmentioning

confidence: 99%

“…These acid substances slowly soften the tooth surface, making it more susceptible to abrasion and contributing to the loss of the chemical structure. Erosion is the loss of tooth enamel, the hard, protective coating of the tooth, composed of impure calcium hydroxyapatite, Ca 10 (PO 4 ) 6 (OH) 2 [1]. The process is caused by acid attack that does not involve bacteria and results in tooth wear of dentin hypersensitivity, loss of form and color of the tooth, which may require restorative complex interventions [2].…”

Section: Introductionmentioning

confidence: 99%

“…Atomic force microscopy was used by Quartarone et al in an in vitro study, where the surface roughness of teeth samples kept in contact with acid drinks was evaluated and the results showed deep holes in the structure of enamel [5]. Heurich et al using confocal laser scanning microscopy, a technique widely used to quantitatively analyze enamel surfaces eroded by acidic solution, obtained quickly and accurately single 3D image topographic differences between an eroded enamel surface and a given reference area [6].…”

Section: Introductionmentioning

confidence: 99%

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Scanning Electrochemical Microscopy as a Tool for the Characterization of Dental Erosion

Castro

Lima

Ferreira

et al. 2011

International Journal of Electrochemistry

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When the tooth is exposed to acidic environments, an irreversible loss of dental hard tissue occurs in a process called dental erosion. In this work, the scanning electrochemical microscopy (SECM) was used to probe the consumption of protons at the vicinity of a tooth surface with a platinum microelectrode fixed at −0.5 (V) versus Ag/AgCl/KCl (sat) . SECM approach curves were recorded to assess the extent of diffusion in the solution close to the tooth substrate. SECM images clearly demonstrated that the acid erosion process is very fast at solution pH values in the range between 3 and 4.

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Section: Secm Imagesmentioning

confidence: 99%

Section: Microelectrode Fabricationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Scanning Electrochemical Microscopy as a Tool for the Characterization of Dental Erosion

Castro

Lima

Ferreira

et al. 2011

International Journal of Electrochemistry

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“…Citric acid has been applied in many erosion studies using different concentrations and different experimental set-ups with various analytical methods used for measuring the erosive effect [5,6].…”

Section: Introductionmentioning

confidence: 99%

Chelating effect of citric acid is negligible for development of enamel erosions

2015

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AIM Citric acid (CA) is a component in beverages responsible for dental erosion. The aim of this study was to examine the influence of CA with different pH, titratable acid and buffer capacity (ß), and the impact of the chelating effect of CA on development of enamel erosions. MATERIAL AND METHODS In a superfusion model, hydroxy apatite (HAp) dissolution of bovine enamel was measured in four experiments (EXP 1-4) with 27 experimental groups (n = 8 per group). The samples were superfused with different CA variations and respective controls. EXP-1: Dilution series of HCl (pH 2.15-3.02). EXP-2: Dilution series of natural CA (56-1.75 mmol l(-1); pH 2.15-3.02). EXP-3: CA solutions (56 and 14 mmol l(-1), ß: 39.7 and 10.2 mmol l(-1) pH(-1), respectively) with different titratable acidity at equal pH values. EXP-4: CA concentrations (56-1.75 mmol l(-1)) neutralized to pH 7. RESULTS CA led to higher HAp-dissolution than HCl. With higher pH, the difference in HAp-dissolution rate between the two acids became increasingly smaller. At equal pH, HAp-dissolution was higher for the CA with the higher amount of titratable acid. However, no clear correlation between erosion and titratable acid or ß could be found. Only minimal amounts of HAp were dissolved by neutralized CA compared to CA with natural pH. CONCLUSION Under the chosen conditions chelating effects of CA do not have a relevant influence of HAp-dissolution of enamel. Moreover, amount of HAp-dissolution by CA is not attributed to a single factor alone. The interplay between the different parameters of CA seems to be responsible for its erosive potential. CLINICAL RELEVANCE The erosive potential of solutions containing citric acid with unknown concentrations could not be predicted using a single parameter alone, and should at best determined in experimental set-ups. Results: CA led to higher HAp-dissolution than HCl. With higher pH, the difference in HAp-dissolution rate between the two acids became increasingly smaller. At equal pH, HAp-dissolution was higher for the CA with the higher amount of titratable acid.However, no clear correlation between erosion and titratable acid or ß could be found. Only minimal amounts of HAp were dissolved by neutralized CA compared to CA with natural pH. Conclusion:Under the chosen conditions chelating effects of CA do not have a relevant influence of HAp-dissolution of enamel. Moreover, amount of HApdissolution by CA is not attributed to a single factor alone. The interplay between the different parameters of CA seems to be responsible for its erosive potential.Clinical relevance: The erosive potential of solutions containing citric acid with unknown concentrations could not be predicted using a single parameter alone, and should at best determined in experimental set-ups.3

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Energy dispersive X‐ray spectrometry study of the protective effects of fluoride varnish and gel on enamel erosion

Filho¹,

Sanches

Martin

et al. 2010

Microscopy Res & Technique

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Quantification of dental erosion—A comparison of stylus profilometry and confocal laser scanning microscopy (CLSM)

Cited by 71 publications

References 28 publications

Scanning Electrochemical Microscopy as a Tool for the Characterization of Dental Erosion

Scanning Electrochemical Microscopy as a Tool for the Characterization of Dental Erosion

Chelating effect of citric acid is negligible for development of enamel erosions

Energy dispersive X‐ray spectrometry study of the protective effects of fluoride varnish and gel on enamel erosion

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