Quantification of Cyclin B1 and p34cdc2 in Bovine Cumulus-Oocyte Complexes and Expression Mapping of Genes Involved in the Cell Cycle by Complementary DNA Macroarrays1

Robert, Claude; Hue, Isabelle; McGraw, Serge; Gagné, Dominic; Sirard, Marc‐André

doi:10.1095/biolreprod.102.002147

Cited by 48 publications

(42 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cyclin B1-GFP distribution during meiotic maturation 159 abundance has been reported in other species including Xenopus (Pines & Hunt 1987, Li et al 1997) and bovine (Levesque & Sirard 1996, Robert et al 2002 but, in these species, it was consistent with the need for new cyclin B synthesis to induce GVBD. In contrast, mouse oocytes do not require new protein synthesis to undergo GVBD (Clarke & Masui 1983) and have been reported to have a sevenfold abundance of cyclin B1 over cdk1 (Kanatsu-Shinohara et al 2000).…”

Section: Exogenous Cyclin Stimulates Progression Through Meiosissupporting

confidence: 70%

The dynamics of cyclin B1 distribution during meiosis I in mouse oocytes

Marangos

Carroll

2004

Reproduction

View full text Add to dashboard Cite

Cdk1-cyclin B1 kinase activity drives oocytes through meiotic maturation. It is regulated by the phosphorylation status of cdk1 and by its spatial organisation. Here we used a cyclin B1-green fluorescent protein (GFP) fusion protein to examine the dynamics of cdk1-cyclin B1 distribution during meiosis I (MI) in living mouse oocytes. Microinjection of cyclin B1-GFP accelerated germinal vesicle breakdown (GVBD) and, as previously described, overrides cAMP-mediated meiotic arrest. GVBD was pre-empted by a translocation of cyclin B1-GFP from the cytoplasm to the germinal vesicle (GV). After nuclear accumulation, cyclin B1-GFP localised to the chromatin. The localisation of cyclin B1-GFP is governed by nuclear import and export. In GV intact oocytes, cyclin export was demonstrated by showing that cyclin B1-GFP injected into the GV is exported to the cytoplasm while a similar size dextran is retained. Import was revealed by the finding that cyclin B1-GFP accumulated in the GV when export was inhibited using leptomycin B. These studies show that GVBD in mouse oocytes is sensitive to cyclin B1 abundance and that the changes in distribution of cyclin B1 contribute to progression through MI.

show abstract

Section: Exogenous Cyclin Stimulates Progression Through Meiosissupporting

confidence: 70%

The dynamics of cyclin B1 distribution during meiosis I in mouse oocytes

Marangos

Carroll

2004

Reproduction

View full text Add to dashboard Cite

show abstract

“…6,7 Most mammalian species other than rodents also require the protein synthesis for GVBD, 13,16,17 and the cyclin B synthesis is thought to be a trigger of GVBD in bovine oocytes. 18,19 In contrast, although the critical protein required for GVBD has not yet been identified in porcine oocytes, we have reported previously that a small amount of cyclin B2 was present in porcine immature oocytes, and the cyclin B synthesis was not always necessary for the induction of GVBD by an injection of Figure 5. Effects of pigWee1B antisense RNA (ASpigWee1B) injection on the meiotic arrest of porcine oocytes.…”

Section: Discussionmentioning

confidence: 89%

“…13,16,17 It has been reported that bovine immature oocytes are lack of cyclin B, and that cyclin B synthesis is the trigger for GVBD. 18,19 Porcine immature oocytes, in which cyclin B1 is absent and the cyclin B2 level is very low, also require protein synthesis for GVBD, and their cyclins B1 and B2 syntheses start around GVBD. [20][21][22] Because protein synthesis is prerequisite for the meiotic resumption in mammalian species other than rodents, it remains possible that the inhibitory phosphorylation of Cdc2 is not required for the meiotic arrest of follicular oocytes.…”

Section: Introductionmentioning

confidence: 99%

Critical effect of pigWee1B on the regulation of meiotic resumption in porcine immature oocytes

Shimaoka

Nishimura²,

Kanô³

et al. 2009

Cell Cycle

View full text Add to dashboard Cite

Porcine immature oocytes require protein synthesis for meiotic resumption, thus the importance of Cdc2 inhibitory phosphorylation in their meiotic arrest remains controversial. We examined the involvement of Cdc2 phosphorylation in the meiotic arrest of porcine oocytes with a special focus on Wee1B, an oocyte-specific Wee1 family member recently reported in mouse oocytes. We cloned a Wee1B homologue of pig by RT-PCR followed by 5'-and 3'-RACE. Overexpression of pigWee1B in porcine immature oocytes by the injection of pigWee1B mRNA almost completely blocked the germinal vesicle breakdown (GVBD) under the low cAMP concentration, which could not block their spontaneous meiotic resumption by itself. The MPF activation and cyclin B synthesis were inhibited in these oocytes. Conversely, downregulation of pigWee1B expression by the injection of specific antisense mRNA induced GVBD in the oocytes, the spontaneous meiotic resumption of which was blocked by the high concentration of cAMP (dbcAMP). In these oocytes, the MPF activity was elevated and cyclin B was accumulated. Downregulation of pigMyt1, another Wee1 family member, could not induce the GVBD under the same condition. The inhibition of tyrosine phosphatase by vanadate blocked the GVBD even in the pigWee1B-downregulated oocytes. These results suggest that the inhibitory phosphorylation of CDC2, which is catalyzed by pigWee1B, but not pigMyt1, is involved in the meiotic arrest of porcine oocytes, and that the inactivation of Wee1B in combination with the phosphatase activation induces the conversion of pre-MPF to the active MPF and starts the cyclin B synthesis, follwed by a further increase of MPF and meiotic resumption.

show abstract

“…Several studies have performed different analyses to validate the accuracy and precision of results obtained through microarray cross-species hybridization (Chismar et al 2002, Ji et al 2004, Renn et al 2004, Bar-Or et al 2006. Also, different microarray platforms have been used for cross-species hybridization: custom oligo arrays (Chalmers et al 2005), Affymetrix arrays (Shah et al 2004, Grigoryev et al 2005, cDNA microarray (Adjaye et al 2004, Donaldson et al 2005, and cDNA macroarray (Robert et al 2002, Dalbies-Tran & Mermillod 2003. Biologically meaningful information has been obtained from all these studies.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of oocyte transcript profiles reveals a high degree of conservation among species

et al. 2008

View full text Add to dashboard Cite

Cross-species comparison of gene expression is a powerful approach for discovering genes that have been conserved throughout evolution. Conserved genes are presumably very important in the mechanisms related to the unique molecular functions in oocytes. The objective of this study was to identify genes expressed in the oocyte and conserved across three diverse vertebrate species. We report the global gene expression profiles of Bos taurus and Xenopus laevis oocytes on an NIA mouse development microarray that consists of 60-mer oligonucleotide probes representing more than 20 000 mouse transcripts derived from stem cell, oocyte, and early embryo cDNA libraries. Analysis based on intensity values revealed that 9853 and 10 046 genes are expressed in bovine and Xenopus oocytes respectively. Furthermore, previously published microarray data on preimplantation development in the mouse were used for a comparative analysis of global oocyte gene expression profiles. Interestingly, a substantial proportion of the genes expressed in mouse oocytes is conserved between the three species (74%, 7275 genes). Moreover, functional annotation of these conserved oocyteexpressed genes confirmed that certain functions are conserved among the three species. RNA metabolism and cell cycle were among the over-represented Gene Ontology terms in the biological process category. Finally, a pattern-matching analysis identified 208 conserved maternally expressed genes. Results from these cross-species hybridizations allowed numerous genes expressed in oocytes and conserved between Mus musculus, B. taurus, and X. laevis to be identified. This comparative analysis of oocyte transcript profiles revealed a high degree of conservation among species.

show abstract

Quantification of Cyclin B1 and p34cdc2 in Bovine Cumulus-Oocyte Complexes and Expression Mapping of Genes Involved in the Cell Cycle by Complementary DNA Macroarrays1

Cited by 48 publications

References 52 publications

The dynamics of cyclin B1 distribution during meiosis I in mouse oocytes

The dynamics of cyclin B1 distribution during meiosis I in mouse oocytes

Critical effect of pigWee1B on the regulation of meiotic resumption in porcine immature oocytes

Comparative analysis of oocyte transcript profiles reveals a high degree of conservation among species

Contact Info

Product

Resources

About